The tetraspanin CD9 affords high-purity capture of all murine hematopoietic stem cells

Göran Karlsson; Emma Rörby; Cristina Pina; Shamit Soneji; Kristian Reckzeh; Kenichi Miharada; Christine Karlsson; Yanping Guo; Cristina Fugazza; Rajeev Gupta; Joost H A Martens; Hendrik G Stunnenberg; Stefan Karlsson; Tariq Enver

doi:10.1016/j.celrep.2013.07.020

The tetraspanin CD9 affords high-purity capture of all murine hematopoietic stem cells

Göran Karlsson, Emma Rörby, Cristina Pina, Shamit Soneji, Kristian Reckzeh, Kenichi Miharada, Christine Karlsson, Yanping Guo, Cristina Fugazza, Rajeev Gupta, Joost H A Martens, Hendrik G Stunnenberg, Stefan Karlsson, Tariq Enver

27 Citations (Scopus)

Abstract

Prospective isolation is critical for understanding the cellular and molecular aspects of stem cell heterogeneity. Here, we identify the cell surface antigen CD9 as a positive marker that provides a simple alternative for hematopoietic stem cell isolation at high purity. Crucially, CD9 affords the capture of all hematopoietic stem cells in murine bone marrow in the absence of contaminating populations that lack authentic stem cell function. Using CD9 as a tool to subdivide hematopoietic stem-cell-containing populations, we provide evidence for heterogeneity at the cellular, functional, and molecular levels.

Original language	English
Journal	Cell Reports
Volume	4
Issue number	4
Pages (from-to)	642-8
Number of pages	7
ISSN	2211-1247
DOIs	https://doi.org/10.1016/j.celrep.2013.07.020
Publication status	Published - 29 Aug 2013
Externally published	Yes

Keywords

Animals
Biomarkers/analysis
Cell Separation/methods
Flow Cytometry/methods
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells/chemistry
Mice
Mice, Inbred C57BL
Tetraspanin-29/analysis

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title = "The tetraspanin CD9 affords high-purity capture of all murine hematopoietic stem cells",

abstract = "Prospective isolation is critical for understanding the cellular and molecular aspects of stem cell heterogeneity. Here, we identify the cell surface antigen CD9 as a positive marker that provides a simple alternative for hematopoietic stem cell isolation at high purity. Crucially, CD9 affords the capture of all hematopoietic stem cells in murine bone marrow in the absence of contaminating populations that lack authentic stem cell function. Using CD9 as a tool to subdivide hematopoietic stem-cell-containing populations, we provide evidence for heterogeneity at the cellular, functional, and molecular levels. ",

keywords = "Animals, Biomarkers/analysis, Cell Separation/methods, Flow Cytometry/methods, Hematopoietic Stem Cell Transplantation, Hematopoietic Stem Cells/chemistry, Mice, Mice, Inbred C57BL, Tetraspanin-29/analysis",

author = "G{\"o}ran Karlsson and Emma R{\"o}rby and Cristina Pina and Shamit Soneji and Kristian Reckzeh and Kenichi Miharada and Christine Karlsson and Yanping Guo and Cristina Fugazza and Rajeev Gupta and Martens, {Joost H A} and Stunnenberg, {Hendrik G} and Stefan Karlsson and Tariq Enver",

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doi = "10.1016/j.celrep.2013.07.020",

language = "English",

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T1 - The tetraspanin CD9 affords high-purity capture of all murine hematopoietic stem cells

AU - Karlsson, Göran

AU - Rörby, Emma

AU - Pina, Cristina

AU - Soneji, Shamit

AU - Reckzeh, Kristian

AU - Miharada, Kenichi

AU - Karlsson, Christine

AU - Guo, Yanping

AU - Fugazza, Cristina

AU - Gupta, Rajeev

AU - Martens, Joost H A

AU - Stunnenberg, Hendrik G

AU - Karlsson, Stefan

AU - Enver, Tariq

PY - 2013/8/29

Y1 - 2013/8/29

N2 - Prospective isolation is critical for understanding the cellular and molecular aspects of stem cell heterogeneity. Here, we identify the cell surface antigen CD9 as a positive marker that provides a simple alternative for hematopoietic stem cell isolation at high purity. Crucially, CD9 affords the capture of all hematopoietic stem cells in murine bone marrow in the absence of contaminating populations that lack authentic stem cell function. Using CD9 as a tool to subdivide hematopoietic stem-cell-containing populations, we provide evidence for heterogeneity at the cellular, functional, and molecular levels.

AB - Prospective isolation is critical for understanding the cellular and molecular aspects of stem cell heterogeneity. Here, we identify the cell surface antigen CD9 as a positive marker that provides a simple alternative for hematopoietic stem cell isolation at high purity. Crucially, CD9 affords the capture of all hematopoietic stem cells in murine bone marrow in the absence of contaminating populations that lack authentic stem cell function. Using CD9 as a tool to subdivide hematopoietic stem-cell-containing populations, we provide evidence for heterogeneity at the cellular, functional, and molecular levels.

KW - Animals

KW - Biomarkers/analysis

KW - Cell Separation/methods

KW - Flow Cytometry/methods

KW - Hematopoietic Stem Cell Transplantation

KW - Hematopoietic Stem Cells/chemistry

KW - Mice

KW - Mice, Inbred C57BL

KW - Tetraspanin-29/analysis

U2 - 10.1016/j.celrep.2013.07.020

DO - 10.1016/j.celrep.2013.07.020

M3 - Journal article

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SP - 642

EP - 648

JO - Cell Reports

JF - Cell Reports

IS - 4

ER -

The tetraspanin CD9 affords high-purity capture of all murine hematopoietic stem cells

Abstract

Keywords

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