Updating rDNA restriction enzyme maps of Tetrahymena reveals four new intron-containing species

Henrik Nielsen, E M Simon, J Engberg

11 Citations (Scopus)

Abstract

The extrachromosomal rDNA molecules from a number of Tetrahymena strains were characterized by restriction enzyme mapping using three different restriction enzymes combined with gel blotting and hybridization analysis. Strains from four out of six recently described species were found to contain an intron in the 26s rRNA coding region. The evolutionary relationship among the species of the T. pyriformis complex was examined on the basis of the rDNA maps with emphasis on similarities between two of the new species and the widely studied T. thermophila and T. pigmentosa. Examination of a large number of T. pigmentosa strains showed this species to exhibit an unusual polymorphism with respect to its rDNA. It is suggested that recombinational cross-over events play a role in the formation of new rDNA alleles in this species.
Original languageEnglish
JournalThe Journal of protozoology
Volume32
Issue number3
Pages (from-to)480-5
Number of pages5
ISSN0022-3921
Publication statusPublished - 1985

Fingerprint

Dive into the research topics of 'Updating rDNA restriction enzyme maps of Tetrahymena reveals four new intron-containing species'. Together they form a unique fingerprint.

Cite this