Use of genomic fingerprinting techniques for revealing DNA polymorphism in Ginkgo biloba L., A medicinal woody species

TY - JOUR

T1 - Use of genomic fingerprinting techniques for revealing DNA polymorphism in Ginkgo biloba L., A medicinal woody species

AU - Hârţa, Monica

AU - Pamfil, Doru

AU - Ardelean, Marin

AU - Pedersen, Carsten

AU - Pop, Rodica

AU - Cordea, Mirela

PY - 2010/3/1

Y1 - 2010/3/1

N2 - The main goal of our study was to use two DNA fingerprinting techniques (RAPD and AFLP) for revealing the possible intraspecific genetic variation between Ginkgo biloba individuals grown in different sites. Ten of the twenty RAPD (Random Amplification of Polymorphic DNA ) decamer primers yielded scorable amplification patterns and generated polymorphic bands among the genotypes studied. Of the 165 AFLP (Amplified Fragment Length Polymorphism) primer combinations used, Ecs-AGG+MCG have revealed the largest number of polymorphic bands (96) among samples of Ginkgo biloba originating from Romania and Denmark. The least number of polymorfic bands (74) was detected using primer combination Ecs-AGG+MGA. Individuals of Ginkgo, analyzed by the techniques mentioned above, clustered into two main groups and the values of genetic similarity coefficients between data shows some differences at DNA molecular level.

AB - The main goal of our study was to use two DNA fingerprinting techniques (RAPD and AFLP) for revealing the possible intraspecific genetic variation between Ginkgo biloba individuals grown in different sites. Ten of the twenty RAPD (Random Amplification of Polymorphic DNA ) decamer primers yielded scorable amplification patterns and generated polymorphic bands among the genotypes studied. Of the 165 AFLP (Amplified Fragment Length Polymorphism) primer combinations used, Ecs-AGG+MCG have revealed the largest number of polymorphic bands (96) among samples of Ginkgo biloba originating from Romania and Denmark. The least number of polymorfic bands (74) was detected using primer combination Ecs-AGG+MGA. Individuals of Ginkgo, analyzed by the techniques mentioned above, clustered into two main groups and the values of genetic similarity coefficients between data shows some differences at DNA molecular level.

KW - Aflp

KW - DNA polymorphism

KW - Fingerprinting techniques

KW - Ginkgo biloba

KW - Rapd

UR - http://www.scopus.com/inward/record.url?scp=78651263380&partnerID=8YFLogxK

M3 - Journal article

AN - SCOPUS:78651263380

SN - 1224-5984

VL - 15

SP - 56

EP - 61

JO - Romanian Biotechnological Letters

JF - Romanian Biotechnological Letters

IS - SUPPL.2

ER -