The role of miRNAs in human papilloma virus (HPV)-associated cancers: bridging between HPV-related head and neck cancer and cervical cancer

TY - JOUR

T1 - The role of miRNAs in human papilloma virus (HPV)-associated cancers

T2 - bridging between HPV-related head and neck cancer and cervical cancer

AU - Lajer, C.B.

AU - Garnæs, E.

AU - Friis-Hansen, Lennart Jan

AU - Norrild, Bodil

AU - Therkildsen, Marianne Hamilton

AU - Glud, Martin

AU - Rossing, M.

AU - Lajer, Henrik

AU - Svane, Danny

AU - Skotte, Line

AU - Specht, Lena

AU - von Buchwald, Christian

AU - Nielsen, Finn Cilius

PY - 2012/4/24

Y1 - 2012/4/24

N2 - Background: Although the role of human papilloma virus (HPV) in cervical squamous cell carcinoma (CSCC) is well established, the role in head and neck SCC (HNSCC) is less clear. MicroRNAs (miRNAs) have a role in the cancer development, and HPV status may affect the miRNA expression pattern in HNSCC. To explore the influence of HPV in HNSCC, we made a comparative miRNA profile of HPV-positive (HPV) and HPV-negative (HPV) HNSCC against CSCC. Methods: Fresh frozen and laser microdissected-paraffin-embedded samples obtained from patients with HPV/HPV HNSCC, CSCC and controls were used for microarray analysis. Differentially expressed miRNAs in the HPV and HPV HNSCC samples were compared with the differentially expressed miRNAs in the CSCC samples.Results:Human papilloma virus positive () HNSCC had a distinct miRNA profile compared with HPV HNSCC. Significantly more similarity was seen between HPV HNSCC and CSCC than HPV and CSCC. A set of HPV core miRNAs were identified. Of these especially the miR-15a/miR-16/miR195/miR-497 family, miR-143/miR-145 and the miR-106-363 cluster appear to be important within the known HPV pathogenesis. Conclusion: This study adds new knowledge to the known pathogenic pathways of HPV and substantiates the oncogenic role of HPV in subsets of HNSCCs.

AB - Background: Although the role of human papilloma virus (HPV) in cervical squamous cell carcinoma (CSCC) is well established, the role in head and neck SCC (HNSCC) is less clear. MicroRNAs (miRNAs) have a role in the cancer development, and HPV status may affect the miRNA expression pattern in HNSCC. To explore the influence of HPV in HNSCC, we made a comparative miRNA profile of HPV-positive (HPV) and HPV-negative (HPV) HNSCC against CSCC. Methods: Fresh frozen and laser microdissected-paraffin-embedded samples obtained from patients with HPV/HPV HNSCC, CSCC and controls were used for microarray analysis. Differentially expressed miRNAs in the HPV and HPV HNSCC samples were compared with the differentially expressed miRNAs in the CSCC samples.Results:Human papilloma virus positive () HNSCC had a distinct miRNA profile compared with HPV HNSCC. Significantly more similarity was seen between HPV HNSCC and CSCC than HPV and CSCC. A set of HPV core miRNAs were identified. Of these especially the miR-15a/miR-16/miR195/miR-497 family, miR-143/miR-145 and the miR-106-363 cluster appear to be important within the known HPV pathogenesis. Conclusion: This study adds new knowledge to the known pathogenic pathways of HPV and substantiates the oncogenic role of HPV in subsets of HNSCCs.

KW - Adolescent

KW - Carcinoma, Squamous Cell

KW - Case-Control Studies

KW - Child

KW - DNA, Viral

KW - Female

KW - Gene Expression Profiling

KW - Head and Neck Neoplasms

KW - Humans

KW - Laser Capture Microdissection

KW - Male

KW - MicroRNAs

KW - Middle Aged

KW - Neoplasm Staging

KW - Oligonucleotide Array Sequence Analysis

KW - Papillomaviridae

KW - Papillomavirus Infections

KW - Prognosis

KW - RNA, Messenger

KW - Real-Time Polymerase Chain Reaction

KW - Tumor Markers, Biological

U2 - 10.1038/bjc.2017.203

DO - 10.1038/bjc.2017.203

M3 - Journal article

C2 - 22472886

SN - 0007-0920

VL - 106

SP - 1526

EP - 1534

JO - B J C

JF - B J C

ER -