The Role of Endoglucanase and Endoxylanase in Liquefaction of Hydrothermally Pretreated Wheat Straw

Pernille Anastasia Skovgaard; Lisbeth Garbrecht Thygesen; Henning Jørgensen; Maria Cardona; Emilio Tozzi; Michael McCarthy; Matti  Siika-Aho; Tina  Jeoh

doi:10.1002/btpr.1893

The Role of Endoglucanase and Endoxylanase in Liquefaction of Hydrothermally Pretreated Wheat Straw

Pernille Anastasia Skovgaard, Lisbeth Garbrecht Thygesen, Henning Jørgensen, Maria Cardona, Emilio Tozzi, Michael McCarthy, Matti Siika-Aho, Tina Jeoh

LUKKET: Skov, natur og biomasse

18 Citationer (Scopus)

Abstract

The role of endocellulases and endoxylanase during liquefaction and saccharification of hydrothermally pretreated wheat straw was studied. The use of a flow-loop setup with in-line magnetic resonance imaging enabled frequent measurements of viscosity at 55°C during saccharification at 6% total solids content. Viscosity data were complemented with off-line measurements of fiber lengths and release of soluble sugars. A clear correlation between fiber attrition and a decrease in viscosity was found. Fiber lengths and viscosity dropped quickly within the first hour and then stagnated, while sugar yields increased substantially thereafter, illustrating that liquefaction and saccharification are separate mechanisms. Both endoglucanase and endoxylanase were shown to have a significant effect on viscosity during liquefaction while the addition of endoxylanase also increased sugar yield.

Originalsprog	Engelsk
Tidsskrift	Biotechnology Progress
Vol/bind	30
Udgave nummer	4
Sider (fra-til)	923-931
Antal sider	9
ISSN	8756-7938
DOI	https://doi.org/10.1002/btpr.1893
Status	Udgivet - 2014

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10.1002/btpr.1893

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Citationsformater

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abstract = "The role of endocellulases and endoxylanase during liquefaction and saccharification of hydrothermally pretreated wheat straw was studied. The use of a flow-loop setup with in-line magnetic resonance imaging enabled frequent measurements of viscosity at 55°C during saccharification at 6% total solids content. Viscosity data were complemented with off-line measurements of fiber lengths and release of soluble sugars. A clear correlation between fiber attrition and a decrease in viscosity was found. Fiber lengths and viscosity dropped quickly within the first hour and then stagnated, while sugar yields increased substantially thereafter, illustrating that liquefaction and saccharification are separate mechanisms. Both endoglucanase and endoxylanase were shown to have a significant effect on viscosity during liquefaction while the addition of endoxylanase also increased sugar yield.",

author = "Skovgaard, {Pernille Anastasia} and Thygesen, {Lisbeth Garbrecht} and Henning J{\o}rgensen and Maria Cardona and Emilio Tozzi and Michael McCarthy and Matti Siika-Aho and Tina Jeoh",

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T1 - The Role of Endoglucanase and Endoxylanase in Liquefaction of Hydrothermally Pretreated Wheat Straw

AU - Skovgaard, Pernille Anastasia

AU - Thygesen, Lisbeth Garbrecht

AU - Jørgensen, Henning

AU - Cardona, Maria

AU - Tozzi, Emilio

AU - McCarthy, Michael

AU - Siika-Aho, Matti

AU - Jeoh, Tina

PY - 2014

Y1 - 2014

N2 - The role of endocellulases and endoxylanase during liquefaction and saccharification of hydrothermally pretreated wheat straw was studied. The use of a flow-loop setup with in-line magnetic resonance imaging enabled frequent measurements of viscosity at 55°C during saccharification at 6% total solids content. Viscosity data were complemented with off-line measurements of fiber lengths and release of soluble sugars. A clear correlation between fiber attrition and a decrease in viscosity was found. Fiber lengths and viscosity dropped quickly within the first hour and then stagnated, while sugar yields increased substantially thereafter, illustrating that liquefaction and saccharification are separate mechanisms. Both endoglucanase and endoxylanase were shown to have a significant effect on viscosity during liquefaction while the addition of endoxylanase also increased sugar yield.

AB - The role of endocellulases and endoxylanase during liquefaction and saccharification of hydrothermally pretreated wheat straw was studied. The use of a flow-loop setup with in-line magnetic resonance imaging enabled frequent measurements of viscosity at 55°C during saccharification at 6% total solids content. Viscosity data were complemented with off-line measurements of fiber lengths and release of soluble sugars. A clear correlation between fiber attrition and a decrease in viscosity was found. Fiber lengths and viscosity dropped quickly within the first hour and then stagnated, while sugar yields increased substantially thereafter, illustrating that liquefaction and saccharification are separate mechanisms. Both endoglucanase and endoxylanase were shown to have a significant effect on viscosity during liquefaction while the addition of endoxylanase also increased sugar yield.

U2 - 10.1002/btpr.1893

DO - 10.1002/btpr.1893

M3 - Journal article

C2 - 24610675

SN - 8756-7938

VL - 30

SP - 923

EP - 931

JO - Biotechnology Progress

JF - Biotechnology Progress

IS - 4

ER -

The Role of Endoglucanase and Endoxylanase in Liquefaction of Hydrothermally Pretreated Wheat Straw

Abstract

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