Synergistic permeability enhancing effect of lysophospholipids and fatty acids on lipid membranes

TY - JOUR

T1 - Synergistic permeability enhancing effect of lysophospholipids and fatty acids on lipid membranes

AU - Davidsen, Jesper

AU - Mouritsen, Ole G.

AU - Jorgensen, Kent

PY - 2002/8/19

Y1 - 2002/8/19

N2 - The permeability-enhancing effects of the two surfactants, 1-palmitoyl-2-lyso-sn-gycero-3-phosphocholine (lysoPPC) and palmitic acid (PA), on lipid membranes that at physiological temperatures are in the gel, fluid, and liquid-ordered phases were determined using the concentration-dependent self-quenching properties of the hydrophilic marker, calcein. Adding lysoPPC to lipid membranes in the gel-phase induced a time-dependent calcein release curve that can be described by the sum of two exponentials, whereas PA induces a considerably more complex release curve. However, when lysoPPC and PA were added simultaneously in equimolar concentrations, a dramatic synergistic permeability-enhancing effect was observed. In contrast, when both lysoPPC and PA are added to liposomal membranes that are in the fluid or liquid-ordered phases, no effect on the transmembrane permeation of calcein was observed.

AB - The permeability-enhancing effects of the two surfactants, 1-palmitoyl-2-lyso-sn-gycero-3-phosphocholine (lysoPPC) and palmitic acid (PA), on lipid membranes that at physiological temperatures are in the gel, fluid, and liquid-ordered phases were determined using the concentration-dependent self-quenching properties of the hydrophilic marker, calcein. Adding lysoPPC to lipid membranes in the gel-phase induced a time-dependent calcein release curve that can be described by the sum of two exponentials, whereas PA induces a considerably more complex release curve. However, when lysoPPC and PA were added simultaneously in equimolar concentrations, a dramatic synergistic permeability-enhancing effect was observed. In contrast, when both lysoPPC and PA are added to liposomal membranes that are in the fluid or liquid-ordered phases, no effect on the transmembrane permeation of calcein was observed.

KW - Drug-delivery

KW - Enhancer

KW - Fatty acid

KW - Liposome

KW - Lysophospholipid

KW - Membrane

KW - Permeability

KW - Surfactant

UR - http://www.scopus.com/inward/record.url?scp=0037136068&partnerID=8YFLogxK

U2 - 10.1016/S0005-2736(02)00461-3

DO - 10.1016/S0005-2736(02)00461-3

M3 - Journal article

C2 - 12101020

AN - SCOPUS:0037136068

SN - 0005-2736

VL - 1564

SP - 256

EP - 262

JO - B B A - Biomembranes

JF - B B A - Biomembranes

IS - 1

ER -