The Drosophila gene CG9918 codes for a pyrokinin-1 receptor.

Giuseppe Cazzamali; Malene Torp; Frank Hauser; Michael Williamson; Cornelis J P Grimmelikhuijzen

doi:10.1016/j.bbrc.2005.07.038

The Drosophila gene CG9918 codes for a pyrokinin-1 receptor.

Giuseppe Cazzamali, Malene Torp, Frank Hauser, Michael Williamson, Cornelis J P Grimmelikhuijzen

Cell and Neurobiology

92 Citations (Scopus)

Abstract

The database from the Drosophila Genome Project contains a gene, CG9918, annotated to code for a G protein-coupled receptor. We cloned the cDNA of this gene and functionally expressed it in Chinese hamster ovary cells. We tested a library of about 25 Drosophila and other insect neuropeptides, and seven insect biogenic amines on the expressed receptor and found that it was activated by low concentrations of the Drosophila neuropeptide, pyrokinin-1 (TGPSASSGLWFGPRLamide; EC50, 5 x 10(-8) M). The receptor was also activated by other Drosophila neuropeptides, terminating with the sequence PRLamide (Hug-gamma, ecdysis-triggering-hormone-1, pyrokinin-2), but in these cases about six to eight times higher concentrations were needed. The receptor was not activated by Drosophila neuropeptides, containing a C-terminal PRIamide sequence (such as ecdysis-triggering-hormone-2), or PRVamide (such as capa-1 and -2), or other neuropeptides and biogenic amines not related to the pyrokinins. This paper is the first conclusive report that CG9918 is a Drosophila pyrokinin-1 receptor gene.

Original language	English
Journal	Biochemical and Biophysical Research Communications
Volume	335
Issue number	1
Pages (from-to)	14-9
Number of pages	5
ISSN	0006-291X
DOIs	https://doi.org/10.1016/j.bbrc.2005.07.038
Publication status	Published - 2005

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@article{d1a603b0ec2711dcbee902004c4f4f50,

title = "The Drosophila gene CG9918 codes for a pyrokinin-1 receptor.",

abstract = "The database from the Drosophila Genome Project contains a gene, CG9918, annotated to code for a G protein-coupled receptor. We cloned the cDNA of this gene and functionally expressed it in Chinese hamster ovary cells. We tested a library of about 25 Drosophila and other insect neuropeptides, and seven insect biogenic amines on the expressed receptor and found that it was activated by low concentrations of the Drosophila neuropeptide, pyrokinin-1 (TGPSASSGLWFGPRLamide; EC50, 5 x 10(-8) M). The receptor was also activated by other Drosophila neuropeptides, terminating with the sequence PRLamide (Hug-gamma, ecdysis-triggering-hormone-1, pyrokinin-2), but in these cases about six to eight times higher concentrations were needed. The receptor was not activated by Drosophila neuropeptides, containing a C-terminal PRIamide sequence (such as ecdysis-triggering-hormone-2), or PRVamide (such as capa-1 and -2), or other neuropeptides and biogenic amines not related to the pyrokinins. This paper is the first conclusive report that CG9918 is a Drosophila pyrokinin-1 receptor gene.",

author = "Giuseppe Cazzamali and Malene Torp and Frank Hauser and Michael Williamson and Grimmelikhuijzen, {Cornelis J P}",

note = "Keywords: Amino Acid Sequence; Animals; Base Sequence; CHO Cells; Cricetinae; DNA, Complementary; Drosophila; Drosophila Proteins; Exons; Introns; Molecular Sequence Data; Neuropeptides; Phylogeny; Receptors, G-Protein-Coupled; Sequence Alignment; Transcription, Genetic",

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doi = "10.1016/j.bbrc.2005.07.038",

language = "English",

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AU - Cazzamali, Giuseppe

AU - Torp, Malene

AU - Hauser, Frank

AU - Williamson, Michael

AU - Grimmelikhuijzen, Cornelis J P

N1 - Keywords: Amino Acid Sequence; Animals; Base Sequence; CHO Cells; Cricetinae; DNA, Complementary; Drosophila; Drosophila Proteins; Exons; Introns; Molecular Sequence Data; Neuropeptides; Phylogeny; Receptors, G-Protein-Coupled; Sequence Alignment; Transcription, Genetic

PY - 2005

Y1 - 2005

N2 - The database from the Drosophila Genome Project contains a gene, CG9918, annotated to code for a G protein-coupled receptor. We cloned the cDNA of this gene and functionally expressed it in Chinese hamster ovary cells. We tested a library of about 25 Drosophila and other insect neuropeptides, and seven insect biogenic amines on the expressed receptor and found that it was activated by low concentrations of the Drosophila neuropeptide, pyrokinin-1 (TGPSASSGLWFGPRLamide; EC50, 5 x 10(-8) M). The receptor was also activated by other Drosophila neuropeptides, terminating with the sequence PRLamide (Hug-gamma, ecdysis-triggering-hormone-1, pyrokinin-2), but in these cases about six to eight times higher concentrations were needed. The receptor was not activated by Drosophila neuropeptides, containing a C-terminal PRIamide sequence (such as ecdysis-triggering-hormone-2), or PRVamide (such as capa-1 and -2), or other neuropeptides and biogenic amines not related to the pyrokinins. This paper is the first conclusive report that CG9918 is a Drosophila pyrokinin-1 receptor gene.

AB - The database from the Drosophila Genome Project contains a gene, CG9918, annotated to code for a G protein-coupled receptor. We cloned the cDNA of this gene and functionally expressed it in Chinese hamster ovary cells. We tested a library of about 25 Drosophila and other insect neuropeptides, and seven insect biogenic amines on the expressed receptor and found that it was activated by low concentrations of the Drosophila neuropeptide, pyrokinin-1 (TGPSASSGLWFGPRLamide; EC50, 5 x 10(-8) M). The receptor was also activated by other Drosophila neuropeptides, terminating with the sequence PRLamide (Hug-gamma, ecdysis-triggering-hormone-1, pyrokinin-2), but in these cases about six to eight times higher concentrations were needed. The receptor was not activated by Drosophila neuropeptides, containing a C-terminal PRIamide sequence (such as ecdysis-triggering-hormone-2), or PRVamide (such as capa-1 and -2), or other neuropeptides and biogenic amines not related to the pyrokinins. This paper is the first conclusive report that CG9918 is a Drosophila pyrokinin-1 receptor gene.

U2 - 10.1016/j.bbrc.2005.07.038

DO - 10.1016/j.bbrc.2005.07.038

M3 - Journal article

C2 - 16054112

SN - 0006-291X

VL - 335

SP - 14

EP - 19

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

The Drosophila gene CG9918 codes for a pyrokinin-1 receptor.

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