The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor

TY - JOUR

T1 - The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor

AU - Christensen, P U

AU - Davey, William John

AU - Nielsen, O

AU - Nielsen, Olaf

PY - 1997/6/1

Y1 - 1997/6/1

N2 - In the fission yeast Schizosaccharomyces pombe, cells of opposite mating type communicate via diffusible peptide pheromones prior to mating. We have cloned the S. pombe mam1 gene, which encodes a 1336-amino acid protein belonging to the ATP-binding cassette (ABC) superfamily. The mam1 gene is only expressed in M cells and the gene product is responsible for the secretion of the mating pheromone. M-factor, a nonapeptide that is S-farnesylated and carboxy-methylated on its C-terminal cysteine residue. The predicted Mam1 protein is highly homologous to mammalian multiple drug-resistance proteins and to the Saccharomyces cerevisiae STE6 gene product, which mediates export of a-factor mating pheromone. We show that STE6 can also mediate secretion of M-factor in S. pombe.

AB - In the fission yeast Schizosaccharomyces pombe, cells of opposite mating type communicate via diffusible peptide pheromones prior to mating. We have cloned the S. pombe mam1 gene, which encodes a 1336-amino acid protein belonging to the ATP-binding cassette (ABC) superfamily. The mam1 gene is only expressed in M cells and the gene product is responsible for the secretion of the mating pheromone. M-factor, a nonapeptide that is S-farnesylated and carboxy-methylated on its C-terminal cysteine residue. The predicted Mam1 protein is highly homologous to mammalian multiple drug-resistance proteins and to the Saccharomyces cerevisiae STE6 gene product, which mediates export of a-factor mating pheromone. We show that STE6 can also mediate secretion of M-factor in S. pombe.

KW - ATP-Binding Cassette Transporters

KW - Amino Acid Sequence

KW - Base Sequence

KW - Cloning, Molecular

KW - Fungal Proteins

KW - Gene Expression Regulation, Fungal

KW - Genetic Complementation Test

KW - Glycoproteins

KW - Guanine Nucleotide Exchange Factors

KW - Molecular Sequence Data

KW - Mutation

KW - Nitrogen

KW - P-Glycoprotein

KW - Peptides

KW - Pheromones

KW - Saccharomyces cerevisiae Proteins

KW - Schizosaccharomyces

KW - Schizosaccharomyces pombe Proteins

KW - Sequence Analysis, DNA

KW - Sequence Homology, Amino Acid

KW - Transcription, Genetic

M3 - Journal article

C2 - 9236781

SN - 0026-8925

VL - 255

SP - 226

EP - 236

JO - Molecular and General Genetics MGG

JF - Molecular and General Genetics MGG

IS - 2

ER -