Specific depletion of mature T lymphocytes from human bone marrow

C Geisler; J Møller; T Plesner; E Dickmeiss; G Pallesen; J K Larsen; N Jacobsen; A Svejgaard

Specific depletion of mature T lymphocytes from human bone marrow

C Geisler, J Møller, T Plesner, E Dickmeiss, G Pallesen, J K Larsen, N Jacobsen, A Svejgaard

Department of Immunology and Microbiology

4 Citations (Scopus)

Abstract

An effective method for specific depletion of mature T lymphocytes from human bone marrow mononuclear cells (BMMC) with preservation of prethymic T cells and natural killer (NK) cells is presented. The BMMC were incubated with F101.01, a monoclonal antibody recognizing an epitope of the T-cell receptor-CD3 complex, and subsequently with immunomagnetic beads. Flow cytometric analysis demonstrated that mature T cells were efficiently depleted and that NK cells and prethymic T cells were preserved in the BMMC. Furthermore, T cell-mediated immune reaction as measured by thymidine incorporation after stimulation with phytohaemagglutinin was abolished, whereas NK activity as measured by 51Cr release using K562 as target cells was preserved. Recovery of colony-forming units of granulocyte-macrophages was 60-70%.

Original language	English
Journal	Scandinavian Journal of Immunology
Volume	29
Issue number	5
Pages (from-to)	617-25
Number of pages	8
ISSN	0300-9475
Publication status	Published - 1989

Cite this

@article{fc36ff80b0a811ddb538000ea68e967b,

title = "Specific depletion of mature T lymphocytes from human bone marrow",

abstract = "An effective method for specific depletion of mature T lymphocytes from human bone marrow mononuclear cells (BMMC) with preservation of prethymic T cells and natural killer (NK) cells is presented. The BMMC were incubated with F101.01, a monoclonal antibody recognizing an epitope of the T-cell receptor-CD3 complex, and subsequently with immunomagnetic beads. Flow cytometric analysis demonstrated that mature T cells were efficiently depleted and that NK cells and prethymic T cells were preserved in the BMMC. Furthermore, T cell-mediated immune reaction as measured by thymidine incorporation after stimulation with phytohaemagglutinin was abolished, whereas NK activity as measured by 51Cr release using K562 as target cells was preserved. Recovery of colony-forming units of granulocyte-macrophages was 60-70%.",

author = "C Geisler and J M{\o}ller and T Plesner and E Dickmeiss and G Pallesen and Larsen, {J K} and N Jacobsen and A Svejgaard",

note = "Keywords: Bone Marrow; Bone Marrow Cells; Cell Separation; Colony-Forming Units Assay; Flow Cytometry; Hematopoietic Stem Cells; Humans; Killer Cells, Natural; Leukocyte Count; Lymphocyte Activation; Lymphocyte Depletion; T-Lymphocytes",

year = "1989",

language = "English",

volume = "29",

pages = "617--25",

journal = "Scandinavian Journal of Immunology, Supplement",

issn = "0301-6323",

publisher = "Wiley-Blackwell",

number = "5",

}

TY - JOUR

T1 - Specific depletion of mature T lymphocytes from human bone marrow

AU - Geisler, C

AU - Møller, J

AU - Plesner, T

AU - Dickmeiss, E

AU - Pallesen, G

AU - Larsen, J K

AU - Jacobsen, N

AU - Svejgaard, A

N1 - Keywords: Bone Marrow; Bone Marrow Cells; Cell Separation; Colony-Forming Units Assay; Flow Cytometry; Hematopoietic Stem Cells; Humans; Killer Cells, Natural; Leukocyte Count; Lymphocyte Activation; Lymphocyte Depletion; T-Lymphocytes

PY - 1989

Y1 - 1989

N2 - An effective method for specific depletion of mature T lymphocytes from human bone marrow mononuclear cells (BMMC) with preservation of prethymic T cells and natural killer (NK) cells is presented. The BMMC were incubated with F101.01, a monoclonal antibody recognizing an epitope of the T-cell receptor-CD3 complex, and subsequently with immunomagnetic beads. Flow cytometric analysis demonstrated that mature T cells were efficiently depleted and that NK cells and prethymic T cells were preserved in the BMMC. Furthermore, T cell-mediated immune reaction as measured by thymidine incorporation after stimulation with phytohaemagglutinin was abolished, whereas NK activity as measured by 51Cr release using K562 as target cells was preserved. Recovery of colony-forming units of granulocyte-macrophages was 60-70%.

AB - An effective method for specific depletion of mature T lymphocytes from human bone marrow mononuclear cells (BMMC) with preservation of prethymic T cells and natural killer (NK) cells is presented. The BMMC were incubated with F101.01, a monoclonal antibody recognizing an epitope of the T-cell receptor-CD3 complex, and subsequently with immunomagnetic beads. Flow cytometric analysis demonstrated that mature T cells were efficiently depleted and that NK cells and prethymic T cells were preserved in the BMMC. Furthermore, T cell-mediated immune reaction as measured by thymidine incorporation after stimulation with phytohaemagglutinin was abolished, whereas NK activity as measured by 51Cr release using K562 as target cells was preserved. Recovery of colony-forming units of granulocyte-macrophages was 60-70%.

M3 - Journal article

C2 - 2786245

SN - 0301-6323

VL - 29

SP - 617

EP - 625

JO - Scandinavian Journal of Immunology, Supplement

JF - Scandinavian Journal of Immunology, Supplement

IS - 5

ER -

Specific depletion of mature T lymphocytes from human bone marrow

Abstract

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