UBL5 is essential for pre-mRNA splicing and sister chromatid cohesion in human cells

Yasuyoshi Oka; Hanne Varmark; Kristoffer Vitting-Seerup; Petra Beli; Johannes Eichler Waage; Anna Hakobyan; Martin Mistrik; Chuna Ram Choudhary; Mikkel Rohde; Simon Bekker-Jensen; Niels Mailand

doi:10.15252/embr.201438679

UBL5 is essential for pre-mRNA splicing and sister chromatid cohesion in human cells

Yasuyoshi Oka, Hanne Varmark, Kristoffer Vitting-Seerup, Petra Beli, Johannes Eichler Waage, Anna Hakobyan, Martin Mistrik, Chuna Ram Choudhary, Mikkel Rohde, Simon Bekker-Jensen, Niels Mailand

26 Citations (Scopus)

Abstract

UBL5 is an atypical ubiquitin-like protein, whose function in metazoans remains largely unexplored. We show that UBL5 is required for sister chromatid cohesion maintenance in human cells. UBL5 primarily associates with spliceosomal proteins, and UBL5 depletion decreases pre-mRNA splicing efficiency, leading to globally enhanced intron retention. Defective sister chromatid cohesion is a general consequence of dysfunctional pre-mRNA splicing, resulting from the selective downregulation of the cohesion protection factor Sororin. As the UBL5 yeast orthologue, Hub1, also promotes spliceosome functions, our results show that UBL5 plays an evolutionary conserved role in pre-mRNA splicing, the integrity of which is essential for the fidelity of chromosome segregation.

Original language	English
Journal	E M B O Reports
Volume	15
Issue number	9
Pages (from-to)	956-964
Number of pages	9
ISSN	1469-221X
DOIs	https://doi.org/10.15252/embr.201438679
Publication status	Published - Sept 2014

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title = "UBL5 is essential for pre-mRNA splicing and sister chromatid cohesion in human cells",

abstract = "UBL5 is an atypical ubiquitin-like protein, whose function in metazoans remains largely unexplored. We show that UBL5 is required for sister chromatid cohesion maintenance in human cells. UBL5 primarily associates with spliceosomal proteins, and UBL5 depletion decreases pre-mRNA splicing efficiency, leading to globally enhanced intron retention. Defective sister chromatid cohesion is a general consequence of dysfunctional pre-mRNA splicing, resulting from the selective downregulation of the cohesion protection factor Sororin. As the UBL5 yeast orthologue, Hub1, also promotes spliceosome functions, our results show that UBL5 plays an evolutionary conserved role in pre-mRNA splicing, the integrity of which is essential for the fidelity of chromosome segregation.",

author = "Yasuyoshi Oka and Hanne Varmark and Kristoffer Vitting-Seerup and Petra Beli and Waage, {Johannes Eichler} and Anna Hakobyan and Martin Mistrik and Choudhary, {Chuna Ram} and Mikkel Rohde and Simon Bekker-Jensen and Niels Mailand",

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doi = "10.15252/embr.201438679",

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T1 - UBL5 is essential for pre-mRNA splicing and sister chromatid cohesion in human cells

AU - Oka, Yasuyoshi

AU - Varmark, Hanne

AU - Vitting-Seerup, Kristoffer

AU - Beli, Petra

AU - Waage, Johannes Eichler

AU - Hakobyan, Anna

AU - Mistrik, Martin

AU - Choudhary, Chuna Ram

AU - Rohde, Mikkel

AU - Bekker-Jensen, Simon

AU - Mailand, Niels

PY - 2014/9

Y1 - 2014/9

N2 - UBL5 is an atypical ubiquitin-like protein, whose function in metazoans remains largely unexplored. We show that UBL5 is required for sister chromatid cohesion maintenance in human cells. UBL5 primarily associates with spliceosomal proteins, and UBL5 depletion decreases pre-mRNA splicing efficiency, leading to globally enhanced intron retention. Defective sister chromatid cohesion is a general consequence of dysfunctional pre-mRNA splicing, resulting from the selective downregulation of the cohesion protection factor Sororin. As the UBL5 yeast orthologue, Hub1, also promotes spliceosome functions, our results show that UBL5 plays an evolutionary conserved role in pre-mRNA splicing, the integrity of which is essential for the fidelity of chromosome segregation.

AB - UBL5 is an atypical ubiquitin-like protein, whose function in metazoans remains largely unexplored. We show that UBL5 is required for sister chromatid cohesion maintenance in human cells. UBL5 primarily associates with spliceosomal proteins, and UBL5 depletion decreases pre-mRNA splicing efficiency, leading to globally enhanced intron retention. Defective sister chromatid cohesion is a general consequence of dysfunctional pre-mRNA splicing, resulting from the selective downregulation of the cohesion protection factor Sororin. As the UBL5 yeast orthologue, Hub1, also promotes spliceosome functions, our results show that UBL5 plays an evolutionary conserved role in pre-mRNA splicing, the integrity of which is essential for the fidelity of chromosome segregation.

U2 - 10.15252/embr.201438679

DO - 10.15252/embr.201438679

M3 - Journal article

C2 - 25092792

SN - 1469-221X

VL - 15

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EP - 964

JO - E M B O Reports

JF - E M B O Reports

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ER -

UBL5 is essential for pre-mRNA splicing and sister chromatid cohesion in human cells

Abstract

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