Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

TY - JOUR

T1 - Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

AU - Buckley, Michael

AU - Collins, Matthew

AU - Thomas-Oaies, Jane

AU - Wilson, Julie C.

N1 - Erratum: Species identification by analysis of bone collagen using matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry DOI: 10.1002/rcm.4745

PY - 2009

Y1 - 2009

N2 - Species identification of fragmentary bone, such as in rendered meat and bone meal or from archaeological sites, is often difficult in the absence of clear morphological markers. Here we present a robust method of analysing genus-specific collagen peptides by mass spectrometry simply by using solid-phase extraction (a C18 ZipTip® for peptide purification, rather than liquid chromatography/ mass spectrometry (LC/MS). Analysis of the collagen from 32 different mammal species identified a total of 92 peptide markers that could be used for species identification, for example, in processed food and animal feed. A set of ancient (>100 ka@10°C) bone samples was also analysed to show that the proposed method has applications to archaeological bone identification.

AB - Species identification of fragmentary bone, such as in rendered meat and bone meal or from archaeological sites, is often difficult in the absence of clear morphological markers. Here we present a robust method of analysing genus-specific collagen peptides by mass spectrometry simply by using solid-phase extraction (a C18 ZipTip® for peptide purification, rather than liquid chromatography/ mass spectrometry (LC/MS). Analysis of the collagen from 32 different mammal species identified a total of 92 peptide markers that could be used for species identification, for example, in processed food and animal feed. A set of ancient (>100 ka@10°C) bone samples was also analysed to show that the proposed method has applications to archaeological bone identification.

UR - https://doi.org/10.1002/rcm.4745

U2 - 10.1002/rcm.4316

DO - 10.1002/rcm.4316

M3 - Journal article

C2 - 19899187

AN - SCOPUS:71949102106

SN - 0951-4198

VL - 23

SP - 3843

EP - 3854

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

IS - 23

ER -