TY - JOUR
T1 - Suppression of Fusarium graminearium growth by differently structured starch types
AU - Svensson, Jan Tommy
AU - Olas, Justyna Jadwiga
AU - Skibior, Renata Jolanta
AU - Giese, Henriette
AU - Blennow, Per Gunnar Andreas
PY - 2012/7
Y1 - 2012/7
N2 - We investigated the growth behavior and amylolytic enzymes of Fusarium graminearum cultivated on different types of native starch granules including barley (A-type crystalline polymorph), potato and Curcuma zedoaria (B-type crystalline polymorph), cassava (C-type crystalline polymorph), and high AM maize (A + Vh-type crystalline polymorphs). F. graminearum grew poorly on B-type starches and the accumulation of biomass was similar to that obtained for fungi cultivated under carbohydrate starvation conditions. In comparison, three- to fivefold higher accumulation of fungal biomass was observed for growth on the A-, C- and A + Vh-type starches. Fungal glucoamylase and α-amylase activity increased over time in the presence of native starch granules. Interestingly, resistant B-type starches induced the highest amylolytic activity indicating that F. graminearum interacts with B-type granules although only limited degradation occur. Starch degradation products maltose and malto-oligosacharides was found to increase glucoamylase and α-amylase activity, whereas glucose acted as a catabolite repressor.
AB - We investigated the growth behavior and amylolytic enzymes of Fusarium graminearum cultivated on different types of native starch granules including barley (A-type crystalline polymorph), potato and Curcuma zedoaria (B-type crystalline polymorph), cassava (C-type crystalline polymorph), and high AM maize (A + Vh-type crystalline polymorphs). F. graminearum grew poorly on B-type starches and the accumulation of biomass was similar to that obtained for fungi cultivated under carbohydrate starvation conditions. In comparison, three- to fivefold higher accumulation of fungal biomass was observed for growth on the A-, C- and A + Vh-type starches. Fungal glucoamylase and α-amylase activity increased over time in the presence of native starch granules. Interestingly, resistant B-type starches induced the highest amylolytic activity indicating that F. graminearum interacts with B-type granules although only limited degradation occur. Starch degradation products maltose and malto-oligosacharides was found to increase glucoamylase and α-amylase activity, whereas glucose acted as a catabolite repressor.
U2 - 10.1002/star.201100120
DO - 10.1002/star.201100120
M3 - Journal article
SN - 0038-9056
VL - 64
SP - 563
EP - 571
JO - Starch
JF - Starch
IS - 7
ER -