Targeted integration of genes in Xenopus tropicalis

TY - JOUR

T1 - Targeted integration of genes in Xenopus tropicalis

AU - Shi, Zhaoying

AU - Tian, Dandan

AU - Xin, Huhu

AU - Lian, Jingru

AU - Guo, Xiaogang

AU - Chen, Yonglong

N1 - © 2017 Wiley Periodicals, Inc.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - With the successful establishment of both targeted gene disruption and integration methods in the true diploid frog Xenopus tropicalis, this excellent vertebrate genetic model now is making a unique contribution to modelling human diseases. Here, we summarize our efforts on establishing homologous recombination-mediated targeted integration in Xenopus tropicalis, the usefulness, and limitation of targeted integration via the homology-independent strategy, and future directions on how to further improve targeted gene integration in Xenopus tropicalis.

AB - With the successful establishment of both targeted gene disruption and integration methods in the true diploid frog Xenopus tropicalis, this excellent vertebrate genetic model now is making a unique contribution to modelling human diseases. Here, we summarize our efforts on establishing homologous recombination-mediated targeted integration in Xenopus tropicalis, the usefulness, and limitation of targeted integration via the homology-independent strategy, and future directions on how to further improve targeted gene integration in Xenopus tropicalis.

KW - Animals

KW - Disease Models, Animal

KW - Gene Expression Regulation, Developmental

KW - Gene Targeting

KW - Humans

KW - Xenopus

KW - Xenopus Proteins

KW - Journal Article

KW - Review

U2 - 10.1002/dvg.23006

DO - 10.1002/dvg.23006

M3 - Review

C2 - 28095621

SN - 1526-968X

VL - 55

JO - Genesis: The Journal of Genetics and Development (Online)

JF - Genesis: The Journal of Genetics and Development (Online)

IS - 1-2

M1 - e23006

ER -