Studies of patulin diffusion in apple using a gfp transformant of Penicillium expansum

Birgit Jensen; Per Væggemose Nielsen; Dan Funck Jensen; Kristian Fog Nielsen

Studies of patulin diffusion in apple using a gfp transformant of Penicillium expansum

Birgit Jensen, Per Væggemose Nielsen, Dan Funck Jensen, Kristian Fog Nielsen

Abstract

To study whether patulin diffuses in front of the pathogen or vice versa, we have inserted a constitutively expressed gfp-construct into Penicillium expansum. Spores and hyphal growth of the transformant IBT21525gfp4 in apple tissue was visualized directly by fluorescence mocroscopy. Using HPLC/UV, patulin was detected in apparently sound tissue more than 2 cm from the edge of lesions caused by P. expansum wildtypes.

Original language	English
Publication date	2006
Number of pages	3
Publication status	Published - 2006
Event	COST Action 864 : Pome Fruit Health Research in Europe, Current Status 2006 - Vienna, Austria Duration: 20 Nov 2006 → 21 Nov 2006

Conference

Conference	COST Action 864 : Pome Fruit Health Research in Europe, Current Status 2006
Country/Territory	Austria
City	Vienna
Period	20/11/2006 → 21/11/2006

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title = "Studies of patulin diffusion in apple using a gfp transformant of Penicillium expansum",

abstract = "To study whether patulin diffuses in front of the pathogen or vice versa, we have inserted a constitutively expressed gfp-construct into Penicillium expansum. Spores and hyphal growth of the transformant IBT21525gfp4 in apple tissue was visualized directly by fluorescence mocroscopy. Using HPLC/UV, patulin was detected in apparently sound tissue more than 2 cm from the edge of lesions caused by P. expansum wildtypes.",

author = "Birgit Jensen and Nielsen, {Per V{\ae}ggemose} and Jensen, {Dan Funck} and Nielsen, {Kristian Fog}",

note = "Sider: 129-131; COST Action 864 : Pome Fruit Health Research in Europe, Current Status 2006 ; Conference date: 20-11-2006 Through 21-11-2006",

year = "2006",

language = "English",

}

TY - ABST

T1 - Studies of patulin diffusion in apple using a gfp transformant of Penicillium expansum

AU - Jensen, Birgit

AU - Nielsen, Per Væggemose

AU - Jensen, Dan Funck

AU - Nielsen, Kristian Fog

N1 - Sider: 129-131

PY - 2006

Y1 - 2006

N2 - To study whether patulin diffuses in front of the pathogen or vice versa, we have inserted a constitutively expressed gfp-construct into Penicillium expansum. Spores and hyphal growth of the transformant IBT21525gfp4 in apple tissue was visualized directly by fluorescence mocroscopy. Using HPLC/UV, patulin was detected in apparently sound tissue more than 2 cm from the edge of lesions caused by P. expansum wildtypes.

AB - To study whether patulin diffuses in front of the pathogen or vice versa, we have inserted a constitutively expressed gfp-construct into Penicillium expansum. Spores and hyphal growth of the transformant IBT21525gfp4 in apple tissue was visualized directly by fluorescence mocroscopy. Using HPLC/UV, patulin was detected in apparently sound tissue more than 2 cm from the edge of lesions caused by P. expansum wildtypes.

M3 - Conference abstract for conference

T2 - COST Action 864 : Pome Fruit Health Research in Europe, Current Status 2006

Y2 - 20 November 2006 through 21 November 2006

ER -

Studies of patulin diffusion in apple using a gfp transformant of Penicillium expansum

Abstract

Conference

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