The 5-HT1A serotonin receptor is located on calbindin- and parvalbumin-containing neurons in the rat brain

TY - JOUR

T1 - The 5-HT1A serotonin receptor is located on calbindin- and parvalbumin-containing neurons in the rat brain

AU - Aznar, Susana

AU - Qian, Zhaoxia

AU - Shah, Reshma

AU - Rahbek, Birgitte

AU - Knudsen, Gitte M

PY - 2003/1/3

Y1 - 2003/1/3

N2 - The 5-HT(1A) receptor is a well-characterized serotonin receptor playing a role in many central nervous functions and known to be involved in depression and other mental disorders. In situ hybridization, immunocytochemical, and binding studies have shown that the 5-HT(1A) receptor is widely distributed in the rat brain, with a particularly high density in the limbic system. The receptor's localization in the different neuronal subtypes, which may be of importance for understanding its role in neuronal circuitries, is, however, unknown. In this study we show by immunocytochemical double-labeling techniques, that the 5-HT(1A) receptor is present on both pyramidal and principal cells, and calbindin- and parvalbumin-containing neurons, which generally define two different subtypes of interneurons. Moreover, semiquantitative analysis showed that the receptor's distribution in the different neuronal types varies between brain areas. In cortex, hippocampus, hypothalamus, and amygdala the receptor was located on both principal cells and calbindin- and parvalbumin-containing neurons. In septum and thalamus, the receptor was mostly present on calbindin- and parvalbumin-containing cells. Especially in the medial septum and thalamic reticular nucleus, the receptor highly colocalized with parvalbumin-positive neurons. These results suggest a diverse function of the 5-HT(1A) receptor in modulating neuronal circuitry in different brain areas, that may depend on the type of neuron the receptor is predominantly located on.

AB - The 5-HT(1A) receptor is a well-characterized serotonin receptor playing a role in many central nervous functions and known to be involved in depression and other mental disorders. In situ hybridization, immunocytochemical, and binding studies have shown that the 5-HT(1A) receptor is widely distributed in the rat brain, with a particularly high density in the limbic system. The receptor's localization in the different neuronal subtypes, which may be of importance for understanding its role in neuronal circuitries, is, however, unknown. In this study we show by immunocytochemical double-labeling techniques, that the 5-HT(1A) receptor is present on both pyramidal and principal cells, and calbindin- and parvalbumin-containing neurons, which generally define two different subtypes of interneurons. Moreover, semiquantitative analysis showed that the receptor's distribution in the different neuronal types varies between brain areas. In cortex, hippocampus, hypothalamus, and amygdala the receptor was located on both principal cells and calbindin- and parvalbumin-containing neurons. In septum and thalamus, the receptor was mostly present on calbindin- and parvalbumin-containing cells. Especially in the medial septum and thalamic reticular nucleus, the receptor highly colocalized with parvalbumin-positive neurons. These results suggest a diverse function of the 5-HT(1A) receptor in modulating neuronal circuitry in different brain areas, that may depend on the type of neuron the receptor is predominantly located on.

KW - Animals

KW - Brain/anatomy & histology

KW - Brain Chemistry

KW - Calbindins

KW - Immunohistochemistry

KW - Male

KW - Neurons/cytology

KW - Parvalbumins/metabolism

KW - Rats

KW - Rats, Sprague-Dawley

KW - Receptors, Serotonin/metabolism

KW - Receptors, Serotonin, 5-HT1

KW - S100 Calcium Binding Protein G/metabolism

M3 - Journal article

C2 - 12480158

SN - 0006-8993

VL - 959

SP - 58

EP - 67

JO - Brain Research

JF - Brain Research

IS - 1

ER -