TY - JOUR
T1 - The structurally effect of surface coated rhamnogalacturonan I on response of the osteoblast-like cell line SaOS-2
AU - Svava, Rikke
AU - Gurzawska, Katarzyna
AU - Yihau, Yu
AU - Haugshøj, Kenneth Brian
AU - Dirscherl, Kai
AU - Levery, Steven Bruce
AU - Jørgensen, Niklas Rye
AU - Gotfredsen, Klaus
AU - Damager, Iben
AU - Ulvskov, Peter
AU - Jørgensen, Bodil
N1 - Copyright © 2013 Wiley Periodicals, Inc., A Wiley Company.
PY - 2014/6
Y1 - 2014/6
N2 - Osseointegration is important when implants are inserted into the bone and can be improved by biochemical surface coating of the implant. In this paper enzymatically modified rhamnogalacturonan I (RG-I) from apple and lupin was used for biochemical coating of aminated surfaces and the importance of the quality of RG-I, the nature of the binding, the fine structure of RG-I, and its effect on SaOS-2 cell line cultured on coated surfaces was investigated. SaOS-2 cells are osteoblast-like cells and a well-established in vitro model of bone-matrix forming osteoblasts. Purification by gel filtration could remove small fragments of galacturonic acid (GalA) and binding studies showed that the purity of the RG-I molecules was important for the quality of the coating. The structure of RG-I and osteoblast-like cells' viability were positively correlated so that high content of 1,4-linked galactose (Gal) and a low content of arabinose in the RG-I molecules favored cell viability. These results indicate that coating of implants with RG-I affect osseointegration positively.
AB - Osseointegration is important when implants are inserted into the bone and can be improved by biochemical surface coating of the implant. In this paper enzymatically modified rhamnogalacturonan I (RG-I) from apple and lupin was used for biochemical coating of aminated surfaces and the importance of the quality of RG-I, the nature of the binding, the fine structure of RG-I, and its effect on SaOS-2 cell line cultured on coated surfaces was investigated. SaOS-2 cells are osteoblast-like cells and a well-established in vitro model of bone-matrix forming osteoblasts. Purification by gel filtration could remove small fragments of galacturonic acid (GalA) and binding studies showed that the purity of the RG-I molecules was important for the quality of the coating. The structure of RG-I and osteoblast-like cells' viability were positively correlated so that high content of 1,4-linked galactose (Gal) and a low content of arabinose in the RG-I molecules favored cell viability. These results indicate that coating of implants with RG-I affect osseointegration positively.
U2 - 10.1002/jbm.a.34868
DO - 10.1002/jbm.a.34868
M3 - Journal article
C2 - 23852647
SN - 1549-3296
VL - 102
SP - 1961
EP - 1971
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
IS - 6
ER -