Abstract
Immunoreactive substance P and neurokinin A were measured with radioimmunoassay in extracts of different segments of porcine gastrointestinal tract using C-terminally directed antisera. In all segments, the concentrations of substance P and neurokinin A were similar. The largest concentrations of both peptides were found in the mid-colon. By gel chromatography and reversed-phase high pressure liquid chromatography the immunoreactivity in extracts from ileum eluted as homogenous peptides at the positions of synthetic substance P and neurokinin A, respectively. No neurokinin B was found. By immunohistochemistry of porcine duodenum, jejunum, ileum and mid-colon, identical localization patterns were found for substance P and neurokinin A, and the two peptides demonstrated by double immunofluorescence to be colocalized in the enteric nervous system of the ileum. We conclude that the tachykinins substance P and neurokinin A are codistributed and colocalized in the procine gastrointestinal tract and suggest that the two peptides are produced from a common precursor, beta- and/or gamma-preprotachykinin, in the same neurons.
| Original language | English |
|---|---|
| Journal | Peptides |
| Volume | 12 |
| Issue number | 5 |
| Pages (from-to) | 963-73 |
| Number of pages | 11 |
| ISSN | 0196-9781 |
| DOIs | |
| Publication status | Published - 1991 |
Keywords
- Animals
- Digestive System
- Fluorescent Antibody Technique
- Immune Sera
- Muscle, Smooth
- Myenteric Plexus
- Nerve Fibers
- Neurokinin A
- Organ Specificity
- Substance P
- Swine