Typing of Pseudomonas aeruginosa from hemorrhagic pneumonia in mink (Neovison vison)

Charlotte Mark Salomonsen; Goncalo Espregueira Themudo; Lars Jelsbak; Søren Molin; Niels Høiby; Anne Sofie Vedsted Hammer

doi:10.1016/j.vetmic.2012.12.003

Typing of Pseudomonas aeruginosa from hemorrhagic pneumonia in mink (Neovison vison)

Charlotte Mark Salomonsen, Goncalo Espregueira Themudo, Lars Jelsbak, Søren Molin, Niels Høiby, Anne Sofie Vedsted Hammer

13 Citations (Scopus)

Abstract

Hemorrhagic pneumonia in mink (. Neovison vison) is caused by Pseudomonas aeruginosa and is an acute and fatal disease in farmed mink. Earlier work has demonstrated that some outbreaks of hemorrhagic pneumonia are caused by pathogenic strains while most outbreaks are caused by local strains. The objective of this study was to determine the genetic and geographical relationship among outbreaks of hemorrhagic pneumonia by pulsed-field gel electrophoresis typing of P. aeruginosa isolates. Furthermore, chosen isolates were typed by a commercial genotyping method based on single nucleotide polymorphisms (SNPs) and compared to a larger dataset of human and environmental origin. The bacterial isolates were obtained from diagnostic samples from 2002 to 2009 and contained 164 isolates from 95 outbreaks on 90 farms. Our results show that most outbreaks of hemorrhagic pneumonia in mink are caused by distinct strains of P. aeruginosa. We also identified related P. aeruginosa strains which, together with two prevalent but unrelated clones, caused one third of the outbreaks of hemorrhagic pneumonia supporting the sparse literature on this subject. None of the SNP typed strains were identified in a large dataset of human and environmental origin.

Original language	English
Journal	Veterinary Microbiology
Volume	163
Issue number	1-2
Pages (from-to)	103-109
Number of pages	7
ISSN	0378-1135
DOIs	https://doi.org/10.1016/j.vetmic.2012.12.003
Publication status	Published - 2013

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title = "Typing of Pseudomonas aeruginosa from hemorrhagic pneumonia in mink (Neovison vison)",

abstract = "Hemorrhagic pneumonia in mink (. Neovison vison) is caused by Pseudomonas aeruginosa and is an acute and fatal disease in farmed mink. Earlier work has demonstrated that some outbreaks of hemorrhagic pneumonia are caused by pathogenic strains while most outbreaks are caused by local strains. The objective of this study was to determine the genetic and geographical relationship among outbreaks of hemorrhagic pneumonia by pulsed-field gel electrophoresis typing of P. aeruginosa isolates. Furthermore, chosen isolates were typed by a commercial genotyping method based on single nucleotide polymorphisms (SNPs) and compared to a larger dataset of human and environmental origin. The bacterial isolates were obtained from diagnostic samples from 2002 to 2009 and contained 164 isolates from 95 outbreaks on 90 farms. Our results show that most outbreaks of hemorrhagic pneumonia in mink are caused by distinct strains of P. aeruginosa. We also identified related P. aeruginosa strains which, together with two prevalent but unrelated clones, caused one third of the outbreaks of hemorrhagic pneumonia supporting the sparse literature on this subject. None of the SNP typed strains were identified in a large dataset of human and environmental origin.",

author = "Salomonsen, {Charlotte Mark} and Themudo, {Goncalo Espregueira} and Lars Jelsbak and S{\o}ren Molin and Niels H{\o}iby and Hammer, {Anne Sofie Vedsted}",

year = "2013",

doi = "10.1016/j.vetmic.2012.12.003",

language = "English",

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T1 - Typing of Pseudomonas aeruginosa from hemorrhagic pneumonia in mink (Neovison vison)

AU - Salomonsen, Charlotte Mark

AU - Themudo, Goncalo Espregueira

AU - Jelsbak, Lars

AU - Molin, Søren

AU - Høiby, Niels

AU - Hammer, Anne Sofie Vedsted

PY - 2013

Y1 - 2013

N2 - Hemorrhagic pneumonia in mink (. Neovison vison) is caused by Pseudomonas aeruginosa and is an acute and fatal disease in farmed mink. Earlier work has demonstrated that some outbreaks of hemorrhagic pneumonia are caused by pathogenic strains while most outbreaks are caused by local strains. The objective of this study was to determine the genetic and geographical relationship among outbreaks of hemorrhagic pneumonia by pulsed-field gel electrophoresis typing of P. aeruginosa isolates. Furthermore, chosen isolates were typed by a commercial genotyping method based on single nucleotide polymorphisms (SNPs) and compared to a larger dataset of human and environmental origin. The bacterial isolates were obtained from diagnostic samples from 2002 to 2009 and contained 164 isolates from 95 outbreaks on 90 farms. Our results show that most outbreaks of hemorrhagic pneumonia in mink are caused by distinct strains of P. aeruginosa. We also identified related P. aeruginosa strains which, together with two prevalent but unrelated clones, caused one third of the outbreaks of hemorrhagic pneumonia supporting the sparse literature on this subject. None of the SNP typed strains were identified in a large dataset of human and environmental origin.

AB - Hemorrhagic pneumonia in mink (. Neovison vison) is caused by Pseudomonas aeruginosa and is an acute and fatal disease in farmed mink. Earlier work has demonstrated that some outbreaks of hemorrhagic pneumonia are caused by pathogenic strains while most outbreaks are caused by local strains. The objective of this study was to determine the genetic and geographical relationship among outbreaks of hemorrhagic pneumonia by pulsed-field gel electrophoresis typing of P. aeruginosa isolates. Furthermore, chosen isolates were typed by a commercial genotyping method based on single nucleotide polymorphisms (SNPs) and compared to a larger dataset of human and environmental origin. The bacterial isolates were obtained from diagnostic samples from 2002 to 2009 and contained 164 isolates from 95 outbreaks on 90 farms. Our results show that most outbreaks of hemorrhagic pneumonia in mink are caused by distinct strains of P. aeruginosa. We also identified related P. aeruginosa strains which, together with two prevalent but unrelated clones, caused one third of the outbreaks of hemorrhagic pneumonia supporting the sparse literature on this subject. None of the SNP typed strains were identified in a large dataset of human and environmental origin.

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DO - 10.1016/j.vetmic.2012.12.003

M3 - Journal article

C2 - 23273415

SN - 0378-1135

VL - 163

SP - 103

EP - 109

JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 1-2

ER -

Typing of Pseudomonas aeruginosa from hemorrhagic pneumonia in mink (Neovison vison)

Abstract

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