Spectral components of cytosolic [Ca2+] spiking in neurons

J Kardos; N Szilágyi; G Juhász; B Belhage; A Schousboe

Spectral components of cytosolic [Ca2+] spiking in neurons

J Kardos, N Szilágyi, G Juhász, B Belhage, A Schousboe

5 Citations (Scopus)

Abstract

We show here, by means of evolutionary spectral analysis and synthesis of cytosolic Ca2+ ([Ca2+]c) spiking observed at the single cell level using digital imaging fluorescence microscopy of fura-2-loaded mouse cerebellar granule cells in culture, that [Ca2+]c spiking can be resolved into evolutionary spectra of a characteristic set of frequencies. Non-delayed small spikes on top of sustained [Ca2+]c were synthesized by a main component frequency, 0.132+/-0.012 Hz, showing its maximal amplitude in phase with the start of depolarization (25 mM KCI) combined with caffeine (10 mM) application. Delayed complex responses of large [Ca2+]c spiking observed in cells from a different set of cultures were synthesized by a set of frequencies within the range 0.018-0.117 Hz. Differential frequency patterns are suggested as characteristics of the [Ca2+]c spiking responses of neurons under different conditions.

Original language	English
Journal	NeuroReport
Volume	9
Issue number	4
Pages (from-to)	721-4
Number of pages	4
ISSN	0959-4965
Publication status	Published - 1998

Cite this

@article{ef23ba9043d711df928f000ea68e967b,

title = "Spectral components of cytosolic [Ca2+] spiking in neurons",

abstract = "We show here, by means of evolutionary spectral analysis and synthesis of cytosolic Ca2+ ([Ca2+]c) spiking observed at the single cell level using digital imaging fluorescence microscopy of fura-2-loaded mouse cerebellar granule cells in culture, that [Ca2+]c spiking can be resolved into evolutionary spectra of a characteristic set of frequencies. Non-delayed small spikes on top of sustained [Ca2+]c were synthesized by a main component frequency, 0.132+/-0.012 Hz, showing its maximal amplitude in phase with the start of depolarization (25 mM KCI) combined with caffeine (10 mM) application. Delayed complex responses of large [Ca2+]c spiking observed in cells from a different set of cultures were synthesized by a set of frequencies within the range 0.018-0.117 Hz. Differential frequency patterns are suggested as characteristics of the [Ca2+]c spiking responses of neurons under different conditions.",

author = "J Kardos and N Szil{\'a}gyi and G Juh{\'a}sz and B Belhage and A Schousboe",

note = "Keywords: Animals; Caffeine; Calcium; Cells, Cultured; Cerebellum; Cytosol; Evoked Potentials; Kinetics; Mice; Neurons; Time Factors",

year = "1998",

language = "English",

volume = "9",

pages = "721--4",

journal = "NeuroReport",

issn = "0959-4965",

publisher = "Lippincott Williams & Wilkins",

number = "4",

}

TY - JOUR

T1 - Spectral components of cytosolic [Ca2+] spiking in neurons

AU - Kardos, J

AU - Szilágyi, N

AU - Juhász, G

AU - Belhage, B

AU - Schousboe, A

N1 - Keywords: Animals; Caffeine; Calcium; Cells, Cultured; Cerebellum; Cytosol; Evoked Potentials; Kinetics; Mice; Neurons; Time Factors

PY - 1998

Y1 - 1998

N2 - We show here, by means of evolutionary spectral analysis and synthesis of cytosolic Ca2+ ([Ca2+]c) spiking observed at the single cell level using digital imaging fluorescence microscopy of fura-2-loaded mouse cerebellar granule cells in culture, that [Ca2+]c spiking can be resolved into evolutionary spectra of a characteristic set of frequencies. Non-delayed small spikes on top of sustained [Ca2+]c were synthesized by a main component frequency, 0.132+/-0.012 Hz, showing its maximal amplitude in phase with the start of depolarization (25 mM KCI) combined with caffeine (10 mM) application. Delayed complex responses of large [Ca2+]c spiking observed in cells from a different set of cultures were synthesized by a set of frequencies within the range 0.018-0.117 Hz. Differential frequency patterns are suggested as characteristics of the [Ca2+]c spiking responses of neurons under different conditions.

AB - We show here, by means of evolutionary spectral analysis and synthesis of cytosolic Ca2+ ([Ca2+]c) spiking observed at the single cell level using digital imaging fluorescence microscopy of fura-2-loaded mouse cerebellar granule cells in culture, that [Ca2+]c spiking can be resolved into evolutionary spectra of a characteristic set of frequencies. Non-delayed small spikes on top of sustained [Ca2+]c were synthesized by a main component frequency, 0.132+/-0.012 Hz, showing its maximal amplitude in phase with the start of depolarization (25 mM KCI) combined with caffeine (10 mM) application. Delayed complex responses of large [Ca2+]c spiking observed in cells from a different set of cultures were synthesized by a set of frequencies within the range 0.018-0.117 Hz. Differential frequency patterns are suggested as characteristics of the [Ca2+]c spiking responses of neurons under different conditions.

M3 - Journal article

C2 - 9559945

SN - 0959-4965

VL - 9

SP - 721

EP - 724

JO - NeuroReport

JF - NeuroReport

IS - 4

ER -

Spectral components of cytosolic [Ca2+] spiking in neurons

Abstract

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