Sunlight-induced DNA damage in human mononuclear cells

TY - JOUR

T1 - Sunlight-induced DNA damage in human mononuclear cells

AU - Møller, Peter

AU - Wallin, Hakan

AU - Holst, Erik

AU - Knudsen, Lisbeth E

N1 - Keywords: Adult; Cell Nucleus; DNA; DNA Damage; Environmental Exposure; Female; Humans; Leukocytes, Mononuclear; Life Style; Linear Models; Longitudinal Studies; Male; Middle Aged; Reproducibility of Results; Seasons; Sex Factors; Sunlight; Time Factors

PY - 2002

Y1 - 2002

N2 - In this study of 301 blood samples from 21 subjects, we found markedly higher levels of DNA damage (nonpyrimidine dimer types) in the summer than in the winter detected by single-cell gel electrophoresis. The level of DNA damage was influenced by the average daily influx of sunlight < 50 days prior to blood sampling. The 3 and 6 day periods before sampling influenced DNA damage the most. The importance of sunlight was further emphasized by a positive association of the DNA damage level to the amount of time the subjects had spent in the sun over a 3 day period prior to the sampling. The effect of sunlight was comparable to the interindividual variation, indicating that sunlight exposure and the individual's background were the two most important determinants for the basal level of DNA damage. Influence of other lifestyle factors such as exercise, intake of foods, infections, and age could not be detected. Our results suggest that sunlight penetrates the outer layer of the human epidermis and damages the DNA in mononuclear cells circulating in the vessels of the skin.

AB - In this study of 301 blood samples from 21 subjects, we found markedly higher levels of DNA damage (nonpyrimidine dimer types) in the summer than in the winter detected by single-cell gel electrophoresis. The level of DNA damage was influenced by the average daily influx of sunlight < 50 days prior to blood sampling. The 3 and 6 day periods before sampling influenced DNA damage the most. The importance of sunlight was further emphasized by a positive association of the DNA damage level to the amount of time the subjects had spent in the sun over a 3 day period prior to the sampling. The effect of sunlight was comparable to the interindividual variation, indicating that sunlight exposure and the individual's background were the two most important determinants for the basal level of DNA damage. Influence of other lifestyle factors such as exercise, intake of foods, infections, and age could not be detected. Our results suggest that sunlight penetrates the outer layer of the human epidermis and damages the DNA in mononuclear cells circulating in the vessels of the skin.

U2 - 10.1096/fj.01-0386com

DO - 10.1096/fj.01-0386com

M3 - Journal article

C2 - 11772935

SN - 0892-6638

VL - 16

SP - 45

EP - 53

JO - F A S E B Journal

JF - F A S E B Journal

IS - 1

ER -