Translational control and differential RNA decay are key elements regulating postsegregational expression of the killer protein encoded by the parB locus of plasmid R1.

K Gerdes; K Helin; O W Christensen; A Løbner-Olesen

Translational control and differential RNA decay are key elements regulating postsegregational expression of the killer protein encoded by the parB locus of plasmid R1.

K Gerdes, K Helin, O W Christensen, A Løbner-Olesen

48 Citations (Scopus)

Abstract

The parB locus of plasmid R1, which mediates plasmid stability via postsegregational killing of plasmid-free cells, encodes two genes, hok and sok. The hok gene product is a potent cell-killing protein. The hok gene is regulated at the translational level by the sok gene-encoded repressor, a small anti-sense RNA complementary to the hok mRNA. The hok mRNA is extraordinarily stable, while the sok RNA decays rapidly. The mechanism of postsegregational killing is explained by the following model; the sok RNA molecule rapidly disappears in cells that have lost a parB-carrying plasmid, leading to translation of the stable hok mRNA. Consequently, the Hok protein is synthesized and killing of the plasmid-free cell follows.

Original language	English
Journal	Journal of Molecular Biology
Volume	203
Issue number	1
Pages (from-to)	119-29
Number of pages	10
ISSN	0022-2836
Publication status	Published - 1988

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Translational control and differential RNA decay are key elements regulating postsegregational expression of the killer protein encoded by the parB locus of plasmid R1. / Gerdes, K; Helin, K; Christensen, O W et al.

In: Journal of Molecular Biology, Vol. 203, No. 1, 1988, p. 119-29.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Translational control and differential RNA decay are key elements regulating postsegregational expression of the killer protein encoded by the parB locus of plasmid R1.",

abstract = "The parB locus of plasmid R1, which mediates plasmid stability via postsegregational killing of plasmid-free cells, encodes two genes, hok and sok. The hok gene product is a potent cell-killing protein. The hok gene is regulated at the translational level by the sok gene-encoded repressor, a small anti-sense RNA complementary to the hok mRNA. The hok mRNA is extraordinarily stable, while the sok RNA decays rapidly. The mechanism of postsegregational killing is explained by the following model; the sok RNA molecule rapidly disappears in cells that have lost a parB-carrying plasmid, leading to translation of the stable hok mRNA. Consequently, the Hok protein is synthesized and killing of the plasmid-free cell follows.",

author = "K Gerdes and K Helin and Christensen, {O W} and A L{\o}bner-Olesen",

note = "Keywords: Base Sequence; Chromosome Mapping; Escherichia coli; Gene Expression Regulation; Genes, Bacterial; Molecular Sequence Data; Promoter Regions (Genetics); Protein Biosynthesis; R Factors; RNA, Bacterial; RNA, Messenger; Rifampin; Suppression, Genetic",

year = "1988",

language = "English",

volume = "203",

pages = "119--29",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Academic Press",

number = "1",

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TY - JOUR

T1 - Translational control and differential RNA decay are key elements regulating postsegregational expression of the killer protein encoded by the parB locus of plasmid R1.

AU - Gerdes, K

AU - Helin, K

AU - Christensen, O W

AU - Løbner-Olesen, A

N1 - Keywords: Base Sequence; Chromosome Mapping; Escherichia coli; Gene Expression Regulation; Genes, Bacterial; Molecular Sequence Data; Promoter Regions (Genetics); Protein Biosynthesis; R Factors; RNA, Bacterial; RNA, Messenger; Rifampin; Suppression, Genetic

PY - 1988

Y1 - 1988

N2 - The parB locus of plasmid R1, which mediates plasmid stability via postsegregational killing of plasmid-free cells, encodes two genes, hok and sok. The hok gene product is a potent cell-killing protein. The hok gene is regulated at the translational level by the sok gene-encoded repressor, a small anti-sense RNA complementary to the hok mRNA. The hok mRNA is extraordinarily stable, while the sok RNA decays rapidly. The mechanism of postsegregational killing is explained by the following model; the sok RNA molecule rapidly disappears in cells that have lost a parB-carrying plasmid, leading to translation of the stable hok mRNA. Consequently, the Hok protein is synthesized and killing of the plasmid-free cell follows.

AB - The parB locus of plasmid R1, which mediates plasmid stability via postsegregational killing of plasmid-free cells, encodes two genes, hok and sok. The hok gene product is a potent cell-killing protein. The hok gene is regulated at the translational level by the sok gene-encoded repressor, a small anti-sense RNA complementary to the hok mRNA. The hok mRNA is extraordinarily stable, while the sok RNA decays rapidly. The mechanism of postsegregational killing is explained by the following model; the sok RNA molecule rapidly disappears in cells that have lost a parB-carrying plasmid, leading to translation of the stable hok mRNA. Consequently, the Hok protein is synthesized and killing of the plasmid-free cell follows.

M3 - Journal article

C2 - 2460630

SN - 0022-2836

VL - 203

SP - 119

EP - 129

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 1

ER -

Translational control and differential RNA decay are key elements regulating postsegregational expression of the killer protein encoded by the parB locus of plasmid R1.

Abstract

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