Transfer of gut microbiota from lean and obese mice to antibiotic-treated mice

Merete Ellekilde; Ellika Marie Selfjord; Christian Schiøth Larsen; Maja Jakesevic; Ida Rune; Britt Tranberg Christensen; Finn Kvist Vogensen; Dennis Sandris Nielsen; Martin Iain Bahl; Tine Rask Licht; Axel Kornerup Hansen; Camilla Hartmann Friis Hansen

doi:10.1038/srep05922

Transfer of gut microbiota from lean and obese mice to antibiotic-treated mice

Merete Ellekilde, Ellika Marie Selfjord, Christian Schiøth Larsen, Maja Jakesevic, Ida Rune, Britt Tranberg Christensen, Finn Kvist Vogensen, Dennis Sandris Nielsen, Martin Iain Bahl, Tine Rask Licht, Axel Kornerup Hansen, Camilla Hartmann Friis Hansen

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Abstract

Transferring gut microbiota from one individual to another may enable researchers to humanize the gut of animal models and transfer phenotypes between species. To date, most studies of gut microbiota transfer are performed in germ-free mice. In the studies presented, it was tested whether an antibiotic treatment approach could be used instead. C57BL/6 mice were treated with ampicillin prior to inoculation at weaning or eight weeks of age with gut microbiota from lean or obese donors. The gut microbiota and clinical parameters of the recipients was characterized one and six weeks after inoculation. The results demonstrate, that the donor gut microbiota was introduced, established, and changed the gut microbiota of the recipients. Six weeks after inoculation, the differences persisted, however alteration of the gut microbiota occurred with time within the groups. The clinical parameters of the donor phenotype were partly transmissible from obese to lean mice, in particularly β 2 cell hyperactivity in the obese recipients. Thus, a successful inoculation of gut microbiota was not age dependent in order for the microbes to colonize, and transferring different microbial compositions to conventional antibiotic-treated mice was possible at least for a time period during which the microbiota may permanently modulate important host functions.

Original language	English
Article number	5922
Journal	Scientific Reports
Volume	4
Number of pages	8
ISSN	2045-2322
DOIs	https://doi.org/10.1038/srep05922
Publication status	Published - 1 Aug 2014

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Ellekilde_2014_Transfer_of_gutFinal published version, 3.9 MBLicence: CC BY-NC-ND

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title = "Transfer of gut microbiota from lean and obese mice to antibiotic-treated mice",

abstract = "Transferring gut microbiota from one individual to another may enable researchers to humanize the gut of animal models and transfer phenotypes between species. To date, most studies of gut microbiota transfer are performed in germ-free mice. In the studies presented, it was tested whether an antibiotic treatment approach could be used instead. C57BL/6 mice were treated with ampicillin prior to inoculation at weaning or eight weeks of age with gut microbiota from lean or obese donors. The gut microbiota and clinical parameters of the recipients was characterized one and six weeks after inoculation. The results demonstrate, that the donor gut microbiota was introduced, established, and changed the gut microbiota of the recipients. Six weeks after inoculation, the differences persisted, however alteration of the gut microbiota occurred with time within the groups. The clinical parameters of the donor phenotype were partly transmissible from obese to lean mice, in particularly β 2 cell hyperactivity in the obese recipients. Thus, a successful inoculation of gut microbiota was not age dependent in order for the microbes to colonize, and transferring different microbial compositions to conventional antibiotic-treated mice was possible at least for a time period during which the microbiota may permanently modulate important host functions.",

author = "Merete Ellekilde and Selfjord, {Ellika Marie} and Larsen, {Christian Schi{\o}th} and Maja Jakesevic and Ida Rune and Christensen, {Britt Tranberg} and Vogensen, {Finn Kvist} and Nielsen, {Dennis Sandris} and Bahl, {Martin Iain} and Licht, {Tine Rask} and Hansen, {Axel Kornerup} and Hansen, {Camilla Hartmann Friis}",

year = "2014",

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doi = "10.1038/srep05922",

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T1 - Transfer of gut microbiota from lean and obese mice to antibiotic-treated mice

AU - Ellekilde, Merete

AU - Selfjord, Ellika Marie

AU - Larsen, Christian Schiøth

AU - Jakesevic, Maja

AU - Rune, Ida

AU - Christensen, Britt Tranberg

AU - Vogensen, Finn Kvist

AU - Nielsen, Dennis Sandris

AU - Bahl, Martin Iain

AU - Licht, Tine Rask

AU - Hansen, Axel Kornerup

AU - Hansen, Camilla Hartmann Friis

PY - 2014/8/1

Y1 - 2014/8/1

N2 - Transferring gut microbiota from one individual to another may enable researchers to humanize the gut of animal models and transfer phenotypes between species. To date, most studies of gut microbiota transfer are performed in germ-free mice. In the studies presented, it was tested whether an antibiotic treatment approach could be used instead. C57BL/6 mice were treated with ampicillin prior to inoculation at weaning or eight weeks of age with gut microbiota from lean or obese donors. The gut microbiota and clinical parameters of the recipients was characterized one and six weeks after inoculation. The results demonstrate, that the donor gut microbiota was introduced, established, and changed the gut microbiota of the recipients. Six weeks after inoculation, the differences persisted, however alteration of the gut microbiota occurred with time within the groups. The clinical parameters of the donor phenotype were partly transmissible from obese to lean mice, in particularly β 2 cell hyperactivity in the obese recipients. Thus, a successful inoculation of gut microbiota was not age dependent in order for the microbes to colonize, and transferring different microbial compositions to conventional antibiotic-treated mice was possible at least for a time period during which the microbiota may permanently modulate important host functions.

AB - Transferring gut microbiota from one individual to another may enable researchers to humanize the gut of animal models and transfer phenotypes between species. To date, most studies of gut microbiota transfer are performed in germ-free mice. In the studies presented, it was tested whether an antibiotic treatment approach could be used instead. C57BL/6 mice were treated with ampicillin prior to inoculation at weaning or eight weeks of age with gut microbiota from lean or obese donors. The gut microbiota and clinical parameters of the recipients was characterized one and six weeks after inoculation. The results demonstrate, that the donor gut microbiota was introduced, established, and changed the gut microbiota of the recipients. Six weeks after inoculation, the differences persisted, however alteration of the gut microbiota occurred with time within the groups. The clinical parameters of the donor phenotype were partly transmissible from obese to lean mice, in particularly β 2 cell hyperactivity in the obese recipients. Thus, a successful inoculation of gut microbiota was not age dependent in order for the microbes to colonize, and transferring different microbial compositions to conventional antibiotic-treated mice was possible at least for a time period during which the microbiota may permanently modulate important host functions.

U2 - 10.1038/srep05922

DO - 10.1038/srep05922

M3 - Journal article

C2 - 25082483

SN - 2045-2322

VL - 4

JO - Scientific Reports

JF - Scientific Reports

M1 - 5922

ER -

Transfer of gut microbiota from lean and obese mice to antibiotic-treated mice

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