Abstract
In the fission yeast Schizosaccharomyces pombe, cells of opposite mating type communicate via diffusible peptide pheromones prior to mating. We have cloned the S. pombe mam1 gene, which encodes a 1336-amino acid protein belonging to the ATP-binding cassette (ABC) superfamily. The mam1 gene is only expressed in M cells and the gene product is responsible for the secretion of the mating pheromone. M-factor, a nonapeptide that is S-farnesylated and carboxy-methylated on its C-terminal cysteine residue. The predicted Mam1 protein is highly homologous to mammalian multiple drug-resistance proteins and to the Saccharomyces cerevisiae STE6 gene product, which mediates export of a-factor mating pheromone. We show that STE6 can also mediate secretion of M-factor in S. pombe.
| Original language | English |
|---|---|
| Journal | Molecular and General Genetics MGG |
| Volume | 255 |
| Issue number | 2 |
| Pages (from-to) | 226-36 |
| Number of pages | 11 |
| ISSN | 0026-8925 |
| Publication status | Published - 1 Jun 1997 |
Keywords
- ATP-Binding Cassette Transporters
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Fungal Proteins
- Gene Expression Regulation, Fungal
- Genetic Complementation Test
- Glycoproteins
- Guanine Nucleotide Exchange Factors
- Molecular Sequence Data
- Mutation
- Nitrogen
- P-Glycoprotein
- Peptides
- Pheromones
- Saccharomyces cerevisiae Proteins
- Schizosaccharomyces
- Schizosaccharomyces pombe Proteins
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Transcription, Genetic