@article{fbcb9b60f0f911dcbee902004c4f4f50,
title = "The PEF family proteins sorcin and grancalcin interact in vivo and in vitro.",
abstract = "The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.",
author = "Christian Hansen and Svetlana Tarabykina and {la Cour}, {Jonas Marstrand} and Karsten Lollike and Berchtold, {Martin W}",
note = "Keywords: Calcium-Binding Proteins; Cells, Cultured; Dimerization; EF Hand Motifs; Endothelium, Vascular; Glutathione Transferase; Humans; Precipitin Tests; Recombinant Fusion Proteins; Two-Hybrid System Techniques; Umbilical Veins",
year = "2003",
doi = "10.1016/S0014-5793(03)00518-0",
language = "English",
volume = "545",
pages = "151--4",
journal = "F E B S Letters",
issn = "0014-5793",
publisher = "JohnWiley & Sons Ltd",
number = "2-3",
}