The PEF family proteins sorcin and grancalcin interact in vivo and in vitro.

Christian Hansen; Svetlana Tarabykina; Jonas Marstrand la Cour; Karsten Lollike; Martin W Berchtold

doi:10.1016/S0014-5793(03)00518-0

The PEF family proteins sorcin and grancalcin interact in vivo and in vitro.

Christian Hansen, Svetlana Tarabykina, Jonas Marstrand la Cour, Karsten Lollike, Martin W Berchtold

Cell Biology and Physiology

19 Citations (Scopus)

Abstract

The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.

Original language	English
Journal	FEBS Letters
Volume	545
Issue number	2-3
Pages (from-to)	151-4
Number of pages	3
ISSN	0014-5793
DOIs	https://doi.org/10.1016/S0014-5793(03)00518-0
Publication status	Published - 2003

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@article{fbcb9b60f0f911dcbee902004c4f4f50,

title = "The PEF family proteins sorcin and grancalcin interact in vivo and in vitro.",

abstract = "The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.",

author = "Christian Hansen and Svetlana Tarabykina and {la Cour}, {Jonas Marstrand} and Karsten Lollike and Berchtold, {Martin W}",

note = "Keywords: Calcium-Binding Proteins; Cells, Cultured; Dimerization; EF Hand Motifs; Endothelium, Vascular; Glutathione Transferase; Humans; Precipitin Tests; Recombinant Fusion Proteins; Two-Hybrid System Techniques; Umbilical Veins",

year = "2003",

doi = "10.1016/S0014-5793(03)00518-0",

language = "English",

volume = "545",

pages = "151--4",

journal = "F E B S Letters",

issn = "0014-5793",

publisher = "JohnWiley & Sons Ltd",

number = "2-3",

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TY - JOUR

T1 - The PEF family proteins sorcin and grancalcin interact in vivo and in vitro.

AU - Hansen, Christian

AU - Tarabykina, Svetlana

AU - la Cour, Jonas Marstrand

AU - Lollike, Karsten

AU - Berchtold, Martin W

N1 - Keywords: Calcium-Binding Proteins; Cells, Cultured; Dimerization; EF Hand Motifs; Endothelium, Vascular; Glutathione Transferase; Humans; Precipitin Tests; Recombinant Fusion Proteins; Two-Hybrid System Techniques; Umbilical Veins

PY - 2003

Y1 - 2003

N2 - The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.

AB - The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.

U2 - 10.1016/S0014-5793(03)00518-0

DO - 10.1016/S0014-5793(03)00518-0

M3 - Journal article

C2 - 12804766

SN - 0014-5793

VL - 545

SP - 151

EP - 154

JO - F E B S Letters

JF - F E B S Letters

IS - 2-3

ER -

The PEF family proteins sorcin and grancalcin interact in vivo and in vitro.

Abstract

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