Significant improvement of optical traps by tuning standard water immersion objectives

Seyyed Nader Seyyed Reihani; Andrew Callum Richardson; Lene Broeng Oddershede

doi:10.1088/2040-8978/13/10/105301

Significant improvement of optical traps by tuning standard water immersion objectives

Seyyed Nader Seyyed Reihani, Andrew Callum Richardson, Lene Broeng Oddershede

17 Citations (Scopus)

Abstract

Focused infrared lasers are widely used for micromanipulation and visualization of biological specimens. An inherent practical problem is that off-the-shelf commercial microscope objectives are designed for use with visible and not infrared wavelengths. Less aberration is introduced by water immersion objectives than by oil immersion ones, however, even water immersion objectives induce significant aberration. We present a simple method to reduce the spherical aberration induced by water immersion objectives, namely by tuning the correction collar of the objective to a value that is ∼ 10% lower than the physical thickness of the coverslip. This results in marked improvements in optical trapping strengths of up to 100% laterally and 600% axially from a standard microscope objective designed for use in the visible range. The results are generally valid for any water immersion objective with any numerical aperture.

Original language	English
Journal	Journal of Optics
Volume	13
Issue number	10
Pages (from-to)	105301
ISSN	0972-8821
DOIs	https://doi.org/10.1088/2040-8978/13/10/105301
Publication status	Published - 1 Oct 2011

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title = "Significant improvement of optical traps by tuning standard water immersion objectives",

abstract = "Focused infrared lasers are widely used for micromanipulation and visualization of biological specimens. An inherent practical problem is that off-the-shelf commercial microscope objectives are designed for use with visible and not infrared wavelengths. Less aberration is introduced by water immersion objectives than by oil immersion ones, however, even water immersion objectives induce significant aberration. We present a simple method to reduce the spherical aberration induced by water immersion objectives, namely by tuning the correction collar of the objective to a value that is ∼ 10% lower than the physical thickness of the coverslip. This results in marked improvements in optical trapping strengths of up to 100% laterally and 600% axially from a standard microscope objective designed for use in the visible range. The results are generally valid for any water immersion objective with any numerical aperture.",

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AU - Seyyed Reihani, Seyyed Nader

AU - Richardson, Andrew Callum

AU - Oddershede, Lene Broeng

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Y1 - 2011/10/1

N2 - Focused infrared lasers are widely used for micromanipulation and visualization of biological specimens. An inherent practical problem is that off-the-shelf commercial microscope objectives are designed for use with visible and not infrared wavelengths. Less aberration is introduced by water immersion objectives than by oil immersion ones, however, even water immersion objectives induce significant aberration. We present a simple method to reduce the spherical aberration induced by water immersion objectives, namely by tuning the correction collar of the objective to a value that is ∼ 10% lower than the physical thickness of the coverslip. This results in marked improvements in optical trapping strengths of up to 100% laterally and 600% axially from a standard microscope objective designed for use in the visible range. The results are generally valid for any water immersion objective with any numerical aperture.

AB - Focused infrared lasers are widely used for micromanipulation and visualization of biological specimens. An inherent practical problem is that off-the-shelf commercial microscope objectives are designed for use with visible and not infrared wavelengths. Less aberration is introduced by water immersion objectives than by oil immersion ones, however, even water immersion objectives induce significant aberration. We present a simple method to reduce the spherical aberration induced by water immersion objectives, namely by tuning the correction collar of the objective to a value that is ∼ 10% lower than the physical thickness of the coverslip. This results in marked improvements in optical trapping strengths of up to 100% laterally and 600% axially from a standard microscope objective designed for use in the visible range. The results are generally valid for any water immersion objective with any numerical aperture.

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DO - 10.1088/2040-8978/13/10/105301

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VL - 13

SP - 105301

JO - Journal of Optics (India)

JF - Journal of Optics (India)

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Significant improvement of optical traps by tuning standard water immersion objectives

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