Sox2-positive dermal papilla cells specify hair follicle type in mammalian epidermis

TY - JOUR

T1 - Sox2-positive dermal papilla cells specify hair follicle type in mammalian epidermis

AU - Driskell, Ryan R

AU - Giangreco, Adam

AU - Jensen, Kim B

AU - Mulder, Klaas W

AU - Watt, Fiona M

PY - 2009/8

Y1 - 2009/8

N2 - The dermal papilla comprises the specialised mesenchymal cells at the base of the hair follicle. Communication between dermal papilla cells and the overlying epithelium is essential for differentiation of the hair follicle lineages. We report that Sox2 is expressed in all dermal papillae at E16.5, but from E18.5 onwards expression is confined to a subset of dermal papillae. In postnatal skin, Sox2 is only expressed in the dermal papillae of guard/awl/auchene follicles, whereas CD133 is expressed both in guard/awl/auchene and in zigzag dermal papillae. Using transgenic mice that express GFP under the control of the Sox2 promoter, we isolated Sox2(+) (GFP(+)) CD133(+) cells and compared them with Sox2(-) (GFP(-)) CD133(+) dermal papilla cells. In addition to the 'core' dermal papilla gene signature, each subpopulation expressed distinct sets of genes. GFP(+) CD133(+) cells had upregulated Wnt, FGF and BMP pathways and expressed neural crest markers. In GFP(-) CD133(+) cells, the hedgehog, IGF, Notch and integrin pathways were prominent. In skin reconstitution assays, hair follicles failed to form when dermis was depleted of both GFP(+) CD133(+) and GFP(-) CD133(+) cells. In the absence of GFP(+) CD133(+) cells, awl/auchene hairs failed to form and only zigzag hairs were found. We have thus demonstrated a previously unrecognised heterogeneity in dermal papilla cells and shown that Sox2-positive cells specify particular hair follicle types.

AB - The dermal papilla comprises the specialised mesenchymal cells at the base of the hair follicle. Communication between dermal papilla cells and the overlying epithelium is essential for differentiation of the hair follicle lineages. We report that Sox2 is expressed in all dermal papillae at E16.5, but from E18.5 onwards expression is confined to a subset of dermal papillae. In postnatal skin, Sox2 is only expressed in the dermal papillae of guard/awl/auchene follicles, whereas CD133 is expressed both in guard/awl/auchene and in zigzag dermal papillae. Using transgenic mice that express GFP under the control of the Sox2 promoter, we isolated Sox2(+) (GFP(+)) CD133(+) cells and compared them with Sox2(-) (GFP(-)) CD133(+) dermal papilla cells. In addition to the 'core' dermal papilla gene signature, each subpopulation expressed distinct sets of genes. GFP(+) CD133(+) cells had upregulated Wnt, FGF and BMP pathways and expressed neural crest markers. In GFP(-) CD133(+) cells, the hedgehog, IGF, Notch and integrin pathways were prominent. In skin reconstitution assays, hair follicles failed to form when dermis was depleted of both GFP(+) CD133(+) and GFP(-) CD133(+) cells. In the absence of GFP(+) CD133(+) cells, awl/auchene hairs failed to form and only zigzag hairs were found. We have thus demonstrated a previously unrecognised heterogeneity in dermal papilla cells and shown that Sox2-positive cells specify particular hair follicle types.

KW - Animals

KW - Antigens, CD

KW - Dermis

KW - Embryo, Mammalian

KW - Epidermis

KW - Gene Expression Profiling

KW - Gene Regulatory Networks

KW - Glycoproteins

KW - Hair Follicle

KW - Mice

KW - Mice, Inbred BALB C

KW - Mice, Nude

KW - Mice, Transgenic

KW - Oligonucleotide Array Sequence Analysis

KW - Peptides

KW - Recombinant Fusion Proteins

KW - SOXB1 Transcription Factors

KW - Signal Transduction

U2 - 10.1242/dev.038620

DO - 10.1242/dev.038620

M3 - Journal article

C2 - 19605494

SN - 1477-9129

VL - 136

SP - 2815

EP - 2823

JO - Development (Cambridge, England)

JF - Development (Cambridge, England)

IS - 16

ER -