The Vitamin D Analogue Calcipotriol Reduces the Frequency of CD8+IL-17+ T Cells in Psoriasis Lesions

B Dyring-Andersen; C M Bonefeld; M Bzorek; M B Løvendorf; J P H Lauritsen; Lone Skov; C Geisler

doi:10.1111/sji.12304

The Vitamin D Analogue Calcipotriol Reduces the Frequency of CD8⁺IL-17⁺ T Cells in Psoriasis Lesions

B Dyring-Andersen, C M Bonefeld, M Bzorek, M B Løvendorf, J P H Lauritsen, Lone Skov, C Geisler

22 Citations (Scopus)

Abstract

The vitamin D analogue calcipotriol is an immunomodulatory drug widely used to treat psoriasis; however, how calcipotriol affects the immune cells in psoriasis lesions is not fully understood. The aim of this atudy was to investigate the effect of calcipotriol on the frequency of CD4⁺ and CD8⁺ T cells and innate lymphoid cells (ILC) and their production of IL-17A, IFN-γ and IL-22 in psoriasis lesions in patients with chronic plaque psoriasis. Eighteen patients with psoriasis were included, and two similar psoriasis lesions were chosen for each patient. One lesion was treated with calcipotriol (50 μg/g) and the other with vehicle twice a day for 14 days. The clinical effect was measured by degree of erythema, scaling and induration in each lesion (SUM score). Skin biopsies were collected for histological and immunohistochemical analyses. Skin-derived cells were isolated and analysed by flow cytometry. After 14 days of treatment with calcipotriol, a significant clinical and histological effect was seen; however, we found no differences in the frequency of CD4⁺ and CD8⁺ T cells or ILC between calcipotriol- and vehicle-treated skin. The main finding was a significant decrease in CD8⁺IL-17⁺ T cells in skin-derived cells from calcipotriol-treated skin, which was further supported by the absence of CD8⁺IL-17⁺ T cells in immunohistochemical staining of calcipotriol-treated skin. No changes in the frequency of IL-22⁺ or IFN-γ⁺ cells were observed. Our findings show that the vitamin D analogue calcipotriol reduces the frequency of CD8⁺IL-17⁺ T cells in psoriasis lesions concomitant with clinical improvement.

Original language	English
Journal	Scandinavian Journal of Immunology
Volume	82
Issue number	1
Pages (from-to)	84-91
Number of pages	8
ISSN	0300-9475
DOIs	https://doi.org/10.1111/sji.12304
Publication status	Published - 1 Jul 2015

Keywords

Adult
Aged
CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes
Calcitriol
Dermatologic Agents
Erythema
Female
Humans
Interferon-gamma
Interleukin-17
Interleukins
Lymphocyte Count
Male
Middle Aged
Psoriasis
Young Adult

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10.1111/sji.12304

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Cite this

@article{037cc87deb374e43bc652e716c0f55ae,

title = "The Vitamin D Analogue Calcipotriol Reduces the Frequency of CD8+IL-17+ T Cells in Psoriasis Lesions",

abstract = "The vitamin D analogue calcipotriol is an immunomodulatory drug widely used to treat psoriasis; however, how calcipotriol affects the immune cells in psoriasis lesions is not fully understood. The aim of this atudy was to investigate the effect of calcipotriol on the frequency of CD4+ and CD8+ T cells and innate lymphoid cells (ILC) and their production of IL-17A, IFN-γ and IL-22 in psoriasis lesions in patients with chronic plaque psoriasis. Eighteen patients with psoriasis were included, and two similar psoriasis lesions were chosen for each patient. One lesion was treated with calcipotriol (50 μg/g) and the other with vehicle twice a day for 14 days. The clinical effect was measured by degree of erythema, scaling and induration in each lesion (SUM score). Skin biopsies were collected for histological and immunohistochemical analyses. Skin-derived cells were isolated and analysed by flow cytometry. After 14 days of treatment with calcipotriol, a significant clinical and histological effect was seen; however, we found no differences in the frequency of CD4+ and CD8+ T cells or ILC between calcipotriol- and vehicle-treated skin. The main finding was a significant decrease in CD8+IL-17+ T cells in skin-derived cells from calcipotriol-treated skin, which was further supported by the absence of CD8+IL-17+ T cells in immunohistochemical staining of calcipotriol-treated skin. No changes in the frequency of IL-22+ or IFN-γ+ cells were observed. Our findings show that the vitamin D analogue calcipotriol reduces the frequency of CD8+IL-17+ T cells in psoriasis lesions concomitant with clinical improvement.",

keywords = "Adult, Aged, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Calcitriol, Dermatologic Agents, Erythema, Female, Humans, Interferon-gamma, Interleukin-17, Interleukins, Lymphocyte Count, Male, Middle Aged, Psoriasis, Young Adult",

author = "B Dyring-Andersen and Bonefeld, {C M} and M Bzorek and L{\o}vendorf, {M B} and Lauritsen, {J P H} and Lone Skov and C Geisler",

note = "{\textcopyright} 2015 John Wiley & Sons Ltd.",

year = "2015",

month = jul,

day = "1",

doi = "10.1111/sji.12304",

language = "English",

volume = "82",

pages = "84--91",

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TY - JOUR

T1 - The Vitamin D Analogue Calcipotriol Reduces the Frequency of CD8+IL-17+ T Cells in Psoriasis Lesions

AU - Dyring-Andersen, B

AU - Bonefeld, C M

AU - Bzorek, M

AU - Løvendorf, M B

AU - Lauritsen, J P H

AU - Skov, Lone

AU - Geisler, C

PY - 2015/7/1

Y1 - 2015/7/1

N2 - The vitamin D analogue calcipotriol is an immunomodulatory drug widely used to treat psoriasis; however, how calcipotriol affects the immune cells in psoriasis lesions is not fully understood. The aim of this atudy was to investigate the effect of calcipotriol on the frequency of CD4+ and CD8+ T cells and innate lymphoid cells (ILC) and their production of IL-17A, IFN-γ and IL-22 in psoriasis lesions in patients with chronic plaque psoriasis. Eighteen patients with psoriasis were included, and two similar psoriasis lesions were chosen for each patient. One lesion was treated with calcipotriol (50 μg/g) and the other with vehicle twice a day for 14 days. The clinical effect was measured by degree of erythema, scaling and induration in each lesion (SUM score). Skin biopsies were collected for histological and immunohistochemical analyses. Skin-derived cells were isolated and analysed by flow cytometry. After 14 days of treatment with calcipotriol, a significant clinical and histological effect was seen; however, we found no differences in the frequency of CD4+ and CD8+ T cells or ILC between calcipotriol- and vehicle-treated skin. The main finding was a significant decrease in CD8+IL-17+ T cells in skin-derived cells from calcipotriol-treated skin, which was further supported by the absence of CD8+IL-17+ T cells in immunohistochemical staining of calcipotriol-treated skin. No changes in the frequency of IL-22+ or IFN-γ+ cells were observed. Our findings show that the vitamin D analogue calcipotriol reduces the frequency of CD8+IL-17+ T cells in psoriasis lesions concomitant with clinical improvement.

AB - The vitamin D analogue calcipotriol is an immunomodulatory drug widely used to treat psoriasis; however, how calcipotriol affects the immune cells in psoriasis lesions is not fully understood. The aim of this atudy was to investigate the effect of calcipotriol on the frequency of CD4+ and CD8+ T cells and innate lymphoid cells (ILC) and their production of IL-17A, IFN-γ and IL-22 in psoriasis lesions in patients with chronic plaque psoriasis. Eighteen patients with psoriasis were included, and two similar psoriasis lesions were chosen for each patient. One lesion was treated with calcipotriol (50 μg/g) and the other with vehicle twice a day for 14 days. The clinical effect was measured by degree of erythema, scaling and induration in each lesion (SUM score). Skin biopsies were collected for histological and immunohistochemical analyses. Skin-derived cells were isolated and analysed by flow cytometry. After 14 days of treatment with calcipotriol, a significant clinical and histological effect was seen; however, we found no differences in the frequency of CD4+ and CD8+ T cells or ILC between calcipotriol- and vehicle-treated skin. The main finding was a significant decrease in CD8+IL-17+ T cells in skin-derived cells from calcipotriol-treated skin, which was further supported by the absence of CD8+IL-17+ T cells in immunohistochemical staining of calcipotriol-treated skin. No changes in the frequency of IL-22+ or IFN-γ+ cells were observed. Our findings show that the vitamin D analogue calcipotriol reduces the frequency of CD8+IL-17+ T cells in psoriasis lesions concomitant with clinical improvement.

KW - Adult

KW - Aged

KW - CD4-Positive T-Lymphocytes

KW - CD8-Positive T-Lymphocytes

KW - Calcitriol

KW - Dermatologic Agents

KW - Erythema

KW - Female

KW - Humans

KW - Interferon-gamma

KW - Interleukin-17

KW - Interleukins

KW - Lymphocyte Count

KW - Male

KW - Middle Aged

KW - Psoriasis

KW - Young Adult

U2 - 10.1111/sji.12304

DO - 10.1111/sji.12304

M3 - Journal article

C2 - 25904071

SN - 0300-9475

VL - 82

SP - 84

EP - 91

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

IS - 1