Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

Michael Buckley; Matthew Collins; Jane Thomas-Oaies; Julie C. Wilson

doi:10.1002/rcm.4316

Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

Michael Buckley^*, Matthew Collins, Jane Thomas-Oaies, Julie C. Wilson

^*Corresponding author for this work

278 Citations (Scopus)

Abstract

Species identification of fragmentary bone, such as in rendered meat and bone meal or from archaeological sites, is often difficult in the absence of clear morphological markers. Here we present a robust method of analysing genus-specific collagen peptides by mass spectrometry simply by using solid-phase extraction (a C18 ZipTip® for peptide purification, rather than liquid chromatography/ mass spectrometry (LC/MS). Analysis of the collagen from 32 different mammal species identified a total of 92 peptide markers that could be used for species identification, for example, in processed food and animal feed. A set of ancient (>100 ka@10°C) bone samples was also analysed to show that the proposed method has applications to archaeological bone identification.

Original language	English
Journal	Rapid Communications in Mass Spectrometry
Volume	23
Issue number	23
Pages (from-to)	3843-3854
Number of pages	12
ISSN	0951-4198
DOIs	https://doi.org/10.1002/rcm.4316
Publication status	Published - 2009
Externally published	Yes

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10.1002/rcm.4316

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Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. / Buckley, Michael; Collins, Matthew; Thomas-Oaies, Jane et al.
In: Rapid Communications in Mass Spectrometry, Vol. 23, No. 23, 2009, p. 3843-3854.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry",

abstract = "Species identification of fragmentary bone, such as in rendered meat and bone meal or from archaeological sites, is often difficult in the absence of clear morphological markers. Here we present a robust method of analysing genus-specific collagen peptides by mass spectrometry simply by using solid-phase extraction (a C18 ZipTip{\textregistered} for peptide purification, rather than liquid chromatography/ mass spectrometry (LC/MS). Analysis of the collagen from 32 different mammal species identified a total of 92 peptide markers that could be used for species identification, for example, in processed food and animal feed. A set of ancient (>100 ka@10°C) bone samples was also analysed to show that the proposed method has applications to archaeological bone identification.",

author = "Michael Buckley and Matthew Collins and Jane Thomas-Oaies and Wilson, {Julie C.}",

note = "Erratum: Species identification by analysis of bone collagen using matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry DOI: 10.1002/rcm.4745",

year = "2009",

doi = "10.1002/rcm.4316",

language = "English",

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journal = "Rapid Communications in Mass Spectrometry",

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T1 - Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

AU - Buckley, Michael

AU - Collins, Matthew

AU - Thomas-Oaies, Jane

AU - Wilson, Julie C.

N1 - Erratum: Species identification by analysis of bone collagen using matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry DOI: 10.1002/rcm.4745

PY - 2009

Y1 - 2009

N2 - Species identification of fragmentary bone, such as in rendered meat and bone meal or from archaeological sites, is often difficult in the absence of clear morphological markers. Here we present a robust method of analysing genus-specific collagen peptides by mass spectrometry simply by using solid-phase extraction (a C18 ZipTip® for peptide purification, rather than liquid chromatography/ mass spectrometry (LC/MS). Analysis of the collagen from 32 different mammal species identified a total of 92 peptide markers that could be used for species identification, for example, in processed food and animal feed. A set of ancient (>100 ka@10°C) bone samples was also analysed to show that the proposed method has applications to archaeological bone identification.

AB - Species identification of fragmentary bone, such as in rendered meat and bone meal or from archaeological sites, is often difficult in the absence of clear morphological markers. Here we present a robust method of analysing genus-specific collagen peptides by mass spectrometry simply by using solid-phase extraction (a C18 ZipTip® for peptide purification, rather than liquid chromatography/ mass spectrometry (LC/MS). Analysis of the collagen from 32 different mammal species identified a total of 92 peptide markers that could be used for species identification, for example, in processed food and animal feed. A set of ancient (>100 ka@10°C) bone samples was also analysed to show that the proposed method has applications to archaeological bone identification.

UR - https://doi.org/10.1002/rcm.4745

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DO - 10.1002/rcm.4316

M3 - Journal article

C2 - 19899187

AN - SCOPUS:71949102106

SN - 0951-4198

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SP - 3843

EP - 3854

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

IS - 23

ER -

Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

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