Survival of lactic acid and chlorine dioxide treated Campylobacter jejuni under suboptimal conditions of pH, temperature and modified atmosphere

Nada Smigic; Andreja Rajkovic; Dennis Sandris Nielsen; Nils Arneborg; Henrik Siegumfeldt; Frank Devlieghere

doi:10.1016/j.ijfoodmicro.2010.01.026

Survival of lactic acid and chlorine dioxide treated Campylobacter jejuni under suboptimal conditions of pH, temperature and modified atmosphere

Nada Smigic, Andreja Rajkovic, Dennis Sandris Nielsen, Nils Arneborg, Henrik Siegumfeldt, Frank Devlieghere

16 Citations (Scopus)

Abstract

As mild decontamination treatments are gaining more and more interest due to increased consumer demands for fresh foods, it is of great importance to establish the influence of decontamination treatments on the subsequent bacterial behaviour under suboptimal storage conditions. For this purpose Campylobacter jejuni cells treated with lactic acid (LA, 3% lactic acid, pH 4.0, 2min) or chlorine dioxide (ClO₂, 20ppm, 2min) were inoculated in Bolton broth (pH 6.0) and incubated under 80% O₂/20% N₂, 80% CO₂/20% N₂, air or micro-aerophilic (10% CO₂/85% N₂/5% O₂) atmosphere, at 4°C during 7days. Treatment with water served as a control. The most suppressive atmosphere for the survival of C. jejuni was O₂-rich atmosphere, followed by air, micro-aerophilic and CO₂-rich atmosphere. The survival of C. jejuni was dependent on the type of initial decontamination treatment, with water treated cells showing the greatest survival followed by LA and ClO₂ treated cells. Intracellular pH (pH_i) of individual C. jejuni cells was determined using Fluorescence Ratio Imaging Microscopy (FRIM). At all tested conditions, different subpopulation of the cells could be distinguished based on their pH_i values. The pH_i response was independent on the surrounding atmosphere since similar distribution of the subpopulations was observed for all tested atmospheres. However, the pH_i response was dependent on the initial decontamination treatment. The investigation of intracellular parameters gave an insight into pathogen behaviour under stressful conditions at intracellular level. The results obtained in this study highlighted the importance of combining decontamination technologies with subsequent preservation techniques to the control survival and growth of foodborne pathogens.

Original language	English
Journal	International Journal of Food Microbiology
Volume	141
Issue number	Suppl. 1
Pages (from-to)	S140-S146
Number of pages	7
ISSN	0168-1605
DOIs	https://doi.org/10.1016/j.ijfoodmicro.2010.01.026
Publication status	Published - Jul 2010

Keywords

Former LIFE faculty

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10.1016/j.ijfoodmicro.2010.01.026

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Survival of lactic acid and chlorine dioxide treated Campylobacter jejuni under suboptimal conditions of pH, temperature and modified atmosphere. / Smigic, Nada; Rajkovic, Andreja; Nielsen, Dennis Sandris et al.

In: International Journal of Food Microbiology, Vol. 141, No. Suppl. 1, 07.2010, p. S140-S146.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Survival of lactic acid and chlorine dioxide treated Campylobacter jejuni under suboptimal conditions of pH, temperature and modified atmosphere",

abstract = "As mild decontamination treatments are gaining more and more interest due to increased consumer demands for fresh foods, it is of great importance to establish the influence of decontamination treatments on the subsequent bacterial behaviour under suboptimal storage conditions. For this purpose Campylobacter jejuni cells treated with lactic acid (LA, 3% lactic acid, pH 4.0, 2min) or chlorine dioxide (ClO2, 20ppm, 2min) were inoculated in Bolton broth (pH 6.0) and incubated under 80% O2/20% N2, 80% CO2/20% N2, air or micro-aerophilic (10% CO2/85% N2/5% O2) atmosphere, at 4°C during 7days. Treatment with water served as a control. The most suppressive atmosphere for the survival of C. jejuni was O2-rich atmosphere, followed by air, micro-aerophilic and CO2-rich atmosphere. The survival of C. jejuni was dependent on the type of initial decontamination treatment, with water treated cells showing the greatest survival followed by LA and ClO2 treated cells. Intracellular pH (pHi) of individual C. jejuni cells was determined using Fluorescence Ratio Imaging Microscopy (FRIM). At all tested conditions, different subpopulation of the cells could be distinguished based on their pHi values. The pHi response was independent on the surrounding atmosphere since similar distribution of the subpopulations was observed for all tested atmospheres. However, the pHi response was dependent on the initial decontamination treatment. The investigation of intracellular parameters gave an insight into pathogen behaviour under stressful conditions at intracellular level. The results obtained in this study highlighted the importance of combining decontamination technologies with subsequent preservation techniques to the control survival and growth of foodborne pathogens.",

keywords = "Former LIFE faculty, Campylobacter jejuni, Lactic acid, Chlorine dioxide, Modified atmosphere, Intracellular pH",

author = "Nada Smigic and Andreja Rajkovic and Nielsen, {Dennis Sandris} and Nils Arneborg and Henrik Siegumfeldt and Frank Devlieghere",

year = "2010",

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T1 - Survival of lactic acid and chlorine dioxide treated Campylobacter jejuni under suboptimal conditions of pH, temperature and modified atmosphere

AU - Smigic, Nada

AU - Rajkovic, Andreja

AU - Nielsen, Dennis Sandris

AU - Arneborg, Nils

AU - Siegumfeldt, Henrik

AU - Devlieghere, Frank

PY - 2010/7

Y1 - 2010/7

N2 - As mild decontamination treatments are gaining more and more interest due to increased consumer demands for fresh foods, it is of great importance to establish the influence of decontamination treatments on the subsequent bacterial behaviour under suboptimal storage conditions. For this purpose Campylobacter jejuni cells treated with lactic acid (LA, 3% lactic acid, pH 4.0, 2min) or chlorine dioxide (ClO2, 20ppm, 2min) were inoculated in Bolton broth (pH 6.0) and incubated under 80% O2/20% N2, 80% CO2/20% N2, air or micro-aerophilic (10% CO2/85% N2/5% O2) atmosphere, at 4°C during 7days. Treatment with water served as a control. The most suppressive atmosphere for the survival of C. jejuni was O2-rich atmosphere, followed by air, micro-aerophilic and CO2-rich atmosphere. The survival of C. jejuni was dependent on the type of initial decontamination treatment, with water treated cells showing the greatest survival followed by LA and ClO2 treated cells. Intracellular pH (pHi) of individual C. jejuni cells was determined using Fluorescence Ratio Imaging Microscopy (FRIM). At all tested conditions, different subpopulation of the cells could be distinguished based on their pHi values. The pHi response was independent on the surrounding atmosphere since similar distribution of the subpopulations was observed for all tested atmospheres. However, the pHi response was dependent on the initial decontamination treatment. The investigation of intracellular parameters gave an insight into pathogen behaviour under stressful conditions at intracellular level. The results obtained in this study highlighted the importance of combining decontamination technologies with subsequent preservation techniques to the control survival and growth of foodborne pathogens.

AB - As mild decontamination treatments are gaining more and more interest due to increased consumer demands for fresh foods, it is of great importance to establish the influence of decontamination treatments on the subsequent bacterial behaviour under suboptimal storage conditions. For this purpose Campylobacter jejuni cells treated with lactic acid (LA, 3% lactic acid, pH 4.0, 2min) or chlorine dioxide (ClO2, 20ppm, 2min) were inoculated in Bolton broth (pH 6.0) and incubated under 80% O2/20% N2, 80% CO2/20% N2, air or micro-aerophilic (10% CO2/85% N2/5% O2) atmosphere, at 4°C during 7days. Treatment with water served as a control. The most suppressive atmosphere for the survival of C. jejuni was O2-rich atmosphere, followed by air, micro-aerophilic and CO2-rich atmosphere. The survival of C. jejuni was dependent on the type of initial decontamination treatment, with water treated cells showing the greatest survival followed by LA and ClO2 treated cells. Intracellular pH (pHi) of individual C. jejuni cells was determined using Fluorescence Ratio Imaging Microscopy (FRIM). At all tested conditions, different subpopulation of the cells could be distinguished based on their pHi values. The pHi response was independent on the surrounding atmosphere since similar distribution of the subpopulations was observed for all tested atmospheres. However, the pHi response was dependent on the initial decontamination treatment. The investigation of intracellular parameters gave an insight into pathogen behaviour under stressful conditions at intracellular level. The results obtained in this study highlighted the importance of combining decontamination technologies with subsequent preservation techniques to the control survival and growth of foodborne pathogens.

KW - Former LIFE faculty

KW - Campylobacter jejuni

KW - Lactic acid

KW - Chlorine dioxide

KW - Modified atmosphere

KW - Intracellular pH

U2 - 10.1016/j.ijfoodmicro.2010.01.026

DO - 10.1016/j.ijfoodmicro.2010.01.026

M3 - Journal article

SN - 0168-1605

VL - 141

SP - S140-S146

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

IS - Suppl. 1

ER -

Survival of lactic acid and chlorine dioxide treated Campylobacter jejuni under suboptimal conditions of pH, temperature and modified atmosphere

Abstract

Keywords

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