The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor

P U Christensen; William John Davey; O Nielsen; Olaf Nielsen

The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor

P U Christensen, William John Davey, O Nielsen, Olaf Nielsen

Functional genomics

55 Citationer (Scopus)

Abstract

In the fission yeast Schizosaccharomyces pombe, cells of opposite mating type communicate via diffusible peptide pheromones prior to mating. We have cloned the S. pombe mam1 gene, which encodes a 1336-amino acid protein belonging to the ATP-binding cassette (ABC) superfamily. The mam1 gene is only expressed in M cells and the gene product is responsible for the secretion of the mating pheromone. M-factor, a nonapeptide that is S-farnesylated and carboxy-methylated on its C-terminal cysteine residue. The predicted Mam1 protein is highly homologous to mammalian multiple drug-resistance proteins and to the Saccharomyces cerevisiae STE6 gene product, which mediates export of a-factor mating pheromone. We show that STE6 can also mediate secretion of M-factor in S. pombe.

Originalsprog	Engelsk
Tidsskrift	Molecular and General Genetics MGG
Vol/bind	255
Udgave nummer	2
Sider (fra-til)	226-36
Antal sider	11
ISSN	0026-8925
Status	Udgivet - 1 jun. 1997

Citationsformater

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title = "The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor",

abstract = "In the fission yeast Schizosaccharomyces pombe, cells of opposite mating type communicate via diffusible peptide pheromones prior to mating. We have cloned the S. pombe mam1 gene, which encodes a 1336-amino acid protein belonging to the ATP-binding cassette (ABC) superfamily. The mam1 gene is only expressed in M cells and the gene product is responsible for the secretion of the mating pheromone. M-factor, a nonapeptide that is S-farnesylated and carboxy-methylated on its C-terminal cysteine residue. The predicted Mam1 protein is highly homologous to mammalian multiple drug-resistance proteins and to the Saccharomyces cerevisiae STE6 gene product, which mediates export of a-factor mating pheromone. We show that STE6 can also mediate secretion of M-factor in S. pombe.",

keywords = "ATP-Binding Cassette Transporters, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Fungal Proteins, Gene Expression Regulation, Fungal, Genetic Complementation Test, Glycoproteins, Guanine Nucleotide Exchange Factors, Molecular Sequence Data, Mutation, Nitrogen, P-Glycoprotein, Peptides, Pheromones, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Transcription, Genetic",

author = "Christensen, {P U} and Davey, {William John} and O Nielsen and Olaf Nielsen",

year = "1997",

month = jun,

day = "1",

language = "English",

volume = "255",

pages = "226--36",

journal = "Molecular General Genetics",

issn = "0026-8925",

publisher = "Springer Verlag",

number = "2",

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TY - JOUR

T1 - The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor

AU - Christensen, P U

AU - Davey, William John

AU - Nielsen, O

AU - Nielsen, Olaf

PY - 1997/6/1

Y1 - 1997/6/1

N2 - In the fission yeast Schizosaccharomyces pombe, cells of opposite mating type communicate via diffusible peptide pheromones prior to mating. We have cloned the S. pombe mam1 gene, which encodes a 1336-amino acid protein belonging to the ATP-binding cassette (ABC) superfamily. The mam1 gene is only expressed in M cells and the gene product is responsible for the secretion of the mating pheromone. M-factor, a nonapeptide that is S-farnesylated and carboxy-methylated on its C-terminal cysteine residue. The predicted Mam1 protein is highly homologous to mammalian multiple drug-resistance proteins and to the Saccharomyces cerevisiae STE6 gene product, which mediates export of a-factor mating pheromone. We show that STE6 can also mediate secretion of M-factor in S. pombe.

AB - In the fission yeast Schizosaccharomyces pombe, cells of opposite mating type communicate via diffusible peptide pheromones prior to mating. We have cloned the S. pombe mam1 gene, which encodes a 1336-amino acid protein belonging to the ATP-binding cassette (ABC) superfamily. The mam1 gene is only expressed in M cells and the gene product is responsible for the secretion of the mating pheromone. M-factor, a nonapeptide that is S-farnesylated and carboxy-methylated on its C-terminal cysteine residue. The predicted Mam1 protein is highly homologous to mammalian multiple drug-resistance proteins and to the Saccharomyces cerevisiae STE6 gene product, which mediates export of a-factor mating pheromone. We show that STE6 can also mediate secretion of M-factor in S. pombe.

KW - ATP-Binding Cassette Transporters

KW - Amino Acid Sequence

KW - Base Sequence

KW - Cloning, Molecular

KW - Fungal Proteins

KW - Gene Expression Regulation, Fungal

KW - Genetic Complementation Test

KW - Glycoproteins

KW - Guanine Nucleotide Exchange Factors

KW - Molecular Sequence Data

KW - Mutation

KW - Nitrogen

KW - P-Glycoprotein

KW - Peptides

KW - Pheromones

KW - Saccharomyces cerevisiae Proteins

KW - Schizosaccharomyces

KW - Schizosaccharomyces pombe Proteins

KW - Sequence Analysis, DNA

KW - Sequence Homology, Amino Acid

KW - Transcription, Genetic

M3 - Journal article

C2 - 9236781

SN - 0026-8925

VL - 255

SP - 226

EP - 236

JO - Molecular General Genetics

JF - Molecular General Genetics

IS - 2

ER -

The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor

Abstract

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