The developing hypopharyngeal microbiota in early life

Martin Steen Mortensen; Asker Daniel Brejnrod; Michael Roggenbuck; Waleed Abu Al-Soud; Christina Balle; Karen Angeliki Krogfelt; Jakob Stokholm; Jonathan Thorsen; Johannes Eichler Waage; Morten Arendt Rasmussen; Hans Bisgaard; Søren Johannes Sørensen

doi:10.1186/s40168-016-0215-9

The developing hypopharyngeal microbiota in early life

Martin Steen Mortensen, Asker Daniel Brejnrod, Michael Roggenbuck, Waleed Abu Al-Soud, Christina Balle, Karen Angeliki Krogfelt, Jakob Stokholm, Jonathan Thorsen, Johannes Eichler Waage, Morten Arendt Rasmussen, Hans Bisgaard, Søren Johannes Sørensen

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Abstract

BACKGROUND: The airways of healthy humans harbor a distinct microbial community. Perturbations in the microbial community have been associated with disease, yet little is known about the formation and development of a healthy airway microbiota in early life. Our goal was to understand the establishment of the airway microbiota within the first 3 months of life. We investigated the hypopharyngeal microbiota in the unselected COPSAC2010 cohort of 700 infants, using 16S rRNA gene sequencing of hypopharyngeal aspirates from 1 week, 1 month, and 3 months of age.

RESULTS: Our analysis shows that majority of the hypopharyngeal microbiota of healthy infants belong to each individual's core microbiota and we demonstrate five distinct community pneumotypes. Four of these pneumotypes are dominated by the genera Staphylococcus, Streptococcus, Moraxella, and Corynebacterium, respectively. Furthermore, we show temporal pneumotype changes suggesting a rapid development towards maturation of the hypopharyngeal microbiota and a significant effect from older siblings. Despite an overall common trajectory towards maturation, individual infants' microbiota are more similar to their own, than to others, over time.

CONCLUSIONS: Our findings demonstrate a consolidation of the population of indigenous bacteria in healthy airways and indicate distinct trajectories in the early development of the hypopharyngeal microbiota.

Original language	English
Article number	70
Journal	Microbiome
Volume	4
Number of pages	12
ISSN	2049-2618
DOIs	https://doi.org/10.1186/s40168-016-0215-9
Publication status	Published - 30 Dec 2016

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title = "The developing hypopharyngeal microbiota in early life",

abstract = "BACKGROUND: The airways of healthy humans harbor a distinct microbial community. Perturbations in the microbial community have been associated with disease, yet little is known about the formation and development of a healthy airway microbiota in early life. Our goal was to understand the establishment of the airway microbiota within the first 3 months of life. We investigated the hypopharyngeal microbiota in the unselected COPSAC2010 cohort of 700 infants, using 16S rRNA gene sequencing of hypopharyngeal aspirates from 1 week, 1 month, and 3 months of age.RESULTS: Our analysis shows that majority of the hypopharyngeal microbiota of healthy infants belong to each individual's core microbiota and we demonstrate five distinct community pneumotypes. Four of these pneumotypes are dominated by the genera Staphylococcus, Streptococcus, Moraxella, and Corynebacterium, respectively. Furthermore, we show temporal pneumotype changes suggesting a rapid development towards maturation of the hypopharyngeal microbiota and a significant effect from older siblings. Despite an overall common trajectory towards maturation, individual infants' microbiota are more similar to their own, than to others, over time.CONCLUSIONS: Our findings demonstrate a consolidation of the population of indigenous bacteria in healthy airways and indicate distinct trajectories in the early development of the hypopharyngeal microbiota.",

author = "Mortensen, {Martin Steen} and Brejnrod, {Asker Daniel} and Michael Roggenbuck and {Abu Al-Soud}, Waleed and Christina Balle and Krogfelt, {Karen Angeliki} and Jakob Stokholm and Jonathan Thorsen and Waage, {Johannes Eichler} and Rasmussen, {Morten Arendt} and Hans Bisgaard and S{\o}rensen, {S{\o}ren Johannes}",

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doi = "10.1186/s40168-016-0215-9",

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journal = "Microbiome",

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T1 - The developing hypopharyngeal microbiota in early life

AU - Mortensen, Martin Steen

AU - Brejnrod, Asker Daniel

AU - Roggenbuck, Michael

AU - Abu Al-Soud, Waleed

AU - Balle, Christina

AU - Krogfelt, Karen Angeliki

AU - Stokholm, Jakob

AU - Thorsen, Jonathan

AU - Waage, Johannes Eichler

AU - Rasmussen, Morten Arendt

AU - Bisgaard, Hans

AU - Sørensen, Søren Johannes

PY - 2016/12/30

Y1 - 2016/12/30

N2 - BACKGROUND: The airways of healthy humans harbor a distinct microbial community. Perturbations in the microbial community have been associated with disease, yet little is known about the formation and development of a healthy airway microbiota in early life. Our goal was to understand the establishment of the airway microbiota within the first 3 months of life. We investigated the hypopharyngeal microbiota in the unselected COPSAC2010 cohort of 700 infants, using 16S rRNA gene sequencing of hypopharyngeal aspirates from 1 week, 1 month, and 3 months of age.RESULTS: Our analysis shows that majority of the hypopharyngeal microbiota of healthy infants belong to each individual's core microbiota and we demonstrate five distinct community pneumotypes. Four of these pneumotypes are dominated by the genera Staphylococcus, Streptococcus, Moraxella, and Corynebacterium, respectively. Furthermore, we show temporal pneumotype changes suggesting a rapid development towards maturation of the hypopharyngeal microbiota and a significant effect from older siblings. Despite an overall common trajectory towards maturation, individual infants' microbiota are more similar to their own, than to others, over time.CONCLUSIONS: Our findings demonstrate a consolidation of the population of indigenous bacteria in healthy airways and indicate distinct trajectories in the early development of the hypopharyngeal microbiota.

AB - BACKGROUND: The airways of healthy humans harbor a distinct microbial community. Perturbations in the microbial community have been associated with disease, yet little is known about the formation and development of a healthy airway microbiota in early life. Our goal was to understand the establishment of the airway microbiota within the first 3 months of life. We investigated the hypopharyngeal microbiota in the unselected COPSAC2010 cohort of 700 infants, using 16S rRNA gene sequencing of hypopharyngeal aspirates from 1 week, 1 month, and 3 months of age.RESULTS: Our analysis shows that majority of the hypopharyngeal microbiota of healthy infants belong to each individual's core microbiota and we demonstrate five distinct community pneumotypes. Four of these pneumotypes are dominated by the genera Staphylococcus, Streptococcus, Moraxella, and Corynebacterium, respectively. Furthermore, we show temporal pneumotype changes suggesting a rapid development towards maturation of the hypopharyngeal microbiota and a significant effect from older siblings. Despite an overall common trajectory towards maturation, individual infants' microbiota are more similar to their own, than to others, over time.CONCLUSIONS: Our findings demonstrate a consolidation of the population of indigenous bacteria in healthy airways and indicate distinct trajectories in the early development of the hypopharyngeal microbiota.

U2 - 10.1186/s40168-016-0215-9

DO - 10.1186/s40168-016-0215-9

M3 - Journal article

C2 - 28038686

SN - 2049-2618

VL - 4

JO - Microbiome

JF - Microbiome

M1 - 70

ER -

The developing hypopharyngeal microbiota in early life

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