Ultrastructure and mitochondrial numbers in pre- and postpubertal pig oocytes

Hanne Skovsgaard Pedersen; Henrik Callesen; Peter Løvendahl; Fenghua Chen; Jens Randel Nyengaard; Nanett Kvist Nikolaisen; Peter Holm; Poul Hyttel

doi:10.1071/RD14220

Ultrastructure and mitochondrial numbers in pre- and postpubertal pig oocytes

Hanne Skovsgaard Pedersen, Henrik Callesen, Peter Løvendahl, Fenghua Chen, Jens Randel Nyengaard, Nanett Kvist Nikolaisen, Peter Holm, Poul Hyttel

3 Citations (Scopus)

Abstract

Prepubertal pig oocytes are associated with lower developmental competence. The aim of this experiment was to conduct an exhaustive survey of oocyte ultrastructure and to use a design-unbiased stereological approach to quantify the numerical density and total number of mitochondria in oocytes with different diameters from pre- and postpubertal pigs. The ultrastructure of smaller prepubertal immature oocytes indicated active cells in close contact with cumulus cells. The postpubertal oocytes were more quiescent cell types. The small prepubertal oocytes had a lower total mitochondrial number, but no differences were observed in mitochondrial densities between groups. Mature postpubertal oocytes adhered to the following characteristics: presence of metaphase II, lack of contact between cumulus cells and oocyte, absence of rough endoplasmic reticulum and Golgi complexes, peripheral location of cortical granules and central localisation of mitochondria, vesicles and lipid droplets. Prepubertal oocytes displayed more variation. The ultrastructure of large pre- and postpubertal oocytes was compatible with higher developmental competence, whereas that of smaller prepubertal oocytes could explain their reduced capacity. The higher number of mitochondria in large pre- and postpubertal oocytes could have an influence on oocyte competence, by increasing the pool of mitochondria available for early embryonic development.

Original language	English
Journal	Reproduction, Fertility and Development
Volume	28
Issue number	5
Pages (from-to)	586-598
Number of pages	13
ISSN	1031-3613
DOIs	https://doi.org/10.1071/RD14220
Publication status	Published - 2016

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title = "Ultrastructure and mitochondrial numbers in pre- and postpubertal pig oocytes",

abstract = "Prepubertal pig oocytes are associated with lower developmental competence. The aim of this experiment was to conduct an exhaustive survey of oocyte ultrastructure and to use a design-unbiased stereological approach to quantify the numerical density and total number of mitochondria in oocytes with different diameters from pre- and postpubertal pigs. The ultrastructure of smaller prepubertal immature oocytes indicated active cells in close contact with cumulus cells. The postpubertal oocytes were more quiescent cell types. The small prepubertal oocytes had a lower total mitochondrial number, but no differences were observed in mitochondrial densities between groups. Mature postpubertal oocytes adhered to the following characteristics: presence of metaphase II, lack of contact between cumulus cells and oocyte, absence of rough endoplasmic reticulum and Golgi complexes, peripheral location of cortical granules and central localisation of mitochondria, vesicles and lipid droplets. Prepubertal oocytes displayed more variation. The ultrastructure of large pre- and postpubertal oocytes was compatible with higher developmental competence, whereas that of smaller prepubertal oocytes could explain their reduced capacity. The higher number of mitochondria in large pre- and postpubertal oocytes could have an influence on oocyte competence, by increasing the pool of mitochondria available for early embryonic development.",

author = "Pedersen, {Hanne Skovsgaard} and Henrik Callesen and Peter L{\o}vendahl and Fenghua Chen and Nyengaard, {Jens Randel} and Nikolaisen, {Nanett Kvist} and Peter Holm and Poul Hyttel",

year = "2016",

doi = "10.1071/RD14220",

language = "English",

volume = "28",

pages = "586--598",

journal = "Reproduction, Fertility and Development",

issn = "1031-3613",

publisher = "C S I R O Publishing",

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T1 - Ultrastructure and mitochondrial numbers in pre- and postpubertal pig oocytes

AU - Pedersen, Hanne Skovsgaard

AU - Callesen, Henrik

AU - Løvendahl, Peter

AU - Chen, Fenghua

AU - Nyengaard, Jens Randel

AU - Nikolaisen, Nanett Kvist

AU - Holm, Peter

AU - Hyttel, Poul

PY - 2016

Y1 - 2016

N2 - Prepubertal pig oocytes are associated with lower developmental competence. The aim of this experiment was to conduct an exhaustive survey of oocyte ultrastructure and to use a design-unbiased stereological approach to quantify the numerical density and total number of mitochondria in oocytes with different diameters from pre- and postpubertal pigs. The ultrastructure of smaller prepubertal immature oocytes indicated active cells in close contact with cumulus cells. The postpubertal oocytes were more quiescent cell types. The small prepubertal oocytes had a lower total mitochondrial number, but no differences were observed in mitochondrial densities between groups. Mature postpubertal oocytes adhered to the following characteristics: presence of metaphase II, lack of contact between cumulus cells and oocyte, absence of rough endoplasmic reticulum and Golgi complexes, peripheral location of cortical granules and central localisation of mitochondria, vesicles and lipid droplets. Prepubertal oocytes displayed more variation. The ultrastructure of large pre- and postpubertal oocytes was compatible with higher developmental competence, whereas that of smaller prepubertal oocytes could explain their reduced capacity. The higher number of mitochondria in large pre- and postpubertal oocytes could have an influence on oocyte competence, by increasing the pool of mitochondria available for early embryonic development.

AB - Prepubertal pig oocytes are associated with lower developmental competence. The aim of this experiment was to conduct an exhaustive survey of oocyte ultrastructure and to use a design-unbiased stereological approach to quantify the numerical density and total number of mitochondria in oocytes with different diameters from pre- and postpubertal pigs. The ultrastructure of smaller prepubertal immature oocytes indicated active cells in close contact with cumulus cells. The postpubertal oocytes were more quiescent cell types. The small prepubertal oocytes had a lower total mitochondrial number, but no differences were observed in mitochondrial densities between groups. Mature postpubertal oocytes adhered to the following characteristics: presence of metaphase II, lack of contact between cumulus cells and oocyte, absence of rough endoplasmic reticulum and Golgi complexes, peripheral location of cortical granules and central localisation of mitochondria, vesicles and lipid droplets. Prepubertal oocytes displayed more variation. The ultrastructure of large pre- and postpubertal oocytes was compatible with higher developmental competence, whereas that of smaller prepubertal oocytes could explain their reduced capacity. The higher number of mitochondria in large pre- and postpubertal oocytes could have an influence on oocyte competence, by increasing the pool of mitochondria available for early embryonic development.

U2 - 10.1071/RD14220

DO - 10.1071/RD14220

M3 - Journal article

C2 - 25482576

SN - 1031-3613

VL - 28

SP - 586

EP - 598

JO - Reproduction, Fertility and Development

JF - Reproduction, Fertility and Development

IS - 5

ER -

Ultrastructure and mitochondrial numbers in pre- and postpubertal pig oocytes

Abstract

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