Specificity of anti-citrullinated protein antibodies in rheumatoid arthritis

Nicole Trier, Bettina Eide Holm, Paul Robert Hansen, Ole Slot, Henning Locht, Gunnar Houen

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    Abstract

    Rheumatoid arthritis (RA) is an autoimmune disease of unknown etiology. The majority of individuals with RA are positive for the disease-specific anti-citrullinated protein antibodies (ACPAs). These antibodies are primarily of cross-reactive nature, hence, the true autoantigen to ACPA remains unidentified. In this study, we analyzed the reactivity of RA sera to several post-translationally modified epitopes, in order to further characterize the specific nature of ACPAs by immunoassays. Substituting citrulline with other amino acids, e.g., D-citrulline, homo-citrulline and methyl-arginine illustrated that ACPAs are utmost specific for citrullinated targets. Collectively, these findings support that ACPAs and citrullinated targets are specific for RA, making citrulline-containing peptide targets the most effective assays for detection of ACPAs.

    OriginalsprogEngelsk
    Artikelnummer37
    TidsskriftAntibodies
    Vol/bind8
    Udgave nummer2
    Antal sider10
    ISSN2073-4468
    DOI
    StatusUdgivet - jun. 2019

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