Targeted integration of genes in Xenopus tropicalis

Zhaoying Shi; Dandan Tian; Huhu Xin; Jingru Lian; Xiaogang Guo; Yonglong Chen

doi:10.1002/dvg.23006

Targeted integration of genes in Xenopus tropicalis

Zhaoying Shi, Dandan Tian, Huhu Xin, Jingru Lian, Xiaogang Guo, Yonglong Chen

Laboratory

5 Citations (Scopus)

Abstract

With the successful establishment of both targeted gene disruption and integration methods in the true diploid frog Xenopus tropicalis, this excellent vertebrate genetic model now is making a unique contribution to modelling human diseases. Here, we summarize our efforts on establishing homologous recombination-mediated targeted integration in Xenopus tropicalis, the usefulness, and limitation of targeted integration via the homology-independent strategy, and future directions on how to further improve targeted gene integration in Xenopus tropicalis.

Original language	English
Article number	e23006
Journal	Genesis: The Journal of Genetics and Development (Online)
Volume	55
Issue number	1-2
Number of pages	6
ISSN	1526-968X
DOIs	https://doi.org/10.1002/dvg.23006
Publication status	Published - 1 Jan 2017

Keywords

Animals
Disease Models, Animal
Gene Expression Regulation, Developmental
Gene Targeting
Humans
Xenopus
Xenopus Proteins
Journal Article
Review

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/dvg.23006

Cite this

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title = "Targeted integration of genes in Xenopus tropicalis",

abstract = "With the successful establishment of both targeted gene disruption and integration methods in the true diploid frog Xenopus tropicalis, this excellent vertebrate genetic model now is making a unique contribution to modelling human diseases. Here, we summarize our efforts on establishing homologous recombination-mediated targeted integration in Xenopus tropicalis, the usefulness, and limitation of targeted integration via the homology-independent strategy, and future directions on how to further improve targeted gene integration in Xenopus tropicalis.",

keywords = "Animals, Disease Models, Animal, Gene Expression Regulation, Developmental, Gene Targeting, Humans, Xenopus, Xenopus Proteins, Journal Article, Review",

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T1 - Targeted integration of genes in Xenopus tropicalis

AU - Shi, Zhaoying

AU - Tian, Dandan

AU - Xin, Huhu

AU - Lian, Jingru

AU - Guo, Xiaogang

AU - Chen, Yonglong

PY - 2017/1/1

Y1 - 2017/1/1

N2 - With the successful establishment of both targeted gene disruption and integration methods in the true diploid frog Xenopus tropicalis, this excellent vertebrate genetic model now is making a unique contribution to modelling human diseases. Here, we summarize our efforts on establishing homologous recombination-mediated targeted integration in Xenopus tropicalis, the usefulness, and limitation of targeted integration via the homology-independent strategy, and future directions on how to further improve targeted gene integration in Xenopus tropicalis.

AB - With the successful establishment of both targeted gene disruption and integration methods in the true diploid frog Xenopus tropicalis, this excellent vertebrate genetic model now is making a unique contribution to modelling human diseases. Here, we summarize our efforts on establishing homologous recombination-mediated targeted integration in Xenopus tropicalis, the usefulness, and limitation of targeted integration via the homology-independent strategy, and future directions on how to further improve targeted gene integration in Xenopus tropicalis.

KW - Animals

KW - Disease Models, Animal

KW - Gene Expression Regulation, Developmental

KW - Gene Targeting

KW - Humans

KW - Xenopus

KW - Xenopus Proteins

KW - Journal Article

KW - Review

U2 - 10.1002/dvg.23006

DO - 10.1002/dvg.23006

M3 - Review

C2 - 28095621

SN - 1526-968X

VL - 55

JO - Genesis: The Journal of Genetics and Development (Online)

JF - Genesis: The Journal of Genetics and Development (Online)

IS - 1-2

M1 - e23006

ER -

Targeted integration of genes in Xenopus tropicalis

Abstract

Keywords

UN SDGs

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