The effect of whole genome amplification on samples originating from more than one donor

C.R. Thacker; M.K. Balogh; Claus Børsting; E. Ramos; Diz P. Sanchez; A. Carracedo; N. Morling; Peter M. Schneider; Denise Syndercombe-Court; SNPforID Consortium

doi:doi:10.1016/j.ics.2005.09.170

The effect of whole genome amplification on samples originating from more than one donor

C.R. Thacker, M.K. Balogh, Claus Børsting, E. Ramos, Diz P. Sanchez, A. Carracedo, N. Morling, Peter M. Schneider, Denise Syndercombe-Court, SNPforID Consortium

Section of Forensic Genetics

1 Citation (Scopus)

Abstract

In this study, the GenomiPhi(TM) DNA Amplification Kit (Amersham Biosciences) was used to investigate the potential of whole genome amplification (WGA) when considering samples originating from more than one donor. DNA was extracted from blood samples, quantified and normalised before being mixed in ratios of 1:1, 1:3, 1:7 and 1:15. Proportions were checked using standard STR analysis before being subjected to WGA. Once amplified using GenomiPhi(TM), the ratios were again checked. Relative proportions were found to be maintained in the 1:1 and 1:3 ratios following WGA; the observed peak ratios were found to match the expected peak ratios regardless of the starting concentration of DNA. With samples mixed in the ratio of 1:7 and 1:15, and when the concentration of starting material was at the manufacturer's lower limit, too few minor component peaks were found to allow for statistical analysis. With an initial template exceeding 1 ng/[mu]L there was an increase in problems associated with profile interpretation but the results obtained indicated that mixture proportions could be quantifiably maintained

Original language	English
Title of host publication	Progress in Forensic Genetics 11 : Proceedings of the 21st International ISFG Congress
Number of pages	2
Volume	1288
Publisher	Elsevier
Publication date	2006
Pages	722-724
DOIs	https://doi.org/doi:10.1016/j.ics.2005.09.170
Publication status	Published - 2006
Event	Progress in Forensic Genetics 11 - Proceedings of the 21st International ISFG Congress - Ponta Delgada, The Azores, Portugal Duration: 13 Sept 2005 → 16 Sept 2005

Conference

Conference	Progress in Forensic Genetics 11 - Proceedings of the 21st International ISFG Congress
Country/Territory	Portugal
City	Ponta Delgada, The Azores
Period	13/09/2005 → 16/09/2005

Series	ICS - International Congress Series

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doi:10.1016/j.ics.2005.09.170

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Thacker, C. R., Balogh, M. K., Børsting, C., Ramos, E., Sanchez, D. P., Carracedo, A., Morling, N., Schneider, P. M., Syndercombe-Court, D., & SNPforID Consortium (2006). The effect of whole genome amplification on samples originating from more than one donor. In Progress in Forensic Genetics 11: Proceedings of the 21st International ISFG Congress (Vol. 1288, pp. 722-724). Elsevier. ICS - International Congress Series https://doi.org/doi:10.1016/j.ics.2005.09.170

The effect of whole genome amplification on samples originating from more than one donor. / Thacker, C.R.; Balogh, M.K.; Børsting, Claus et al.

Progress in Forensic Genetics 11: Proceedings of the 21st International ISFG Congress. Vol. 1288 Elsevier, 2006. p. 722-724 (ICS - International Congress Series).

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Thacker, CR, Balogh, MK, Børsting, C, Ramos, E, Sanchez, DP, Carracedo, A, Morling, N, Schneider, PM, Syndercombe-Court, D & SNPforID Consortium 2006, The effect of whole genome amplification on samples originating from more than one donor. in Progress in Forensic Genetics 11: Proceedings of the 21st International ISFG Congress. vol. 1288, Elsevier, ICS - International Congress Series, pp. 722-724, Progress in Forensic Genetics 11 - Proceedings of the 21st International ISFG Congress, Ponta Delgada, The Azores, Portugal, 13/09/2005. https://doi.org/doi:10.1016/j.ics.2005.09.170

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title = "The effect of whole genome amplification on samples originating from more than one donor",

abstract = "In this study, the GenomiPhi(TM) DNA Amplification Kit (Amersham Biosciences) was used to investigate the potential of whole genome amplification (WGA) when considering samples originating from more than one donor. DNA was extracted from blood samples, quantified and normalised before being mixed in ratios of 1:1, 1:3, 1:7 and 1:15. Proportions were checked using standard STR analysis before being subjected to WGA. Once amplified using GenomiPhi(TM), the ratios were again checked. Relative proportions were found to be maintained in the 1:1 and 1:3 ratios following WGA; the observed peak ratios were found to match the expected peak ratios regardless of the starting concentration of DNA. With samples mixed in the ratio of 1:7 and 1:15, and when the concentration of starting material was at the manufacturer's lower limit, too few minor component peaks were found to allow for statistical analysis. With an initial template exceeding 1 ng/[mu]L there was an increase in problems associated with profile interpretation but the results obtained indicated that mixture proportions could be quantifiably maintained",

author = "C.R. Thacker and M.K. Balogh and Claus B{\o}rsting and E. Ramos and Sanchez, {Diz P.} and A. Carracedo and N. Morling and Schneider, {Peter M.} and Denise Syndercombe-Court and {SNPforID Consortium}",

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AU - Thacker, C.R.

AU - Balogh, M.K.

AU - Børsting, Claus

AU - Ramos, E.

AU - Sanchez, Diz P.

AU - Carracedo, A.

AU - Morling, N.

AU - Schneider, Peter M.

AU - Syndercombe-Court, Denise

AU - SNPforID Consortium

PY - 2006

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N2 - In this study, the GenomiPhi(TM) DNA Amplification Kit (Amersham Biosciences) was used to investigate the potential of whole genome amplification (WGA) when considering samples originating from more than one donor. DNA was extracted from blood samples, quantified and normalised before being mixed in ratios of 1:1, 1:3, 1:7 and 1:15. Proportions were checked using standard STR analysis before being subjected to WGA. Once amplified using GenomiPhi(TM), the ratios were again checked. Relative proportions were found to be maintained in the 1:1 and 1:3 ratios following WGA; the observed peak ratios were found to match the expected peak ratios regardless of the starting concentration of DNA. With samples mixed in the ratio of 1:7 and 1:15, and when the concentration of starting material was at the manufacturer's lower limit, too few minor component peaks were found to allow for statistical analysis. With an initial template exceeding 1 ng/[mu]L there was an increase in problems associated with profile interpretation but the results obtained indicated that mixture proportions could be quantifiably maintained

AB - In this study, the GenomiPhi(TM) DNA Amplification Kit (Amersham Biosciences) was used to investigate the potential of whole genome amplification (WGA) when considering samples originating from more than one donor. DNA was extracted from blood samples, quantified and normalised before being mixed in ratios of 1:1, 1:3, 1:7 and 1:15. Proportions were checked using standard STR analysis before being subjected to WGA. Once amplified using GenomiPhi(TM), the ratios were again checked. Relative proportions were found to be maintained in the 1:1 and 1:3 ratios following WGA; the observed peak ratios were found to match the expected peak ratios regardless of the starting concentration of DNA. With samples mixed in the ratio of 1:7 and 1:15, and when the concentration of starting material was at the manufacturer's lower limit, too few minor component peaks were found to allow for statistical analysis. With an initial template exceeding 1 ng/[mu]L there was an increase in problems associated with profile interpretation but the results obtained indicated that mixture proportions could be quantifiably maintained

U2 - doi:10.1016/j.ics.2005.09.170

DO - doi:10.1016/j.ics.2005.09.170

M3 - Article in proceedings

VL - 1288

T3 - ICS - International Congress Series

SP - 722

EP - 724

BT - Progress in Forensic Genetics 11

PB - Elsevier

T2 - Progress in Forensic Genetics 11 - Proceedings of the 21st International ISFG Congress

Y2 - 13 September 2005 through 16 September 2005

ER -

The effect of whole genome amplification on samples originating from more than one donor

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