The structurally effect of surface coated rhamnogalacturonan I on response of the osteoblast-like cell line SaOS-2

Rikke Svava; Katarzyna Gurzawska; Yu Yihau; Kenneth Brian Haugshøj; Kai Dirscherl; Steven Bruce Levery; Niklas Rye Jørgensen; Klaus Gotfredsen; Iben Damager; Peter Ulvskov; Bodil Jørgensen

doi:10.1002/jbm.a.34868

The structurally effect of surface coated rhamnogalacturonan I on response of the osteoblast-like cell line SaOS-2

Rikke Svava, Katarzyna Gurzawska, Yu Yihau, Kenneth Brian Haugshøj, Kai Dirscherl, Steven Bruce Levery, Niklas Rye Jørgensen, Klaus Gotfredsen, Iben Damager, Peter Ulvskov, Bodil Jørgensen

5 Citations (Scopus)

Abstract

Osseointegration is important when implants are inserted into the bone and can be improved by biochemical surface coating of the implant. In this paper enzymatically modified rhamnogalacturonan I (RG-I) from apple and lupin was used for biochemical coating of aminated surfaces and the importance of the quality of RG-I, the nature of the binding, the fine structure of RG-I, and its effect on SaOS-2 cell line cultured on coated surfaces was investigated. SaOS-2 cells are osteoblast-like cells and a well-established in vitro model of bone-matrix forming osteoblasts. Purification by gel filtration could remove small fragments of galacturonic acid (GalA) and binding studies showed that the purity of the RG-I molecules was important for the quality of the coating. The structure of RG-I and osteoblast-like cells' viability were positively correlated so that high content of 1,4-linked galactose (Gal) and a low content of arabinose in the RG-I molecules favored cell viability. These results indicate that coating of implants with RG-I affect osseointegration positively.

Original language	English
Journal	Journal of Biomedical Materials Research. Part A
Volume	102
Issue number	6
Pages (from-to)	1961-1971
Number of pages	11
ISSN	1549-3296
DOIs	https://doi.org/10.1002/jbm.a.34868
Publication status	Published - Jun 2014

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Svava, R., Gurzawska, K., Yihau, Y., Haugshøj, K. B., Dirscherl, K., Levery, S. B., Jørgensen, N. R., Gotfredsen, K., Damager, I., Ulvskov, P., & Jørgensen, B. (2014). The structurally effect of surface coated rhamnogalacturonan I on response of the osteoblast-like cell line SaOS-2. Journal of Biomedical Materials Research. Part A, 102(6), 1961-1971. https://doi.org/10.1002/jbm.a.34868

Svava, R, Gurzawska, K, Yihau, Y, Haugshøj, KB, Dirscherl, K, Levery, SB, Jørgensen, NR , Gotfredsen, K, Damager, I, Ulvskov, P & Jørgensen, B 2014, 'The structurally effect of surface coated rhamnogalacturonan I on response of the osteoblast-like cell line SaOS-2', Journal of Biomedical Materials Research. Part A, vol. 102, no. 6, pp. 1961-1971. https://doi.org/10.1002/jbm.a.34868

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title = "The structurally effect of surface coated rhamnogalacturonan I on response of the osteoblast-like cell line SaOS-2",

abstract = "Osseointegration is important when implants are inserted into the bone and can be improved by biochemical surface coating of the implant. In this paper enzymatically modified rhamnogalacturonan I (RG-I) from apple and lupin was used for biochemical coating of aminated surfaces and the importance of the quality of RG-I, the nature of the binding, the fine structure of RG-I, and its effect on SaOS-2 cell line cultured on coated surfaces was investigated. SaOS-2 cells are osteoblast-like cells and a well-established in vitro model of bone-matrix forming osteoblasts. Purification by gel filtration could remove small fragments of galacturonic acid (GalA) and binding studies showed that the purity of the RG-I molecules was important for the quality of the coating. The structure of RG-I and osteoblast-like cells' viability were positively correlated so that high content of 1,4-linked galactose (Gal) and a low content of arabinose in the RG-I molecules favored cell viability. These results indicate that coating of implants with RG-I affect osseointegration positively.",

author = "Rikke Svava and Katarzyna Gurzawska and Yu Yihau and Haugsh{\o}j, {Kenneth Brian} and Kai Dirscherl and Levery, {Steven Bruce} and J{\o}rgensen, {Niklas Rye} and Klaus Gotfredsen and Iben Damager and Peter Ulvskov and Bodil J{\o}rgensen",

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AU - Svava, Rikke

AU - Gurzawska, Katarzyna

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AU - Haugshøj, Kenneth Brian

AU - Dirscherl, Kai

AU - Levery, Steven Bruce

AU - Jørgensen, Niklas Rye

AU - Gotfredsen, Klaus

AU - Damager, Iben

AU - Ulvskov, Peter

AU - Jørgensen, Bodil

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N2 - Osseointegration is important when implants are inserted into the bone and can be improved by biochemical surface coating of the implant. In this paper enzymatically modified rhamnogalacturonan I (RG-I) from apple and lupin was used for biochemical coating of aminated surfaces and the importance of the quality of RG-I, the nature of the binding, the fine structure of RG-I, and its effect on SaOS-2 cell line cultured on coated surfaces was investigated. SaOS-2 cells are osteoblast-like cells and a well-established in vitro model of bone-matrix forming osteoblasts. Purification by gel filtration could remove small fragments of galacturonic acid (GalA) and binding studies showed that the purity of the RG-I molecules was important for the quality of the coating. The structure of RG-I and osteoblast-like cells' viability were positively correlated so that high content of 1,4-linked galactose (Gal) and a low content of arabinose in the RG-I molecules favored cell viability. These results indicate that coating of implants with RG-I affect osseointegration positively.

AB - Osseointegration is important when implants are inserted into the bone and can be improved by biochemical surface coating of the implant. In this paper enzymatically modified rhamnogalacturonan I (RG-I) from apple and lupin was used for biochemical coating of aminated surfaces and the importance of the quality of RG-I, the nature of the binding, the fine structure of RG-I, and its effect on SaOS-2 cell line cultured on coated surfaces was investigated. SaOS-2 cells are osteoblast-like cells and a well-established in vitro model of bone-matrix forming osteoblasts. Purification by gel filtration could remove small fragments of galacturonic acid (GalA) and binding studies showed that the purity of the RG-I molecules was important for the quality of the coating. The structure of RG-I and osteoblast-like cells' viability were positively correlated so that high content of 1,4-linked galactose (Gal) and a low content of arabinose in the RG-I molecules favored cell viability. These results indicate that coating of implants with RG-I affect osseointegration positively.

U2 - 10.1002/jbm.a.34868

DO - 10.1002/jbm.a.34868

M3 - Journal article

C2 - 23852647

SN - 1549-3296

VL - 102

SP - 1961

EP - 1971

JO - Journal of Biomedical Materials Research. Part A

JF - Journal of Biomedical Materials Research. Part A

IS - 6

ER -

The structurally effect of surface coated rhamnogalacturonan I on response of the osteoblast-like cell line SaOS-2

Abstract

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