Specificity of anti-citrullinated protein antibodies in rheumatoid arthritis

Nicole Trier; Bettina Eide Holm; Paul Robert Hansen; Ole Slot; Henning  Locht; Gunnar Houen

doi:doi:10.3390/antib8020037

Specificity of anti-citrullinated protein antibodies in rheumatoid arthritis

Nicole Trier, Bettina Eide Holm, Paul Robert Hansen, Ole Slot, Henning Locht, Gunnar Houen

6 Downloads (Pure)

Abstract

Rheumatoid arthritis (RA) is an autoimmune disease of unknown etiology. The majority of individuals with RA are positive for the disease-specific anti-citrullinated protein antibodies (ACPAs). These antibodies are primarily of cross-reactive nature, hence, the true autoantigen to ACPA remains unidentified. In this study, we analyzed the reactivity of RA sera to several post-translationally modified epitopes, in order to further characterize the specific nature of ACPAs by immunoassays. Substituting citrulline with other amino acids, e.g., D-citrulline, homo-citrulline and methyl-arginine illustrated that ACPAs are utmost specific for citrullinated targets. Collectively, these findings support that ACPAs and citrullinated targets are specific for RA, making citrulline-containing peptide targets the most effective assays for detection of ACPAs.

Original language	English
Article number	37
Journal	Antibodies
Volume	8
Issue number	2
Number of pages	10
ISSN	2073-4468
DOIs	https://doi.org/doi:10.3390/antib8020037
Publication status	Published - Jun 2019

Access to Document

doi:10.3390/antib8020037Licence: CC BY

antibodies-08-00037-v3Final published version, 790 KBLicence: CC BY

Cite this

@article{a382791aa26f402aa20b36f27ea4d13e,

title = "Specificity of anti-citrullinated protein antibodies in rheumatoid arthritis",

abstract = "Rheumatoid arthritis (RA) is an autoimmune disease of unknown etiology. The majority of individuals with RA are positive for the disease-specific anti-citrullinated protein antibodies (ACPAs). These antibodies are primarily of cross-reactive nature, hence, the true autoantigen to ACPA remains unidentified. In this study, we analyzed the reactivity of RA sera to several post-translationally modified epitopes, in order to further characterize the specific nature of ACPAs by immunoassays. Substituting citrulline with other amino acids, e.g., D-citrulline, homo-citrulline and methyl-arginine illustrated that ACPAs are utmost specific for citrullinated targets. Collectively, these findings support that ACPAs and citrullinated targets are specific for RA, making citrulline-containing peptide targets the most effective assays for detection of ACPAs.",

author = "Nicole Trier and Holm, {Bettina Eide} and Hansen, {Paul Robert} and Ole Slot and Henning Locht and Gunnar Houen",

year = "2019",

month = jun,

doi = "doi:10.3390/antib8020037",

language = "English",

volume = "8",

journal = "Antibodies",

issn = "2073-4468",

publisher = "M D P I AG",

number = "2",

}

TY - JOUR

T1 - Specificity of anti-citrullinated protein antibodies in rheumatoid arthritis

AU - Trier, Nicole

AU - Holm, Bettina Eide

AU - Hansen, Paul Robert

AU - Slot, Ole

AU - Locht, Henning

AU - Houen, Gunnar

PY - 2019/6

Y1 - 2019/6

N2 - Rheumatoid arthritis (RA) is an autoimmune disease of unknown etiology. The majority of individuals with RA are positive for the disease-specific anti-citrullinated protein antibodies (ACPAs). These antibodies are primarily of cross-reactive nature, hence, the true autoantigen to ACPA remains unidentified. In this study, we analyzed the reactivity of RA sera to several post-translationally modified epitopes, in order to further characterize the specific nature of ACPAs by immunoassays. Substituting citrulline with other amino acids, e.g., D-citrulline, homo-citrulline and methyl-arginine illustrated that ACPAs are utmost specific for citrullinated targets. Collectively, these findings support that ACPAs and citrullinated targets are specific for RA, making citrulline-containing peptide targets the most effective assays for detection of ACPAs.

AB - Rheumatoid arthritis (RA) is an autoimmune disease of unknown etiology. The majority of individuals with RA are positive for the disease-specific anti-citrullinated protein antibodies (ACPAs). These antibodies are primarily of cross-reactive nature, hence, the true autoantigen to ACPA remains unidentified. In this study, we analyzed the reactivity of RA sera to several post-translationally modified epitopes, in order to further characterize the specific nature of ACPAs by immunoassays. Substituting citrulline with other amino acids, e.g., D-citrulline, homo-citrulline and methyl-arginine illustrated that ACPAs are utmost specific for citrullinated targets. Collectively, these findings support that ACPAs and citrullinated targets are specific for RA, making citrulline-containing peptide targets the most effective assays for detection of ACPAs.

U2 - doi:10.3390/antib8020037

DO - doi:10.3390/antib8020037

M3 - Journal article

SN - 2073-4468

VL - 8

JO - Antibodies

JF - Antibodies

IS - 2

M1 - 37

ER -

Specificity of anti-citrullinated protein antibodies in rheumatoid arthritis

Abstract

Access to Document

Fingerprint

Cite this