Testing of the Illumina® ForenSeq™ kit

C. Hussing; C. Børsting; H. S. Mogensen; N. Morling

doi:10.1016/j.fsigss.2015.09.178

Testing of the Illumina^® ForenSeq™ kit

C. Hussing^*, C. Børsting, H. S. Mogensen, N. Morling

^*Corresponding author for this work

5 Citations (Scopus)

Abstract

The Illumina® ForenSeq™ kit amplifies in one multiplex PCR reaction 59 STRs and 172 SNPs relevant to forensic genetic case work. Typed markers include markers for identification, ancestry identification (AIMs) and pigmentary traits. The products were sequenced on the MiSeq FGx Forensic Genomics System and analysed with the ForenSeq™ Universal Analysis Software. Using this approach, we sequenced 10 Danish, 10 Italian, and 10 individuals from other populations, mostly non-europeans. The eye and hair colours as well as the bio-geographical ancestry of the individuals were known.Blue and brown eye colours were predicted accurately by the ForenSeq™ assay, whereas intermediate eye colours were not. The success of the hair colour prediction tool seemed population-dependent. The predictions matched the hair colours reported by the individuals themselves in twice as many Danes as Italians. The geographic ancestries of 22 out of 23 individuals of European or East Asian ancestry were correctly predicted. However, the ForenSeq™ Universal Analysis Software was unable to differentiate between individuals from Middle East/South Asia, South America and North Africa.We found no difference in STR typing results obtained with the ForenSeq™ kit and capillary electrophoresis.

Original language	English
Journal	Forensic Science International: Genetics Supplement Series
Volume	5
Pages (from-to)	e449-e450
Number of pages	2
ISSN	1875-1768
DOIs	https://doi.org/10.1016/j.fsigss.2015.09.178
Publication status	Published - 1 Dec 2015
Event	26th Congress of the International Society of Forensic Genetics - Krakow, Poland Duration: 31 Aug 2015 → 5 Sept 2015

Conference

Conference	26th Congress of the International Society of Forensic Genetics
Country/Territory	Poland
City	Krakow
Period	31/08/2015 → 05/09/2015

Keywords

AIMs
ForenSeq™
Forensic genomics
Hirisplex
Illumina
MiSeq FGx

Access to Document

10.1016/j.fsigss.2015.09.178

http://www.fsigeneticssup.com/article/S1875176815301566/pdf

Cite this

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title = "Testing of the Illumina{\textregistered} ForenSeq{\texttrademark} kit",

abstract = "The Illumina{\textregistered} ForenSeq{\texttrademark} kit amplifies in one multiplex PCR reaction 59 STRs and 172 SNPs relevant to forensic genetic case work. Typed markers include markers for identification, ancestry identification (AIMs) and pigmentary traits. The products were sequenced on the MiSeq FGx Forensic Genomics System and analysed with the ForenSeq{\texttrademark} Universal Analysis Software. Using this approach, we sequenced 10 Danish, 10 Italian, and 10 individuals from other populations, mostly non-europeans. The eye and hair colours as well as the bio-geographical ancestry of the individuals were known.Blue and brown eye colours were predicted accurately by the ForenSeq{\texttrademark} assay, whereas intermediate eye colours were not. The success of the hair colour prediction tool seemed population-dependent. The predictions matched the hair colours reported by the individuals themselves in twice as many Danes as Italians. The geographic ancestries of 22 out of 23 individuals of European or East Asian ancestry were correctly predicted. However, the ForenSeq{\texttrademark} Universal Analysis Software was unable to differentiate between individuals from Middle East/South Asia, South America and North Africa.We found no difference in STR typing results obtained with the ForenSeq{\texttrademark} kit and capillary electrophoresis.",

keywords = "AIMs, ForenSeq{\texttrademark}, Forensic genomics, Hirisplex, Illumina, MiSeq FGx",

author = "C. Hussing and C. B{\o}rsting and Mogensen, {H. S.} and N. Morling",

year = "2015",

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doi = "10.1016/j.fsigss.2015.09.178",

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pages = "e449--e450",

journal = "Forensic Science International: Genetics. Supplement Series",

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AU - Hussing, C.

AU - Børsting, C.

AU - Mogensen, H. S.

AU - Morling, N.

PY - 2015/12/1

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N2 - The Illumina® ForenSeq™ kit amplifies in one multiplex PCR reaction 59 STRs and 172 SNPs relevant to forensic genetic case work. Typed markers include markers for identification, ancestry identification (AIMs) and pigmentary traits. The products were sequenced on the MiSeq FGx Forensic Genomics System and analysed with the ForenSeq™ Universal Analysis Software. Using this approach, we sequenced 10 Danish, 10 Italian, and 10 individuals from other populations, mostly non-europeans. The eye and hair colours as well as the bio-geographical ancestry of the individuals were known.Blue and brown eye colours were predicted accurately by the ForenSeq™ assay, whereas intermediate eye colours were not. The success of the hair colour prediction tool seemed population-dependent. The predictions matched the hair colours reported by the individuals themselves in twice as many Danes as Italians. The geographic ancestries of 22 out of 23 individuals of European or East Asian ancestry were correctly predicted. However, the ForenSeq™ Universal Analysis Software was unable to differentiate between individuals from Middle East/South Asia, South America and North Africa.We found no difference in STR typing results obtained with the ForenSeq™ kit and capillary electrophoresis.

AB - The Illumina® ForenSeq™ kit amplifies in one multiplex PCR reaction 59 STRs and 172 SNPs relevant to forensic genetic case work. Typed markers include markers for identification, ancestry identification (AIMs) and pigmentary traits. The products were sequenced on the MiSeq FGx Forensic Genomics System and analysed with the ForenSeq™ Universal Analysis Software. Using this approach, we sequenced 10 Danish, 10 Italian, and 10 individuals from other populations, mostly non-europeans. The eye and hair colours as well as the bio-geographical ancestry of the individuals were known.Blue and brown eye colours were predicted accurately by the ForenSeq™ assay, whereas intermediate eye colours were not. The success of the hair colour prediction tool seemed population-dependent. The predictions matched the hair colours reported by the individuals themselves in twice as many Danes as Italians. The geographic ancestries of 22 out of 23 individuals of European or East Asian ancestry were correctly predicted. However, the ForenSeq™ Universal Analysis Software was unable to differentiate between individuals from Middle East/South Asia, South America and North Africa.We found no difference in STR typing results obtained with the ForenSeq™ kit and capillary electrophoresis.

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KW - ForenSeq™

KW - Forensic genomics

KW - Hirisplex

KW - Illumina

KW - MiSeq FGx

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DO - 10.1016/j.fsigss.2015.09.178

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VL - 5

SP - e449-e450

JO - Forensic Science International: Genetics. Supplement Series

JF - Forensic Science International: Genetics. Supplement Series

T2 - 26th Congress of the International Society of Forensic Genetics

Y2 - 31 August 2015 through 5 September 2015

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