The use of sequential organ failure assessment parameters in an awake porcine model of severe Staphylococcus aureus sepsis

Karen Elisabeth Sørensen; Ole Lerberg Nielsen; Malene Muusfeldt Birck; Dorte Bratbo Sørensen; Páll S. Leifsson; Henrik Elvang Jensen; Bent Aalbæk; Annemarie Thuri Kristensen; Bo Wiinberg; Mads Kjelgaard-Hansen; Peter M. H. Heegaard; Tine Moesgaard Iburg

doi:10.1111/j.1600-0463.2012.02917.x

The use of sequential organ failure assessment parameters in an awake porcine model of severe Staphylococcus aureus sepsis

Karen Elisabeth Sørensen, Ole Lerberg Nielsen, Malene Muusfeldt Birck, Dorte Bratbo Sørensen, Páll S. Leifsson, Henrik Elvang Jensen, Bent Aalbæk, Annemarie Thuri Kristensen, Bo Wiinberg, Mads Kjelgaard-Hansen, Peter M. H. Heegaard, Tine Moesgaard Iburg

16 Citations (Scopus)

Abstract

The human sequential organ failure assessment (SOFA) scoring system is used worldwide in intensive care units for assessing the extent of organ dysfunction/failure in patients with severe sepsis. An increasing number of septic cases are caused by Gram-positive bacteria as Staphylococcus aureus. The aim of the current study was to apply the human SOFA parameters in an awake, porcine model of severe S. aureus sepsis. Five pigs were inoculated intravenously with S. aureus and two control animals were sham-inoculated. Extensive clinical monitoring and sequential blood sampling was obtained and analysed for SOFA parameters. Dysfunction/failure was observed in the respiratory, haemostatic and hepatic system of all infected animals, together with initial cardiovascular dysfunction. The pulmonary system was the first to fail clinically, which corresponds with similar human findings, whereas the liver was affected earlier in pigs compared to humans. The use of human SOFA parameters was valuable in identifying dysfunctional/failing organs and showed consistency between this porcine model and human severe sepsis. Applying SOFA parameters in this model increased the relevance for comparison to clinical methods of evaluating human severe sepsis. Changes in SOFA parameters may in future porcine studies serve as a target for monitoring the effect of therapeutic intervention.

Original language	English
Journal	A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
Volume	120
Issue number	11
Pages (from-to)	909-921
Number of pages	13
ISSN	0903-4641
DOIs	https://doi.org/10.1111/j.1600-0463.2012.02917.x
Publication status	Published - Nov 2012

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Sørensen, K. E., Nielsen, O. L., Birck, M. M., Sørensen, D. B., Leifsson, P. S., Jensen, H. E., Aalbæk, B., Kristensen, A. T., Wiinberg, B., Kjelgaard-Hansen, M., Heegaard, P. M. H., & Iburg, T. M. (2012). The use of sequential organ failure assessment parameters in an awake porcine model of severe Staphylococcus aureus sepsis. A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, 120(11), 909-921. https://doi.org/10.1111/j.1600-0463.2012.02917.x

The use of sequential organ failure assessment parameters in an awake porcine model of severe Staphylococcus aureus sepsis. / Sørensen, Karen Elisabeth; Nielsen, Ole Lerberg; Birck, Malene Muusfeldt et al.
In: A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, Vol. 120, No. 11, 11.2012, p. 909-921.

Research output: Contribution to journal › Journal article › Research › peer-review

Sørensen, KE, Nielsen, OL, Birck, MM, Sørensen, DB , Leifsson, PS , Jensen, HE , Aalbæk, B , Kristensen, AT , Wiinberg, B , Kjelgaard-Hansen, M, Heegaard, PMH & Iburg, TM 2012, 'The use of sequential organ failure assessment parameters in an awake porcine model of severe Staphylococcus aureus sepsis', A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, vol. 120, no. 11, pp. 909-921. https://doi.org/10.1111/j.1600-0463.2012.02917.x

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title = "The use of sequential organ failure assessment parameters in an awake porcine model of severe Staphylococcus aureus sepsis",

abstract = "The human sequential organ failure assessment (SOFA) scoring system is used worldwide in intensive care units for assessing the extent of organ dysfunction/failure in patients with severe sepsis. An increasing number of septic cases are caused by Gram-positive bacteria as Staphylococcus aureus. The aim of the current study was to apply the human SOFA parameters in an awake, porcine model of severe S. aureus sepsis. Five pigs were inoculated intravenously with S. aureus and two control animals were sham-inoculated. Extensive clinical monitoring and sequential blood sampling was obtained and analysed for SOFA parameters. Dysfunction/failure was observed in the respiratory, haemostatic and hepatic system of all infected animals, together with initial cardiovascular dysfunction. The pulmonary system was the first to fail clinically, which corresponds with similar human findings, whereas the liver was affected earlier in pigs compared to humans. The use of human SOFA parameters was valuable in identifying dysfunctional/failing organs and showed consistency between this porcine model and human severe sepsis. Applying SOFA parameters in this model increased the relevance for comparison to clinical methods of evaluating human severe sepsis. Changes in SOFA parameters may in future porcine studies serve as a target for monitoring the effect of therapeutic intervention.",

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AU - Jensen, Henrik Elvang

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AU - Kristensen, Annemarie Thuri

AU - Wiinberg, Bo

AU - Kjelgaard-Hansen, Mads

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AB - The human sequential organ failure assessment (SOFA) scoring system is used worldwide in intensive care units for assessing the extent of organ dysfunction/failure in patients with severe sepsis. An increasing number of septic cases are caused by Gram-positive bacteria as Staphylococcus aureus. The aim of the current study was to apply the human SOFA parameters in an awake, porcine model of severe S. aureus sepsis. Five pigs were inoculated intravenously with S. aureus and two control animals were sham-inoculated. Extensive clinical monitoring and sequential blood sampling was obtained and analysed for SOFA parameters. Dysfunction/failure was observed in the respiratory, haemostatic and hepatic system of all infected animals, together with initial cardiovascular dysfunction. The pulmonary system was the first to fail clinically, which corresponds with similar human findings, whereas the liver was affected earlier in pigs compared to humans. The use of human SOFA parameters was valuable in identifying dysfunctional/failing organs and showed consistency between this porcine model and human severe sepsis. Applying SOFA parameters in this model increased the relevance for comparison to clinical methods of evaluating human severe sepsis. Changes in SOFA parameters may in future porcine studies serve as a target for monitoring the effect of therapeutic intervention.

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JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

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The use of sequential organ failure assessment parameters in an awake porcine model of severe Staphylococcus aureus sepsis

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