Sucrose, glucose and fructose have similar genotoxicity in the rat colon and affect the metabolome

M. Hansen; D. Baunsgaard; Herman Autrup; Ulla Birgitte Vogel; Peter Møller; R. Lindecrona; Erik Håkan Richard Wallin; Henrik Enghusen Poulsen; Steffen Loft; Lars Ove Dragsted; M Hansen; Dorrit Baunsgaard; Herman Autrup; Ulla Birgitte Vogel; P Møller; R Lindecrona; Erik Håkan Richard Wallin; H E Poulsen; S Loft; L O Dragsted

doi:10.1016/j.fct.2007.09.110

Sucrose, glucose and fructose have similar genotoxicity in the rat colon and affect the metabolome

M. Hansen, D. Baunsgaard, Herman Autrup, Ulla Birgitte Vogel, Peter Møller, R. Lindecrona, Erik Håkan Richard Wallin, Henrik Enghusen Poulsen, Steffen Loft, Lars Ove Dragsted, M Hansen, Dorrit Baunsgaard, Herman Autrup, Ulla Birgitte Vogel, P Møller, R Lindecrona, Erik Håkan Richard Wallin, H E Poulsen, S Loft, L O Dragsted

12 Citations (Scopus)

Abstract

We have shown previously that a high sucrose intake increases the background level of somatic mutations and the level of bulky DNA adducts in the colon epithelium of rats. The mechanism may involve either glucose or fructose formed by hydrolysis of sucrose. Male Big Blue rats were fed 30% sucrose, glucose, fructose or potato starch as part of the diet. Mutation rates and bulky DNA adduct levels were determined in colon and liver. The concentration of short-chain fatty acids and pH were determined in caecum, C-peptide was determined in plasma, biomarkers for oxidative damage and proliferation were determined in colon, and a metabonomic analysis was performed in plasma and urine. The sugars increased the mutation rates in colon and the bulky adduct levels in colon and liver to a similar extent. All sugars decrease the caecal concentration of acetic acid and propionic acid. The metabonomic studies indicated disturbed amino acid metabolism and decrease in plasma and urinary acetate as a common feature for all sugars and confirmed triglyceridemic effects of fructose. In conclusion, the genotoxicity may be related to the altered chemical environment in the caecum and thereby also in the colon but we found no related changes in insulin resistance or oxidative stress.

Original language	English
Journal	Food and Chemical Toxicology
Volume	46
Issue number	2
Pages (from-to)	752-60
Number of pages	9
ISSN	0278-6915
DOIs	https://doi.org/10.1016/j.fct.2007.09.110 https://doi.org/10.1016/j.fct.2007.09.110
Publication status	Published - 1 Feb 2008

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Hansen, M., Baunsgaard, D., Autrup, H., Vogel, U. B., Møller, P., Lindecrona, R., Wallin, E. H. R., Poulsen, H. E., Loft, S., Dragsted, L. O., Hansen, M., Baunsgaard, D., Autrup, H., Vogel, U. B., Møller, P., Lindecrona, R., Wallin, E. H. R., Poulsen, H. E., Loft, S., & Dragsted, L. O. (2008). Sucrose, glucose and fructose have similar genotoxicity in the rat colon and affect the metabolome. Food and Chemical Toxicology, 46(2), 752-60. https://doi.org/10.1016/j.fct.2007.09.110, https://doi.org/10.1016/j.fct.2007.09.110

Hansen, M, Baunsgaard, D, Autrup, H, Vogel, UB, Møller, P, Lindecrona, R, Wallin, EHR, Poulsen, HE , Loft, S , Dragsted, LO, Hansen, M, Baunsgaard, D, Autrup, H, Vogel, UB, Møller, P, Lindecrona, R, Wallin, EHR, Poulsen, HE, Loft, S & Dragsted, LO 2008, 'Sucrose, glucose and fructose have similar genotoxicity in the rat colon and affect the metabolome', Food and Chemical Toxicology, vol. 46, no. 2, pp. 752-60. https://doi.org/10.1016/j.fct.2007.09.110, https://doi.org/10.1016/j.fct.2007.09.110

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title = "Sucrose, glucose and fructose have similar genotoxicity in the rat colon and affect the metabolome",

abstract = "We have shown previously that a high sucrose intake increases the background level of somatic mutations and the level of bulky DNA adducts in the colon epithelium of rats. The mechanism may involve either glucose or fructose formed by hydrolysis of sucrose. Male Big Blue rats were fed 30% sucrose, glucose, fructose or potato starch as part of the diet. Mutation rates and bulky DNA adduct levels were determined in colon and liver. The concentration of short-chain fatty acids and pH were determined in caecum, C-peptide was determined in plasma, biomarkers for oxidative damage and proliferation were determined in colon, and a metabonomic analysis was performed in plasma and urine. The sugars increased the mutation rates in colon and the bulky adduct levels in colon and liver to a similar extent. All sugars decrease the caecal concentration of acetic acid and propionic acid. The metabonomic studies indicated disturbed amino acid metabolism and decrease in plasma and urinary acetate as a common feature for all sugars and confirmed triglyceridemic effects of fructose. In conclusion, the genotoxicity may be related to the altered chemical environment in the caecum and thereby also in the colon but we found no related changes in insulin resistance or oxidative stress.",

author = "M. Hansen and D. Baunsgaard and Herman Autrup and Vogel, {Ulla Birgitte} and Peter M{\o}ller and R. Lindecrona and Wallin, {Erik H{\aa}kan Richard} and Poulsen, {Henrik Enghusen} and Steffen Loft and Dragsted, {Lars Ove} and M Hansen and Dorrit Baunsgaard and Herman Autrup and Vogel, {Ulla Birgitte} and P M{\o}ller and R Lindecrona and Wallin, {Erik H{\aa}kan Richard} and Poulsen, {H E} and S Loft and Dragsted, {L O}",

note = "Keywords: Animals; Colon; DNA Damage; Fructose; Glucose; Magnetic Resonance Spectroscopy; Male; Mutagenicity Tests; Mutation; Organ Size; Rats; Sucrose; Sweetening Agents",

year = "2008",

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doi = "10.1016/j.fct.2007.09.110",

language = "English",

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T1 - Sucrose, glucose and fructose have similar genotoxicity in the rat colon and affect the metabolome

AU - Hansen, M.

AU - Baunsgaard, D.

AU - Autrup, Herman

AU - Vogel, Ulla Birgitte

AU - Møller, Peter

AU - Lindecrona, R.

AU - Wallin, Erik Håkan Richard

AU - Poulsen, Henrik Enghusen

AU - Loft, Steffen

AU - Dragsted, Lars Ove

AU - Hansen, M

AU - Baunsgaard, Dorrit

AU - Autrup, Herman

AU - Vogel, Ulla Birgitte

AU - Møller, P

AU - Lindecrona, R

AU - Wallin, Erik Håkan Richard

AU - Poulsen, H E

AU - Loft, S

AU - Dragsted, L O

N1 - Keywords: Animals; Colon; DNA Damage; Fructose; Glucose; Magnetic Resonance Spectroscopy; Male; Mutagenicity Tests; Mutation; Organ Size; Rats; Sucrose; Sweetening Agents

PY - 2008/2/1

Y1 - 2008/2/1

N2 - We have shown previously that a high sucrose intake increases the background level of somatic mutations and the level of bulky DNA adducts in the colon epithelium of rats. The mechanism may involve either glucose or fructose formed by hydrolysis of sucrose. Male Big Blue rats were fed 30% sucrose, glucose, fructose or potato starch as part of the diet. Mutation rates and bulky DNA adduct levels were determined in colon and liver. The concentration of short-chain fatty acids and pH were determined in caecum, C-peptide was determined in plasma, biomarkers for oxidative damage and proliferation were determined in colon, and a metabonomic analysis was performed in plasma and urine. The sugars increased the mutation rates in colon and the bulky adduct levels in colon and liver to a similar extent. All sugars decrease the caecal concentration of acetic acid and propionic acid. The metabonomic studies indicated disturbed amino acid metabolism and decrease in plasma and urinary acetate as a common feature for all sugars and confirmed triglyceridemic effects of fructose. In conclusion, the genotoxicity may be related to the altered chemical environment in the caecum and thereby also in the colon but we found no related changes in insulin resistance or oxidative stress.

AB - We have shown previously that a high sucrose intake increases the background level of somatic mutations and the level of bulky DNA adducts in the colon epithelium of rats. The mechanism may involve either glucose or fructose formed by hydrolysis of sucrose. Male Big Blue rats were fed 30% sucrose, glucose, fructose or potato starch as part of the diet. Mutation rates and bulky DNA adduct levels were determined in colon and liver. The concentration of short-chain fatty acids and pH were determined in caecum, C-peptide was determined in plasma, biomarkers for oxidative damage and proliferation were determined in colon, and a metabonomic analysis was performed in plasma and urine. The sugars increased the mutation rates in colon and the bulky adduct levels in colon and liver to a similar extent. All sugars decrease the caecal concentration of acetic acid and propionic acid. The metabonomic studies indicated disturbed amino acid metabolism and decrease in plasma and urinary acetate as a common feature for all sugars and confirmed triglyceridemic effects of fructose. In conclusion, the genotoxicity may be related to the altered chemical environment in the caecum and thereby also in the colon but we found no related changes in insulin resistance or oxidative stress.

U2 - 10.1016/j.fct.2007.09.110

DO - 10.1016/j.fct.2007.09.110

M3 - Journal article

C2 - 17988776

SN - 0278-6915

VL - 46

SP - 752

EP - 760

JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

IS - 2

ER -

Sucrose, glucose and fructose have similar genotoxicity in the rat colon and affect the metabolome

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