Studies of patulin diffusion in apple using a gfp transformant of Penicillium expansum

TY - ABST

T1 - Studies of patulin diffusion in apple using a gfp transformant of Penicillium expansum

AU - Jensen, Birgit

AU - Nielsen, Per Væggemose

AU - Jensen, Dan Funck

AU - Nielsen, Kristian Fog

N1 - Sider: 129-131

PY - 2006

Y1 - 2006

N2 - To study whether patulin diffuses in front of the pathogen or vice versa, we have inserted a constitutively expressed gfp-construct into Penicillium expansum. Spores and hyphal growth of the transformant IBT21525gfp4 in apple tissue was visualized directly by fluorescence mocroscopy. Using HPLC/UV, patulin was detected in apparently sound tissue more than 2 cm from the edge of lesions caused by P. expansum wildtypes.

AB - To study whether patulin diffuses in front of the pathogen or vice versa, we have inserted a constitutively expressed gfp-construct into Penicillium expansum. Spores and hyphal growth of the transformant IBT21525gfp4 in apple tissue was visualized directly by fluorescence mocroscopy. Using HPLC/UV, patulin was detected in apparently sound tissue more than 2 cm from the edge of lesions caused by P. expansum wildtypes.

M3 - Conference abstract for conference

T2 - COST Action 864 : Pome Fruit Health Research in Europe, Current Status 2006

Y2 - 20 November 2006 through 21 November 2006

ER -