TY - JOUR
T1 - The role of miRNAs in human papilloma virus (HPV)-associated cancers
T2 - bridging between HPV-related head and neck cancer and cervical cancer
AU - Lajer, C.B.
AU - Garnæs, E.
AU - Friis-Hansen, Lennart Jan
AU - Norrild, Bodil
AU - Therkildsen, Marianne Hamilton
AU - Glud, Martin
AU - Rossing, M.
AU - Lajer, Henrik
AU - Svane, Danny
AU - Skotte, Line
AU - Specht, Lena
AU - von Buchwald, Christian
AU - Nielsen, Finn Cilius
PY - 2012/4/24
Y1 - 2012/4/24
N2 - Background: Although the role of human papilloma virus (HPV) in cervical squamous cell carcinoma (CSCC) is well established, the role in head and neck SCC (HNSCC) is less clear. MicroRNAs (miRNAs) have a role in the cancer development, and HPV status may affect the miRNA expression pattern in HNSCC. To explore the influence of HPV in HNSCC, we made a comparative miRNA profile of HPV-positive (HPV) and HPV-negative (HPV) HNSCC against CSCC. Methods: Fresh frozen and laser microdissected-paraffin-embedded samples obtained from patients with HPV/HPV HNSCC, CSCC and controls were used for microarray analysis. Differentially expressed miRNAs in the HPV and HPV HNSCC samples were compared with the differentially expressed miRNAs in the CSCC samples.Results:Human papilloma virus positive () HNSCC had a distinct miRNA profile compared with HPV HNSCC. Significantly more similarity was seen between HPV HNSCC and CSCC than HPV and CSCC. A set of HPV core miRNAs were identified. Of these especially the miR-15a/miR-16/miR195/miR-497 family, miR-143/miR-145 and the miR-106-363 cluster appear to be important within the known HPV pathogenesis. Conclusion: This study adds new knowledge to the known pathogenic pathways of HPV and substantiates the oncogenic role of HPV in subsets of HNSCCs.
AB - Background: Although the role of human papilloma virus (HPV) in cervical squamous cell carcinoma (CSCC) is well established, the role in head and neck SCC (HNSCC) is less clear. MicroRNAs (miRNAs) have a role in the cancer development, and HPV status may affect the miRNA expression pattern in HNSCC. To explore the influence of HPV in HNSCC, we made a comparative miRNA profile of HPV-positive (HPV) and HPV-negative (HPV) HNSCC against CSCC. Methods: Fresh frozen and laser microdissected-paraffin-embedded samples obtained from patients with HPV/HPV HNSCC, CSCC and controls were used for microarray analysis. Differentially expressed miRNAs in the HPV and HPV HNSCC samples were compared with the differentially expressed miRNAs in the CSCC samples.Results:Human papilloma virus positive () HNSCC had a distinct miRNA profile compared with HPV HNSCC. Significantly more similarity was seen between HPV HNSCC and CSCC than HPV and CSCC. A set of HPV core miRNAs were identified. Of these especially the miR-15a/miR-16/miR195/miR-497 family, miR-143/miR-145 and the miR-106-363 cluster appear to be important within the known HPV pathogenesis. Conclusion: This study adds new knowledge to the known pathogenic pathways of HPV and substantiates the oncogenic role of HPV in subsets of HNSCCs.
KW - Adolescent
KW - Carcinoma, Squamous Cell
KW - Case-Control Studies
KW - Child
KW - DNA, Viral
KW - Female
KW - Gene Expression Profiling
KW - Head and Neck Neoplasms
KW - Humans
KW - Laser Capture Microdissection
KW - Male
KW - MicroRNAs
KW - Middle Aged
KW - Neoplasm Staging
KW - Oligonucleotide Array Sequence Analysis
KW - Papillomaviridae
KW - Papillomavirus Infections
KW - Prognosis
KW - RNA, Messenger
KW - Real-Time Polymerase Chain Reaction
KW - Tumor Markers, Biological
U2 - 10.1038/bjc.2017.203
DO - 10.1038/bjc.2017.203
M3 - Journal article
C2 - 22472886
SN - 0007-0920
VL - 106
SP - 1526
EP - 1534
JO - The British journal of cancer. Supplement
JF - The British journal of cancer. Supplement
ER -
