The transcriptional landscape

Henrik Nielsen

doi:10.1007/978-1-59745-248-9_1

The transcriptional landscape

Henrik Nielsen

Department of Cellular and Molecular Medicine

3 Citations (Scopus)

Abstract

The application of new and less biased methods to study the transcriptional output from genomes, such as tiling arrays and deep sequencing, has revealed that most of the genome is transcribed and that there is substantial overlap of transcripts derived from the two strands of DNA. In protein coding regions, the map of transcripts is very complex due to small transcripts from the flanking ends of the transcription unit, the use of multiple start and stop sites for the main transcript, production of multiple functional RNA molecules from the same primary transcript, and RNA molecules made by independent transcription from within the unit. In genomic regions separating those that encode proteins or highly abundant RNA molecules with known function, transcripts are generally of low abundance and short-lived. In most of these cases, it is unclear to what extent a function is related to transcription per se or to the RNA products.

Original language	English
Title of host publication	Methods in Molecular Biology : RNA: Methods and Protocols
Number of pages	12
Volume	703
Publisher	Springer Science+Business Media
Publication date	1 Jan 2011
Pages	3-14
Chapter	1
DOIs	https://doi.org/10.1007/978-1-59745-248-9_1
Publication status	Published - 1 Jan 2011

Keywords

Gene Expression Profiling
Genome
Genome Components
Promoter Regions, Genetic

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10.1007/978-1-59745-248-9_1

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AB - The application of new and less biased methods to study the transcriptional output from genomes, such as tiling arrays and deep sequencing, has revealed that most of the genome is transcribed and that there is substantial overlap of transcripts derived from the two strands of DNA. In protein coding regions, the map of transcripts is very complex due to small transcripts from the flanking ends of the transcription unit, the use of multiple start and stop sites for the main transcript, production of multiple functional RNA molecules from the same primary transcript, and RNA molecules made by independent transcription from within the unit. In genomic regions separating those that encode proteins or highly abundant RNA molecules with known function, transcripts are generally of low abundance and short-lived. In most of these cases, it is unclear to what extent a function is related to transcription per se or to the RNA products.

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KW - Genome

KW - Genome Components

KW - Promoter Regions, Genetic

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ER -

The transcriptional landscape

Abstract

Keywords

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