The tetraspanin CD9 affords high-purity capture of all murine hematopoietic stem cells

Göran Karlsson, Emma Rörby, Cristina Pina, Shamit Soneji, Kristian Reckzeh, Kenichi Miharada, Christine Karlsson, Yanping Guo, Cristina Fugazza, Rajeev Gupta, Joost H A Martens, Hendrik G Stunnenberg, Stefan Karlsson, Tariq Enver

27 Citationer (Scopus)

Abstract

Prospective isolation is critical for understanding the cellular and molecular aspects of stem cell heterogeneity. Here, we identify the cell surface antigen CD9 as a positive marker that provides a simple alternative for hematopoietic stem cell isolation at high purity. Crucially, CD9 affords the capture of all hematopoietic stem cells in murine bone marrow in the absence of contaminating populations that lack authentic stem cell function. Using CD9 as a tool to subdivide hematopoietic stem-cell-containing populations, we provide evidence for heterogeneity at the cellular, functional, and molecular levels.

OriginalsprogEngelsk
TidsskriftCell Reports
Vol/bind4
Udgave nummer4
Sider (fra-til)642-8
Antal sider7
ISSN2211-1247
DOI
StatusUdgivet - 29 aug. 2013
Udgivet eksterntJa

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