Synchronization of S phase in Schizosaccharomyces pombe cells by transient exposure to M-factor pheromone

TY - JOUR

T1 - Synchronization of S phase in Schizosaccharomyces pombe cells by transient exposure to M-factor pheromone

AU - Nielsen, Olaf

PY - 2016

Y1 - 2016

N2 - A well-characterized S phase, a unicellular lifestyle, and a plethora of mutations in key components of DNA metabolism make fission yeast a particularly attractive system in which to study DNA replication. However, synchronization of passage through a normal S phase has proved challenging. This protocol describes how combining nitrogen starvation with M-factor mating pheromone treatment presents a highly effective method for synchronizing passage through an ostensibly normal S phase.

AB - A well-characterized S phase, a unicellular lifestyle, and a plethora of mutations in key components of DNA metabolism make fission yeast a particularly attractive system in which to study DNA replication. However, synchronization of passage through a normal S phase has proved challenging. This protocol describes how combining nitrogen starvation with M-factor mating pheromone treatment presents a highly effective method for synchronizing passage through an ostensibly normal S phase.

U2 - 10.1101/pdb.prot091272

DO - 10.1101/pdb.prot091272

M3 - Journal article

C2 - 27587782

AN - SCOPUS:84985945892

SN - 1940-3402

VL - 2016

SP - 768

EP - 772

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

IS - 9

ER -