Simultaneous hyperpolarized 13C-Pyruvate MRI and 18F-FDG PET (HyperPET) in 10 dogs with cancer

Henrik Gutte Borgwardt; Adam Espe Hansen; Majbrit M. E. Larsen; Sofie Rahbek; Sarah T. Henriksen; Helle Hjorth Johannesen; Jan Henrik Ardenkjær-Larsen; Annemarie Thuri Kristensen; Liselotte Højgaard; Andreas Kjær

doi:10.2967/jnumed.115.156364

Simultaneous hyperpolarized ¹³C-Pyruvate MRI and ¹⁸F-FDG PET (HyperPET) in 10 dogs with cancer

Henrik Gutte Borgwardt, Adam Espe Hansen, Majbrit M. E. Larsen, Sofie Rahbek, Sarah T. Henriksen, Helle Hjorth Johannesen, Jan Henrik Ardenkjær-Larsen, Annemarie Thuri Kristensen, Liselotte Højgaard, Andreas Kjær

29 Citations (Scopus)

Abstract

With the introduction of combined PET/MR spectroscopic (MRS) imaging, it is now possible to directly and indirectly image the Warburg effect with hyperpolarized (13)C-pyruvate and (18)F-FDG PET imaging, respectively, via a technique we have named hyperPET. The main purpose of this present study was to establish a practical workflow for performing (18)F-FDG PET and hyperpolarized (13)C-pyruvate MRS imaging simultaneously for tumor tissue characterization and on a larger scale test its feasibility. In addition, we evaluated the correlation between (18)F-FDG uptake and (13)C-lactate production.

METHODS: Ten dogs with biopsy-verified spontaneous malignant tumors were included for imaging. All dogs underwent a protocol of simultaneous (18)F-FDG PET, anatomic MR, and hyperpolarized dynamic nuclear polarization with (13)C-pyruvate imaging. The data were acquired using a combined clinical PET/MR imaging scanner.

RESULTS: We found that combined (18)F-FDG PET and (13)C-pyruvate MRS imaging was possible in a single session of approximately 2 h. A continuous workflow was obtained with the injection of (18)F-FDG when the dogs was placed in the PET/MR scanner. (13)C-MRS dynamic acquisition demonstrated in an axial slab increased (13)C-lactate production in 9 of 10 dogs. For the 9 dogs, the (13)C-lactate was detected after a mean of 25 s (range, 17-33 s), with a mean to peak of (13)C-lactate at 49 s (range, 40-62 s). (13)C-pyruvate could be detected on average after 13 s (range, 5-26 s) and peaked on average after 25 s (range, 13-42 s). We noticed concordance of (18)F-FDG uptake and production of (13)C-lactate in most, but not all, axial slices.

CONCLUSION: In this study, we have shown in a series of dogs with cancer that hyperPET can easily be performed within 2 h. We showed mostly correspondence between (13)C-lactate production and (18)F-FDG uptake and expect the combined modalities to reveal additional metabolic information to improve prognostic value and improve response monitoring.

Original language	English
Journal	Journal of Nuclear Medicine
Volume	56
Issue number	11
Pages (from-to)	1786-92
Number of pages	7
ISSN	0161-5505
DOIs	https://doi.org/10.2967/jnumed.115.156364
Publication status	Published - 1 Nov 2015

Keywords

cancer
dynamic nuclear polarization
hyperpolarized
13C-pyruvate
MR
18F-FDG PET
PET/MR
molecular imaging

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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Cite this

@article{13602fcb242941aba54f840d1dde46b0,

title = "Simultaneous hyperpolarized 13C-Pyruvate MRI and 18F-FDG PET (HyperPET) in 10 dogs with cancer",

abstract = "With the introduction of combined PET/MR spectroscopic (MRS) imaging, it is now possible to directly and indirectly image the Warburg effect with hyperpolarized (13)C-pyruvate and (18)F-FDG PET imaging, respectively, via a technique we have named hyperPET. The main purpose of this present study was to establish a practical workflow for performing (18)F-FDG PET and hyperpolarized (13)C-pyruvate MRS imaging simultaneously for tumor tissue characterization and on a larger scale test its feasibility. In addition, we evaluated the correlation between (18)F-FDG uptake and (13)C-lactate production.METHODS: Ten dogs with biopsy-verified spontaneous malignant tumors were included for imaging. All dogs underwent a protocol of simultaneous (18)F-FDG PET, anatomic MR, and hyperpolarized dynamic nuclear polarization with (13)C-pyruvate imaging. The data were acquired using a combined clinical PET/MR imaging scanner.RESULTS: We found that combined (18)F-FDG PET and (13)C-pyruvate MRS imaging was possible in a single session of approximately 2 h. A continuous workflow was obtained with the injection of (18)F-FDG when the dogs was placed in the PET/MR scanner. (13)C-MRS dynamic acquisition demonstrated in an axial slab increased (13)C-lactate production in 9 of 10 dogs. For the 9 dogs, the (13)C-lactate was detected after a mean of 25 s (range, 17-33 s), with a mean to peak of (13)C-lactate at 49 s (range, 40-62 s). (13)C-pyruvate could be detected on average after 13 s (range, 5-26 s) and peaked on average after 25 s (range, 13-42 s). We noticed concordance of (18)F-FDG uptake and production of (13)C-lactate in most, but not all, axial slices.CONCLUSION: In this study, we have shown in a series of dogs with cancer that hyperPET can easily be performed within 2 h. We showed mostly correspondence between (13)C-lactate production and (18)F-FDG uptake and expect the combined modalities to reveal additional metabolic information to improve prognostic value and improve response monitoring.",

keywords = "cancer, dynamic nuclear polarization, hyperpolarized, 13C-pyruvate, MR, 18F-FDG PET, PET/MR, molecular imaging",

author = "Borgwardt, {Henrik Gutte} and Hansen, {Adam Espe} and Larsen, {Majbrit M. E.} and Sofie Rahbek and Henriksen, {Sarah T.} and Johannesen, {Helle Hjorth} and Ardenkj{\ae}r-Larsen, {Jan Henrik} and Kristensen, {Annemarie Thuri} and Liselotte H{\o}jgaard and Andreas Kj{\ae}r",

note = "{\textcopyright} 2015 by the Society of Nuclear Medicine and Molecular Imaging, Inc.",

year = "2015",

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doi = "10.2967/jnumed.115.156364",

language = "English",

volume = "56",

pages = "1786--92",

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TY - JOUR

T1 - Simultaneous hyperpolarized 13C-Pyruvate MRI and 18F-FDG PET (HyperPET) in 10 dogs with cancer

AU - Borgwardt, Henrik Gutte

AU - Hansen, Adam Espe

AU - Larsen, Majbrit M. E.

AU - Rahbek, Sofie

AU - Henriksen, Sarah T.

AU - Johannesen, Helle Hjorth

AU - Ardenkjær-Larsen, Jan Henrik

AU - Kristensen, Annemarie Thuri

AU - Højgaard, Liselotte

AU - Kjær, Andreas

PY - 2015/11/1

Y1 - 2015/11/1

N2 - With the introduction of combined PET/MR spectroscopic (MRS) imaging, it is now possible to directly and indirectly image the Warburg effect with hyperpolarized (13)C-pyruvate and (18)F-FDG PET imaging, respectively, via a technique we have named hyperPET. The main purpose of this present study was to establish a practical workflow for performing (18)F-FDG PET and hyperpolarized (13)C-pyruvate MRS imaging simultaneously for tumor tissue characterization and on a larger scale test its feasibility. In addition, we evaluated the correlation between (18)F-FDG uptake and (13)C-lactate production.METHODS: Ten dogs with biopsy-verified spontaneous malignant tumors were included for imaging. All dogs underwent a protocol of simultaneous (18)F-FDG PET, anatomic MR, and hyperpolarized dynamic nuclear polarization with (13)C-pyruvate imaging. The data were acquired using a combined clinical PET/MR imaging scanner.RESULTS: We found that combined (18)F-FDG PET and (13)C-pyruvate MRS imaging was possible in a single session of approximately 2 h. A continuous workflow was obtained with the injection of (18)F-FDG when the dogs was placed in the PET/MR scanner. (13)C-MRS dynamic acquisition demonstrated in an axial slab increased (13)C-lactate production in 9 of 10 dogs. For the 9 dogs, the (13)C-lactate was detected after a mean of 25 s (range, 17-33 s), with a mean to peak of (13)C-lactate at 49 s (range, 40-62 s). (13)C-pyruvate could be detected on average after 13 s (range, 5-26 s) and peaked on average after 25 s (range, 13-42 s). We noticed concordance of (18)F-FDG uptake and production of (13)C-lactate in most, but not all, axial slices.CONCLUSION: In this study, we have shown in a series of dogs with cancer that hyperPET can easily be performed within 2 h. We showed mostly correspondence between (13)C-lactate production and (18)F-FDG uptake and expect the combined modalities to reveal additional metabolic information to improve prognostic value and improve response monitoring.

AB - With the introduction of combined PET/MR spectroscopic (MRS) imaging, it is now possible to directly and indirectly image the Warburg effect with hyperpolarized (13)C-pyruvate and (18)F-FDG PET imaging, respectively, via a technique we have named hyperPET. The main purpose of this present study was to establish a practical workflow for performing (18)F-FDG PET and hyperpolarized (13)C-pyruvate MRS imaging simultaneously for tumor tissue characterization and on a larger scale test its feasibility. In addition, we evaluated the correlation between (18)F-FDG uptake and (13)C-lactate production.METHODS: Ten dogs with biopsy-verified spontaneous malignant tumors were included for imaging. All dogs underwent a protocol of simultaneous (18)F-FDG PET, anatomic MR, and hyperpolarized dynamic nuclear polarization with (13)C-pyruvate imaging. The data were acquired using a combined clinical PET/MR imaging scanner.RESULTS: We found that combined (18)F-FDG PET and (13)C-pyruvate MRS imaging was possible in a single session of approximately 2 h. A continuous workflow was obtained with the injection of (18)F-FDG when the dogs was placed in the PET/MR scanner. (13)C-MRS dynamic acquisition demonstrated in an axial slab increased (13)C-lactate production in 9 of 10 dogs. For the 9 dogs, the (13)C-lactate was detected after a mean of 25 s (range, 17-33 s), with a mean to peak of (13)C-lactate at 49 s (range, 40-62 s). (13)C-pyruvate could be detected on average after 13 s (range, 5-26 s) and peaked on average after 25 s (range, 13-42 s). We noticed concordance of (18)F-FDG uptake and production of (13)C-lactate in most, but not all, axial slices.CONCLUSION: In this study, we have shown in a series of dogs with cancer that hyperPET can easily be performed within 2 h. We showed mostly correspondence between (13)C-lactate production and (18)F-FDG uptake and expect the combined modalities to reveal additional metabolic information to improve prognostic value and improve response monitoring.

KW - cancer

KW - dynamic nuclear polarization

KW - hyperpolarized

KW - 13C-pyruvate

KW - MR

KW - 18F-FDG PET

KW - PET/MR

KW - molecular imaging

U2 - 10.2967/jnumed.115.156364

DO - 10.2967/jnumed.115.156364

M3 - Journal article

C2 - 26338899

SN - 0161-5505

VL - 56

SP - 1786

EP - 1792

JO - Journal of Nuclear Medicine

JF - Journal of Nuclear Medicine

IS - 11

ER -