Abstract
The permeability-enhancing effects of the two surfactants, 1-palmitoyl-2-lyso-sn-gycero-3-phosphocholine (lysoPPC) and palmitic acid (PA), on lipid membranes that at physiological temperatures are in the gel, fluid, and liquid-ordered phases were determined using the concentration-dependent self-quenching properties of the hydrophilic marker, calcein. Adding lysoPPC to lipid membranes in the gel-phase induced a time-dependent calcein release curve that can be described by the sum of two exponentials, whereas PA induces a considerably more complex release curve. However, when lysoPPC and PA were added simultaneously in equimolar concentrations, a dramatic synergistic permeability-enhancing effect was observed. In contrast, when both lysoPPC and PA are added to liposomal membranes that are in the fluid or liquid-ordered phases, no effect on the transmembrane permeation of calcein was observed.
| Original language | English |
|---|---|
| Journal | Biochimica et Biophysica Acta - Biomembranes |
| Volume | 1564 |
| Issue number | 1 |
| Pages (from-to) | 256-262 |
| Number of pages | 7 |
| ISSN | 0005-2736 |
| DOIs | |
| Publication status | Published - 19 Aug 2002 |
Keywords
- Drug-delivery
- Enhancer
- Fatty acid
- Liposome
- Lysophospholipid
- Membrane
- Permeability
- Surfactant