Abstract
A well-characterized S phase, a unicellular lifestyle, and a plethora of mutations in key components of DNA metabolism make fission yeast a particularly attractive system in which to study DNA replication. However, synchronization of passage through a normal S phase has proved challenging. This protocol describes how combining nitrogen starvation with M-factor mating pheromone treatment presents a highly effective method for synchronizing passage through an ostensibly normal S phase.
| Original language | English |
|---|---|
| Journal | Cold Spring Harbor Protocols |
| Volume | 2016 |
| Issue number | 9 |
| Pages (from-to) | 768-772 |
| Number of pages | 5 |
| ISSN | 1940-3402 |
| DOIs | |
| Publication status | Published - 2016 |