The Role of Endoglucanase and Endoxylanase in Liquefaction of Hydrothermally Pretreated Wheat Straw

Pernille Anastasia Skovgaard; Lisbeth Garbrecht Thygesen; Henning Jørgensen; Maria Cardona; Emilio Tozzi; Michael McCarthy; Matti  Siika-Aho; Tina  Jeoh

doi:10.1002/btpr.1893

The Role of Endoglucanase and Endoxylanase in Liquefaction of Hydrothermally Pretreated Wheat Straw

Pernille Anastasia Skovgaard, Lisbeth Garbrecht Thygesen, Henning Jørgensen, Maria Cardona, Emilio Tozzi, Michael McCarthy, Matti Siika-Aho, Tina Jeoh

Forest, Nature and Biomass

18 Citations (Scopus)

Abstract

The role of endocellulases and endoxylanase during liquefaction and saccharification of hydrothermally pretreated wheat straw was studied. The use of a flow-loop setup with in-line magnetic resonance imaging enabled frequent measurements of viscosity at 55°C during saccharification at 6% total solids content. Viscosity data were complemented with off-line measurements of fiber lengths and release of soluble sugars. A clear correlation between fiber attrition and a decrease in viscosity was found. Fiber lengths and viscosity dropped quickly within the first hour and then stagnated, while sugar yields increased substantially thereafter, illustrating that liquefaction and saccharification are separate mechanisms. Both endoglucanase and endoxylanase were shown to have a significant effect on viscosity during liquefaction while the addition of endoxylanase also increased sugar yield.

Original language	English
Journal	Biotechnology Progress
Volume	30
Issue number	4
Pages (from-to)	923-931
Number of pages	9
ISSN	8756-7938
DOIs	https://doi.org/10.1002/btpr.1893
Publication status	Published - 2014

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title = "The Role of Endoglucanase and Endoxylanase in Liquefaction of Hydrothermally Pretreated Wheat Straw",

abstract = "The role of endocellulases and endoxylanase during liquefaction and saccharification of hydrothermally pretreated wheat straw was studied. The use of a flow-loop setup with in-line magnetic resonance imaging enabled frequent measurements of viscosity at 55°C during saccharification at 6% total solids content. Viscosity data were complemented with off-line measurements of fiber lengths and release of soluble sugars. A clear correlation between fiber attrition and a decrease in viscosity was found. Fiber lengths and viscosity dropped quickly within the first hour and then stagnated, while sugar yields increased substantially thereafter, illustrating that liquefaction and saccharification are separate mechanisms. Both endoglucanase and endoxylanase were shown to have a significant effect on viscosity during liquefaction while the addition of endoxylanase also increased sugar yield.",

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T1 - The Role of Endoglucanase and Endoxylanase in Liquefaction of Hydrothermally Pretreated Wheat Straw

AU - Skovgaard, Pernille Anastasia

AU - Thygesen, Lisbeth Garbrecht

AU - Jørgensen, Henning

AU - Cardona, Maria

AU - Tozzi, Emilio

AU - McCarthy, Michael

AU - Siika-Aho, Matti

AU - Jeoh, Tina

PY - 2014

Y1 - 2014

N2 - The role of endocellulases and endoxylanase during liquefaction and saccharification of hydrothermally pretreated wheat straw was studied. The use of a flow-loop setup with in-line magnetic resonance imaging enabled frequent measurements of viscosity at 55°C during saccharification at 6% total solids content. Viscosity data were complemented with off-line measurements of fiber lengths and release of soluble sugars. A clear correlation between fiber attrition and a decrease in viscosity was found. Fiber lengths and viscosity dropped quickly within the first hour and then stagnated, while sugar yields increased substantially thereafter, illustrating that liquefaction and saccharification are separate mechanisms. Both endoglucanase and endoxylanase were shown to have a significant effect on viscosity during liquefaction while the addition of endoxylanase also increased sugar yield.

AB - The role of endocellulases and endoxylanase during liquefaction and saccharification of hydrothermally pretreated wheat straw was studied. The use of a flow-loop setup with in-line magnetic resonance imaging enabled frequent measurements of viscosity at 55°C during saccharification at 6% total solids content. Viscosity data were complemented with off-line measurements of fiber lengths and release of soluble sugars. A clear correlation between fiber attrition and a decrease in viscosity was found. Fiber lengths and viscosity dropped quickly within the first hour and then stagnated, while sugar yields increased substantially thereafter, illustrating that liquefaction and saccharification are separate mechanisms. Both endoglucanase and endoxylanase were shown to have a significant effect on viscosity during liquefaction while the addition of endoxylanase also increased sugar yield.

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DO - 10.1002/btpr.1893

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The Role of Endoglucanase and Endoxylanase in Liquefaction of Hydrothermally Pretreated Wheat Straw

Abstract

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