@article{ef23ba9043d711df928f000ea68e967b,
title = "Spectral components of cytosolic [Ca2+] spiking in neurons",
abstract = "We show here, by means of evolutionary spectral analysis and synthesis of cytosolic Ca2+ ([Ca2+]c) spiking observed at the single cell level using digital imaging fluorescence microscopy of fura-2-loaded mouse cerebellar granule cells in culture, that [Ca2+]c spiking can be resolved into evolutionary spectra of a characteristic set of frequencies. Non-delayed small spikes on top of sustained [Ca2+]c were synthesized by a main component frequency, 0.132+/-0.012 Hz, showing its maximal amplitude in phase with the start of depolarization (25 mM KCI) combined with caffeine (10 mM) application. Delayed complex responses of large [Ca2+]c spiking observed in cells from a different set of cultures were synthesized by a set of frequencies within the range 0.018-0.117 Hz. Differential frequency patterns are suggested as characteristics of the [Ca2+]c spiking responses of neurons under different conditions.",
author = "J Kardos and N Szil{\'a}gyi and G Juh{\'a}sz and B Belhage and A Schousboe",
note = "Keywords: Animals; Caffeine; Calcium; Cells, Cultured; Cerebellum; Cytosol; Evoked Potentials; Kinetics; Mice; Neurons; Time Factors",
year = "1998",
language = "English",
volume = "9",
pages = "721--4",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams & Wilkins",
number = "4",
}